Resolute® FM Chromatography Columns product photo Primary L

Resolute® FM Chromatography Columns

100-200 mm I.D. Chromatography Columns for Pilot or Production Use

Resolute FM columns are ideal for chromatographic purification from process development through to manufacturing scale with a wide range of commonly used media. These glass and acrylic columns, for development and manufacturing applications, are robust and incorporate new features to improve the outcome of column packing events. The industry accepted constant compression seal design used in the successful Resolute DM range is also incorporated in the FM series. The columns are ideal for use with Pall’s PKP process controllers providing an ideally suited platform for pilot scale and small volume manufacturing purification operations with a wide range of chromatographic media. Accessory column valves and tubing kits are available to provide a complete solution for your next purification challenge.  

View Resolute large scale process columns.

Key Features and Benefits

  • Sanitary design
  • Tilting mechanism for air removal
  • Ability to raise adjuster eliminates manual handling issues
  • Flexible unpacking from top or bottom of the column
  • Acrylic option available for glass sensitive proteins

Modular Design

Resolute FM Column Module DesignResolute FM columns can be packed and unpacked with the column adjuster remaining supported above the top of the tube. The central adjuster mechanism enables the top distribution cell to travel within the tube and to be raised clear of the tube for packing and unpacking operations. Thus eliminating the requirement to remove and lay down the adjuster and associated tubing. This improves operator safety and reduces preparation time with connected hoses being left in place.

The adjuster seal positioned close to the edge of the distribution plate is under constant compression within the tube. There is no need to adjust and fine tune seal compression sealing is assured every time without operator involvement. This feature avoids problems associated with seals being either under or over compressed. This avoids leakage of fluids past the seal during the lowering of the adjuster during axial bed compression.

Unlike other column designs, the adjuster is always maintained in a relative vertical plane to the column tube by the adjuster plate and does not need to be inserted by the operator at an angle into the tube to remove air while the seal is formed against the tube wall. Resolute FM columns have a unique feature on the lower frame which allows the column assembly to be tilted from vertical while the adjuster is lowered. This feature has simplified the packing preparation process and results in a reproducible outcome which can be defined in SOP’s and avoids awkward handling of heavy column components and attached tubing. This procedure is ideal for concise SOP’s and produces reproducible column priming outcomes due the security of the mechanical steps involved in place of manual manipulation of separate column parts.

Fast Packing and Unpacking

Resolute FM Chromatography ColumnThe design of the central adjuster mechanism provides clearance for entry of the slurry directly into the column tube. With a measured volume of slurry in the column, the liquid level is then topped up with buffer to a specific bed height (marked on the tube unit). The column is then set in the priming position and the adjuster lowered into the tube by rotating the centrally located handles. As the seals enter the tube the air is displaced, and once the seal is made the column is again set vertical before applying the required flow to pack the bed. Once the bed is compressed to a target value the flow is stopped and the adjuster lowered to achieve an optimal pack.

Unpacking can be performed through the bottom or top of the tube. Typically, the adjuster is raised by rotating the centrally located handles until it is clear of the tube. After rinsing the top distribution plate and net to recover residual media, the column contents are gently agitated with buffer to form a slurry before being pumped to a container.

 
 
 


Click here for Resolute automated packing solutions



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Columns (Adjustable Capacity)

Acrylic, 100 mm diameter
List Price
$ 10,316.00
Acrylic, 100 mm diameter
List Price
$ 11,114.00
Glass, 100 mm diameter
List Price
$ 8,727.00
Glass, 200 mm diameter
List Price
$ 11,923.00
Description

100-200 mm I.D. Chromatography Columns for Pilot or Production Use

Resolute FM columns are ideal for chromatographic purification from process development through to manufacturing scale with a wide range of commonly used media. These glass and acrylic columns, for development and manufacturing applications, are robust and incorporate new features to improve the outcome of column packing events. The industry accepted constant compression seal design used in the successful Resolute DM range is also incorporated in the FM series. The columns are ideal for use with Pall’s PKP process controllers providing an ideally suited platform for pilot scale and small volume manufacturing purification operations with a wide range of chromatographic media. Accessory column valves and tubing kits are available to provide a complete solution for your next purification challenge.  

View Resolute large scale process columns.

Key Features and Benefits

  • Sanitary design
  • Tilting mechanism for air removal
  • Ability to raise adjuster eliminates manual handling issues
  • Flexible unpacking from top or bottom of the column
  • Acrylic option available for glass sensitive proteins

Modular Design

Resolute FM Column Module DesignResolute FM columns can be packed and unpacked with the column adjuster remaining supported above the top of the tube. The central adjuster mechanism enables the top distribution cell to travel within the tube and to be raised clear of the tube for packing and unpacking operations. Thus eliminating the requirement to remove and lay down the adjuster and associated tubing. This improves operator safety and reduces preparation time with connected hoses being left in place.

The adjuster seal positioned close to the edge of the distribution plate is under constant compression within the tube. There is no need to adjust and fine tune seal compression sealing is assured every time without operator involvement. This feature avoids problems associated with seals being either under or over compressed. This avoids leakage of fluids past the seal during the lowering of the adjuster during axial bed compression.

Unlike other column designs, the adjuster is always maintained in a relative vertical plane to the column tube by the adjuster plate and does not need to be inserted by the operator at an angle into the tube to remove air while the seal is formed against the tube wall. Resolute FM columns have a unique feature on the lower frame which allows the column assembly to be tilted from vertical while the adjuster is lowered. This feature has simplified the packing preparation process and results in a reproducible outcome which can be defined in SOP’s and avoids awkward handling of heavy column components and attached tubing. This procedure is ideal for concise SOP’s and produces reproducible column priming outcomes due the security of the mechanical steps involved in place of manual manipulation of separate column parts.

Fast Packing and Unpacking

Resolute FM Chromatography ColumnThe design of the central adjuster mechanism provides clearance for entry of the slurry directly into the column tube. With a measured volume of slurry in the column, the liquid level is then topped up with buffer to a specific bed height (marked on the tube unit). The column is then set in the priming position and the adjuster lowered into the tube by rotating the centrally located handles. As the seals enter the tube the air is displaced, and once the seal is made the column is again set vertical before applying the required flow to pack the bed. Once the bed is compressed to a target value the flow is stopped and the adjuster lowered to achieve an optimal pack.

Unpacking can be performed through the bottom or top of the tube. Typically, the adjuster is raised by rotating the centrally located handles until it is clear of the tube. After rinsing the top distribution plate and net to recover residual media, the column contents are gently agitated with buffer to form a slurry before being pumped to a container.

 
 
 


Click here for Resolute automated packing solutions

Applications
The superior design of Pall’s Resolute columns is optimized for purification in both process optimization and small scale manufacturing operations. These columns have proven performance with a wide range of chromatography media and chromatographic modes including: ion exchange, mixed mode, affinity, size exclusion and hydrophobic interaction. Column bed heights up to 27 cm may be packed with a compression factor of 1.2.
Specifications

General Specifications

 
Product Diameter (mm) Adjustable Height Range (mm) Adjustable Capacity Packed Volume (L)*  Maximum Allowable Pressure (barg/psig) 
Resolute FM 100 100   100 - 460  0.78 - 2.10 5.0/72.5 
Resolute FM 140  140  100 - 460  1.54 - 4.10 5.0/72.5 
Resolute FM 200 200   100 - 460  3.14 - 8.40 4.6/66.7 
 
 
Product Net Weight (kg/lb) Column Height at Minimum Bed (mm) Width (mm)  Depth (mm) Height (mm)
Resolute FM 100 30/66  1105  385  385  1625 
Resolute FM 140 45/99  1135  415  415  1655 
Resolute FM 200 70/154  1135  455  455  1655 
 
*Based on 70% slurry and compression factor x 1.2

Temperature

  • 2 - 35 ºC

Column Termination Sizes

 
  T1 mm T2 mm
Diameter (mm) 100  25 
Diameter (mm) 140  25 
Diameter (mm) 200  25 
 
 

Component Weights Kg (lb)

 
  FM100 FM140 FM200
Column  30.0 (66.1)  46.0 (101.4)  62.0 (136.7) 
Adjuster Assembly Complete  8.6 (19.0)  18.6 (41.0)  25.1 (55.3) 
 

Materials of Construction - Process Wetted Components

  • Tube:
    • Glass, borosilicate 3.3
    • Acrylic
  • Pipework / Distribution Plate
    • Stainless Steel, grade 316 L (EN 1.4404)
  • Nets
    • 20 μm – Polypropylene 10 μm – Polyamide
  • Net Support
    • Polypropylene
  • Seals
    • EPDM, black, peroxide cured

Materials of Construction - External Components

  • Tube
    • Glass, borosilicate 3.3
    • Acrylic
  • Seals:
    • EPDM, black, peroxide cured
  • Pressure Envelope Components
    • Stainless steel, grade 316 L (EN 1.4404)
    • PEEK
  • Top Flange
    • Polypropylene
  • Stand
    • Stainless steel, grade 316 L (EN 1.4404)
  • Castors
    • Stainless steel, grade 304
  • Wheels
    • Acetal

Materials used in the manufacture of the column product contacting components either conform to relevant sections of the FDA Code of Federal Regulations Volume 21, parts 170 to 199, or are non-FDA recognized materials that pass USP Class VI tests for toxicity. All Wetted Elastomers Conform to USP VI or CFR 177.2600.

Applicable Codes and Standards

 
Design Standard:  
Steel Components PD5500 (2012)
Acrylic tube PED Material Appraisal
Mechanical Design  PED 97/23/EC 
 P.E.D. Category SEP
 

Chemical Compatibility

The wetted materials of construction of Resolute FM are industry standard and are compatible with the majority of aqueous solutions used in the biotechnology industry. If you have queries about a particular application, please contact your local sales representative.
Performance

Figure 1. Packed Bed Performance

Acetone 2% (w/v), injection volume 1-5% CV, bed height 20 cm.


FM100

FM140

 

FM200

Sanitisation

Resolute FM columns are suitable for sanitization in empty or packed mode, as well as having easy access to all seals and supports for fast maintenance requirements. With a minimal number of parts, access for wipe down and the electropolished stainless steel surface finish minimizes entrapment of contaminants and reduces the risk of corrosion. Validation of all column sizes demonstrated that the columns can be effectively sanitized.

Ordering Information

Adjustable Capacity Columns

 
Item Description Diameter
(mm)
Resolute FM, Glass Resolute FM, Acrylic
FM 100/100-460 100 FM100A100A20D FM100AC100A20D
FM 140/100-460 140 FM140A100A20D FM140AC100A20D
FM 200/100-460 200 FM200A100A20D FM200AC100A20D
 
NOTES: Column ships with 20 μm nets fitted.

Spares Kits and Accessories

 
Column
Ø (mm)
Tube Kit, Glass Tube Kit, Acrylic Fixed & Adjuster
Net (10 μm)
Fixed & Adjuster
Net (20 μm)
Adjuster Net
Support
Fixed Net
Support1
Wetted
Seals2
Non Wetted
Seals3
100 SFM100-A520A99 SFM100-AC520A99 SFM100-A150A99 SFM100-A160A99 SFM100-A350A99 SFM100-A360A99 SFM100-A300A99 SFM100-A340A99
140 SFM140-A520A99 SFM140-AC520A99 SFM140-A150A99 SFM140-A160A99 SFM140-A350A99 SFM140-A360A99 SFM140-A300A99 SFM140-A340A99
200 SFM200-A520A99 SFM200-AC520A99 SFM200-A150A99 SFM200-A160A99 SFM200-A350A99 SFM200-A360A99 SFM200-A300A99 SFM200-A340A99
 

Accessories

 
  Accessories Tools
Column
Ø (mm)
Column
Valve Kit4
Bottom Valve
Mounting Kit5
Foot Kit Tubing Kit6 Deep Socket7 Stirring Paddle
100 SFM100-A270A99 SFM100-A320A99 SFM100-A310A99 SFM100-A420A99 SFM100-A330A99 SFM100-A390A99
140 SFM140-A270A99 SFM140-A320A99 SFM100-A310A99 SFM140-A420A99 SFM140-A330A99 SFM100-A390A99
200 SFM140-A270A99 SFM140-A320A99 SFM100-A310A99 SFM140-A420A99 SFM140-A330A99 SFM100-A390A99
 
1. Net support (1) to fit between net and distributor
2. Adjuster seal (1), tube seal (2) , inlet tube seal (1)
3. Adjuster nut flange seal (1), inlet spool top seals (2)
4. Two way valve (1), clamp (1), gasket (1) [2 valve kits required per column for top and bottom]
5. Bottom elbow (1), clamp and gasket (1), bracket (1) and bolts/washers (2)
6. Reinforced tubing (2 x 150 cm, 1 x 200 cm) with hose barbs (6), gaskets (6) and clamps (6)
7. Required when dismantling tube assembly

Installation and Validation Documentation

  • Each column is supplied with a user manual
  • A comprehensive validation and support package is also supplied, including:
    • Setup and test report
    • Materials and compatibility data
    • Comprehensive parts list
Application
Columns - Standard
Segment
Chromatography