Biodyne® Nylon Transfer Membranes product photo Primary L

Biodyne® Nylon Transfer Membranes

High Sensitivity and Low Background
for Enhanced Detection and Resolution

  • Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
  • Membranes are intrinsically hydrophilic for easy wetting
  • Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."


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설명

High Sensitivity and Low Background
for Enhanced Detection and Resolution

  • Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
  • Membranes are intrinsically hydrophilic for easy wetting
  • Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."

응용분야

Four Chemistries Provide Versatile Adsorption Properties

Biodyne A Membrane

  • (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

Biodyne B Membrane

  • (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.

Biodyne C Membrane

  • (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

Biodyne Plus Membrane

  • (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

규격사양

Filter Media

  • Biodyne A Membrane: Amphoteric Nylon 6,6
  • Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
  • Biodyne C Membrane: Negativelycharged Nylon 6,6

Pore Size

  • 0.2, 0.45, and 1.2 μm

Typical Thickness

 
Membrane μm mils
Biodyne A 5.5 - 7.0 139.7 - 177.8
Biodyne B 5.7 - 6.7 144.8 - 170.2
Biodyne Plus 5.7 - 6.7 144.8 - 170.2
Biodyne C 11.0 - 13.01 279.4 - 330.2
 

Solvent Compatibility

  • Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatible with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

1Dual layer measurements

성능

Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing


Biodyne B Membrane Withstands Stripping and Reprobing
Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 

1A - 4A: blot after (re)probing
1B - 4B: blot after stripping, prior to re(probing)

Fluorescent Detection of DNA Using Biodyne Plus Membrane


Fluorescent Detection of DNA Using Biodyne Plus Membrane
ng total DNA

Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.

종류

Membrane Filters

사용

Binding

적용분야

Northern & Southern Blotting

영역

DNA & RNA Purification

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