AcroPrep™ Advance 96-Well Filter Plates With Mustang Q/S Ion Exchange Membrane product photo Primary L

AcroPrep™ Advance 96-Well Filter Plates With Mustang Q/S Ion Exchange Membrane

  • Mustang® membrane is able to withstand high flow rates to render fast purification of biomolecules
  • Well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume
  • Outlet tip geometry minimizes sample leakage and loss during incubation steps
  • Intrinsic plate properties prevent target molecules from binding to the plate


* Required
Need Help?
Download Datasheet
Description
  • Mustang® membrane is able to withstand high flow rates to render fast purification of biomolecules
  • Well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume
  • Outlet tip geometry minimizes sample leakage and loss during incubation steps
  • Intrinsic plate properties prevent target molecules from binding to the plate
Applications
 
  • High throughput protein purification
  • Screening of chromatography membranes
  • Screening of chromatography conditions
  • Protein fractionation
  • Antibody purification
Specifications
 

Chemistry and Materials of Construction

  • Filter Media: Supor (polyethersulfone)
  • Chromatography membrane : Mustang Q (anion exchange), and Mustang S (cation exchange) membranes, equivalent to 14 µL/well
  • Plate Housing: Polypropylene
  • Lid: Polystyrene

 

Dimensions

  • Length: 12.8 cm (5.0 in.)
  • Width: 8.6 cm (3.4 in.)
  • Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
  • Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.)

 

Well-Bottom Area

  • 0.25 cm2

 

Recommended Working Volume

  • 350 μL: < 300 μL
  • 1 mL: < 900 μL

 

Recommended Operating Vacuum

  • > 25.4 cm Hg (10 in. Hg)

 

Recommended Centrifugal Force

  • 1,500 x g

 

Typical Processing Time

    • Vacuum: 14 seconds
    • Centrifuge: < 2 minutes

 

Typical Hold-Up Volume

    • Vacuum: 21 μL
    • Centrifuge: 12 μL
Performance
 

Mustang Q and S Membrane Provide a High Protein Binding Capacity




Binding capacity of Mustang Q membrane, 0.38 mg/well (n=5), was determined with BSA in 50 mM Tris, pH 8.5. Mustang S membrane binding capacity, 0.29 mg/well (n=6), was determined using Lysozyme in 10 mM MES, pH 5.5.
Ordering Information
Part Number Description Pkg
AcroPrep Advance Filter Plates for Protein Purification
8071 350 μL, Mustang Q membrane 10/pkg
8072 350 μL, Mustang S membrane 10/pkg
8171 1 mL, Mustang Q membrane 5/pkg
8172 1 mL, Mustang S membrane 5/pkg
Accessories and Replacement Parts
5017 Multi-well plate vacuum manifold 1/pkg
 
Application
Membranes