Mustang® Chromatography Products product photo Primary L

Mustang® Chromatography Products

Combining high-capacity, ion-exchange Mustang membranes with a unique 16-layer open-pleat design results in a high-flow rate, high-capacity, single-use, versatile range of pleated products that can be used for a wide range of applications.

This 16-layer construction is held constant from the laboratory-scale Mustang XT Acrodisc Unit to the largest industrial process-scale capsule, ensuring constant chromatography bed depth in all formats. Mustang E capsules use three layers of a standard pleat format membrane to remove endotoxin from process feedstreams. The three-layer construction is held constant from the Mustang E Acrodisc® format to the largest industrial process scale capsule.

The ease of linear scale-up ensures a shortened process development time by significantly reducing the re-optimization required between scale-up steps. These units are specifically designed for single-use. This eliminates cleaning and the costs associated with cleaning validation. Mustang capsules are fully self-contained units that connect directly into a system using 18 mm (1.5 in.) sanitary flange connectors. Mustang cartridges require the use of a Code 7 housing which requires cleaning and reassembly after each use.

 

Mustang Q Membrane

Mustang Q membrane is an anion-exchange support with pendant quaternary amine functional groups in a cross-linked polymeric coating on a 0.8 micron pore-size membrane. This gives high dynamic capacities for high molecular weight products such as DNA, plasmids or even particles as large as viruses.

Mustang S Membrane

Mustang S membrane is a cation-exchange support with pendant sulfonic functional groups in a cross-linked polymeric coating on a 0.65 µm pore-size membrane. This gives high dynamic capacities for high molecular weight products such as IgG, Factor VIII and some viruses.

 

Mustang E Membrane

Mustang E membrane is a highly cross-linked quaternized amine charge polymer coating on a 0.2 micron pore size membrane. This gives very high dynamic capacities, under selected conditions for the removal of endotoxin from process feedstreams, buffers and water.

 

Features and Benefits

  • Binding Efficiency: charged biomolecules are readily bound in a single pass
  • Speed: high-flow rates enable the processing of large volumes in less than a single working shift. Mustang units typically operate at flow rates between 10 to 40 membrane volumes per minute, making them much faster than conventional columns which typically operate at 0.5 column volumes per minute
  • Scalability: full range of sizes accommodates the different volumes and capacities required in biopharmaceutical processing
  • Convenience: ready to use, autoclavable and disposable to eliminate potential packing, cleaning, validation and cross-contamination issues
  • Flexible: available in capsule or cartridge format
  • Cost: lower operating costs and capital investment compared to conventional columns that need validated packing and cleaning

High Quality Standards

  • Manufactured to high quality assurance standards in accordance with ISO 9000
  • Membrane lots tested for dynamic protein binding capacity, and peak position using standard proteins
  • Identified by lot number and unique serial number for complete traceability of manufacturing history, satisfying stringent QC/QA requirements
  • Supplied with Certificate of Analysis to confirm quality control
  • Meets USP Biological reactivity tests in vivo in accordance with USP Class VI 50 ºC and all materials listed in Drug Master File submitted to the FDA

Comprehensive Validation

  • Extensive validation to ensure consistent and reliable performance
  • A comprehensive validation guide is available for each of the membrane chemistries on request

Products in this datasheet may be covered by one or more patents including :
EP 1 189 685
US 6,849,185
US 7,189,322



* Required

Capsules

List Price
$ 1,210.00
List Price
$ 1,220.00
List Price
$ 1,220.00
List Price
$ 530.00
List Price
$ 530.00
List Price
$ 530.00
Description
Combining high-capacity, ion-exchange Mustang membranes with a unique 16-layer open-pleat design results in a high-flow rate, high-capacity, single-use, versatile range of pleated products that can be used for a wide range of applications.

This 16-layer construction is held constant from the laboratory-scale Mustang XT Acrodisc Unit to the largest industrial process-scale capsule, ensuring constant chromatography bed depth in all formats. Mustang E capsules use three layers of a standard pleat format membrane to remove endotoxin from process feedstreams. The three-layer construction is held constant from the Mustang E Acrodisc® format to the largest industrial process scale capsule.

The ease of linear scale-up ensures a shortened process development time by significantly reducing the re-optimization required between scale-up steps. These units are specifically designed for single-use. This eliminates cleaning and the costs associated with cleaning validation. Mustang capsules are fully self-contained units that connect directly into a system using 18 mm (1.5 in.) sanitary flange connectors. Mustang cartridges require the use of a Code 7 housing which requires cleaning and reassembly after each use.

 

Mustang Q Membrane

Mustang Q membrane is an anion-exchange support with pendant quaternary amine functional groups in a cross-linked polymeric coating on a 0.8 micron pore-size membrane. This gives high dynamic capacities for high molecular weight products such as DNA, plasmids or even particles as large as viruses.

Mustang S Membrane

Mustang S membrane is a cation-exchange support with pendant sulfonic functional groups in a cross-linked polymeric coating on a 0.65 µm pore-size membrane. This gives high dynamic capacities for high molecular weight products such as IgG, Factor VIII and some viruses.

 

Mustang E Membrane

Mustang E membrane is a highly cross-linked quaternized amine charge polymer coating on a 0.2 micron pore size membrane. This gives very high dynamic capacities, under selected conditions for the removal of endotoxin from process feedstreams, buffers and water.

 

Features and Benefits

  • Binding Efficiency: charged biomolecules are readily bound in a single pass
  • Speed: high-flow rates enable the processing of large volumes in less than a single working shift. Mustang units typically operate at flow rates between 10 to 40 membrane volumes per minute, making them much faster than conventional columns which typically operate at 0.5 column volumes per minute
  • Scalability: full range of sizes accommodates the different volumes and capacities required in biopharmaceutical processing
  • Convenience: ready to use, autoclavable and disposable to eliminate potential packing, cleaning, validation and cross-contamination issues
  • Flexible: available in capsule or cartridge format
  • Cost: lower operating costs and capital investment compared to conventional columns that need validated packing and cleaning

High Quality Standards

  • Manufactured to high quality assurance standards in accordance with ISO 9000
  • Membrane lots tested for dynamic protein binding capacity, and peak position using standard proteins
  • Identified by lot number and unique serial number for complete traceability of manufacturing history, satisfying stringent QC/QA requirements
  • Supplied with Certificate of Analysis to confirm quality control
  • Meets USP Biological reactivity tests in vivo in accordance with USP Class VI 50 ºC and all materials listed in Drug Master File submitted to the FDA

Comprehensive Validation

  • Extensive validation to ensure consistent and reliable performance
  • A comprehensive validation guide is available for each of the membrane chemistries on request

Products in this datasheet may be covered by one or more patents including :
EP 1 189 685
US 6,849,185
US 7,189,322

Specifications

Mustang Capsules 

Materials of Construction

 
Item CLxMSTG*P1 NPxMSTG*P1
Membrane Modified hydrophilic polyethersulfone Modified hydrophilic polyethersulfone
Membrane Support and Drainage Layer Assembly Polypropylene Polypropylene
Core and Cage Polypropylene Polypropylene
Endcaps and Adapters Polyester Polyester
Housing Bowl Polyetherimide Polypropylene
Housing Head Polyetherimide with TiO2 Polypropylene with TiO2
O-rings Silicone elastomer Silicone elastomer
 

Note:
For CLxMST*P1, x =
M05 for a Q, S or E unit with a bed volume of 10 mL
3 for a Q or S unit with a bed volume of 60 mL or an E unit with a bed volume of 40 mL

For NPxMSTG*P1, x =
6 for a Q or S unit with a bed volume of 260 mL or an E unit with a bed volume of 160 mL
7 for a Q or S unit with a bed volume of 520 mL or an E unit with a bed volume of 320 mL
8 for a Q or S unit with a bed volume of 780 mL or an E unit with a bed volume of 480 mL

* =
Q for a unit with Q (quaternary amine) chemistry membrane
S for a unit with S (sulfonic acid) chemistry membrane
E for a unit with E (polyethyleneimine) chemistry membrane

Nominal Dimensions

 
Capsule Type CLM05MSTG*P1 CL3MSTG*P1 NP6MPSTG*P1 NP7MSTG*P1 NP8MSTG*P1
Maximum Diameter (inc. Valves) 123 mm (4.8 in.) 123 mm (4.8 in.) 154 mm (6.1 in.) 154 mm (6.1 in.) 154 mm (6.1 in.)
Length with Sanitary Flange Fittings 84 mm (3.3 in.) 157 mm (6.2 in.) 335 mm (13.2 in.) 581 mm (22. 9 in.) 831 mm (32.7 in.)
Bed Volume 10 mL Q and S = 60 mL
E = 40 mL
Q and S = 260 mL
E = 160 mL
Q and S = 520 mL
E = 320 mL
Q and S = 780 mL
E = 480 mL
 

* Q for a unit with Q (quaternary amine) chemistry membrane; S for a unit with S (sulfonic acid) chemistry membrane; E for a unit with E (polyethyleneimine) chemistry membrane 

Operating Characteristics¹

 
Maximum Operating Pressure; Maximum Differential Pressure 4.1 bar (59.5 psi) at 38 °C
Maximum Sanitization Conditions 1 N NaOH for 30 minutes for one cycle only
Maximum Autoclave Conditions 121° C for 30 minutes for one cycle only
 

¹ With fully compatible fluids that do not soften, swell or adversely affect the capsule or its materials of construction

Typical Binding Characteristics

 
Chemistry Molecule MW or Size CLM05 CL3 NP6 NP7 NP8
Q BSA 65 kD 0.5 – 0.6 g 3.0 – 3.6 g 13 – 16 g 26 – 31 g 39 – 47 g
Q Thyroglobulin 650 kD 0.3 g 1.8 g 7.8 g 15.6 g 23.4 g
DNA - 0.20 – 0.25 g 1.2 – 1.5 g 5.2 – 6.5 g 10.4 – 13.0 g 15.6 – 19.5 g
Plasmid DNA 4.5 kb plasmid 0.15 g 0.9 g 3.9 g 7.8 g 11.7 g
Plasmid DNA 12 kb plasmid 0.15 g 0.9 g 3.9 g 7.8 g 11.7 g
Adenovirus 70 – 90 nm 5 x 1013 viral particles 3 x 1014 viral particles 1.3 x 1015 viral particles 2.6 x 1015 viral particles 3.9 x 1015 viral particles
Lysozyme 14.3 kD 0.45 – 0.50 g 2.7 – 3.0 g 11.7 – 13.0 g 23.4 – 26.0 g 35.1 – 39.0 g
S Human IgG 160 kD 0.2 g 1.2 g 5.2 g 10.4 g 15.6 g
E Endotoxin Units   4 x 107 EU/capsule 1.6 x 108 EU/capsule 6.4 x 108 EU/capsule 1.28 x 109 EU/capsule 1.92 x 109 EU/capsule
 
Ordering Information

Mustang Capsules

 
Chemistry Bed Volume Pack Quantity Part Number Description
Q 10 mL 1 CLM05MSTGQP1 38 mm (1.5 in.) sanitary connectors, in-line capsule
Q 60 mL CL3MSTGQP1 38 mm (1.5 in.) sanitary connectors, in-line capsule
Q 260 mL 1 NP6MSTGQP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
Q 520 mL 1 NP7MSTGQP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
Q 780 mL 1 NP8MSTGQP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
S 10 mL 1 CLM05MSTGSP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 

60 mL 1 CL3MSTGSP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
S 260 mL 1 NP6MSTGSP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
S 520 mL NP7MSTGSP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
S 780 mL 1 NP8MSTGSP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
10 mL 1 CLM05MSTGEP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
E 40 mL 1 CL3MSTGEP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
160 mL 1 NP6MSTGEP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
320 mL 1 NP7MSTGEP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
E 480 mL 1 NP8MSTGEP1 38 mm (1.5 in.) sanitary connectors, in-line capsule 
 
Application
Membranes
Segment
Chromatography