설명
Features and Benefits
Disposable Membrane Chromatography units are designed for the rapid selection of appropriate chemistry and method development. The Mustang membrane chromatography starter kit includes both strong anion and cation exchange membrane chromatography Acrodisc® units, as well as specific endotoxin removal Acrodisc units. These allow both the rapid selection of the appropriate chemistry for your application, and the opportunity to quickly screen for optimal process parameters to enhance and simplify your process method development.
Starter Kit Contents
- Mustang Q Membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles. Typical binding capacities for Acrodisc units with Mustang Q membranes are:
- DNA: 3.6 – 4.5 mg/Acrodisc or 20 – 25 mg/mL membrane volume
- BSA: 9 – 11 mg/Acrodisc or 50 – 60 mg/mL membrane volume
- Mustang S Membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles. Typical binding capacities for Acrodisc units with Mustang S membranes are:
- Lysozyme 8 – 9 mg/Acrodisc or 45 – 50 mg/mL membrane volume
- Mustang E Membrane membrane features propriety membrane surface modifications to effectively remove endotoxin from water, saline and buffer solutions.Typical binding capacities for Mustang E membranes are:
- Approximately 500,000 EU/Acrodisc unit or approximately 4 x 106 EU/mL membrane volume
- Under the following conditions:
- 0.9% sodium chloride
- Minimum challenge level: 10,000 EU/mL
- Minimum challenge volume: 60 mL
- Endotoxin source: Escherichia coli train 055:B5 (no dispersing agents).
응용분야
- The rapid identification of the correct membrane chemistry for a specific application
- Rapid screening of key process parameters
규격사양
Materials of Construction
| Media | Mustang membrane of appropriate chemistry |
| Housing | Polypropylene |
Membrane Bed Volume
| Mustang S and Q Membrane | 0.18 mL |
| Mustang E Membrane | 0.12 mL |
Connections
| Inlet | Female Luer-Lok* |
| Outlet | Male Slip Luer |
Operating Pressures
| Typical Differential Pressure | < 0.07 bard @ 1 mL/min (< 1.0 psid @ 1 mL/min) |
| Maximum Operating Pressure and Temperature | 5.5 bar (80 psi) at 21 – 24 ºC 2.1 bar (30 psi) at 60 °C |
성능
Mustang S Membrane
The conditions used to generate data for the graph above include buffer:
10 mM MES pH 5.5; salt: 1M NaCl in 10 mM MES pH 5.5; gradient: 0 to 1M NaCl in 50 column volume (cv); flow rate: 2.3 mL/min (13 cv/min).
A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
(flow rate [2.3 mL/min] X initial protein Lysozyme concentration [0.512 mg/mL] X time [8.0 min]) / membrane bed volume of Mustang S in 25 mm (1 in.) Acrodisc unit (0.18 mL)
Mustang Q Membrane
The conditions used to generate data for the resolution graph above include buffer:
25 mM Tris pH 8.0; salt: 1M NaCl in 25 mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column volume (cv); flow rate: 2.3 mL/min (13 cv/min); sample loading: 4% of total binding capacity.
A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occured at 8.1 minutes and was calculated as 54 mg/mL using:
(flow rate [2.3 mL/min] X initial protein BSA concentration [0.524 mg/mL] X time [8.1 min]) / membrane bed volume of Mustang Q in 25 mm (1 in.) Acrodisc unit (0.18 mL)
Mustang E Membrane
종류
사용
관련 문서
Regulatory/Quality Documentation
주문정보
Ordering Information
| Part Number | Description | Packaging |
| MSTG25KIT | Mustang Starter Kit |
4 per package Acrodisc 25 mm (1 in.) with 4 per package Acrodisc 25 mm (1 in.) with 2 per package Acrodisc 25 mm (1 in.) with |
Complementary Products
| Part Number | Description | Packaging |
| MSTG25Q6 | Acrodisc 25 mm (1 in.) with Mustang Q membrane | 10 per package |
| MSTG25S6 | Acrodisc 25 mm (1 in.) with Mustang S membrane | 10 per package |
| MSTG25E3 | Acrodisc 25 mm (1 in.) with Mustang E membrane | 10 per package |
| CLM05MSTGQP1 | Mustang Q Capsule, 10 mL bed volume | 1 per package |
| CLM05MSTGSP1 | Mustang S Capsule, 10 mL bed volume | 1 per package |
| CLM05MSTGEP1 | Mustang E Capsule, 10 mL bed volume | 1 per package |
| CL3MSTGQP1 |
Mustang Q Capsule, 60 mL bed volume |
1 per package |
| CL3MSTGSP1 | Mustang S Capsule, 60 mL bed volume | 1 per package |
| CL3MSTGEP1 | Mustang E Capsule, 40 mL bed volume | 1 per package |
| NP6MSTGQP1 | Mustang Q Capsule, 260 mL bed volume | 1 per package |
| NP6MSTGSP1 | Mustang S Capsule, 260 mL bed volume | 1 per package |
| NP6MSTGEP1 | Mustang E Capsule, 160 mL bed volume | 1 per package |
| NP7MSTGQP1 | Mustang Q Capsule, 520 mL bed volume | 1 per package |
| NP7MSTGSP1 | Mustang S Capsule, 520 mL bed volume | 1 per package |
| NP8MSTGQP1 | Mustang Q Capsule, 780 mL bed volume | 1 per package |
| NP8MSTGSP1 | Mustang S Capsule, 780 mL bed volume | 1 per package |
적용분야
영역
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