Mustang® Membrane Chromatography Starter Kits product photo Primary L

MustangĀ® Membrane Chromatography Starter Kits

Features and Benefits

Disposable Membrane Chromatography units are designed for the rapid selection of appropriate chemistry and method development. The Mustang membrane chromatography starter kit includes both strong anion and cation exchange membrane chromatography Acrodisc® units, as well as specific endotoxin removal Acrodisc units. These allow both the rapid selection of the appropriate chemistry for your application, and the opportunity to quickly screen for optimal process parameters to enhance and simplify your process method development.

Starter Kit Contents

  • Mustang Q Membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles. Typical binding capacities for Acrodisc units with Mustang Q membranes are:
    • DNA: 3.6 – 4.5 mg/Acrodisc or 20 – 25 mg/mL membrane volume
    • BSA: 9 – 11 mg/Acrodisc or 50 – 60 mg/mL membrane volume
  • Mustang S Membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles. Typical binding capacities for Acrodisc units with Mustang S membranes are:
    • Lysozyme 8 – 9 mg/Acrodisc or 45 – 50 mg/mL membrane volume
  • Mustang E Membrane membrane features propriety membrane surface modifications to effectively remove endotoxin from water, saline and buffer solutions.Typical binding capacities for Mustang E membranes are:
    • Approximately 500,000 EU/Acrodisc unit or approximately 4 x 106 EU/mL membrane volume
  • Under the following conditions: 
    • 0.9% sodium chloride
    • Minimum challenge level: 10,000 EU/mL
    • Minimum challenge volume: 60 mL
    • Endotoxin source: Escherichia coli train 055:B5 (no dispersing agents).


* Required

Complementary Products

Acrodisc unit with Mustang Q membrane, 0.8 µm, 25 mm, non-sterile (10/pkg (blister packs))
List Price
$ 216.62
Acrodisc unit with Mustang S membrane, 0.8 µm, 25 mm, non-sterile (10/pkg (blister packs))
List Price
$ 216.62

Complementary Products

0.2 μm, 25 mm, sterile (10/pkg (blister packs))
List Price
$ 200.92
Description

Features and Benefits

Disposable Membrane Chromatography units are designed for the rapid selection of appropriate chemistry and method development. The Mustang membrane chromatography starter kit includes both strong anion and cation exchange membrane chromatography Acrodisc® units, as well as specific endotoxin removal Acrodisc units. These allow both the rapid selection of the appropriate chemistry for your application, and the opportunity to quickly screen for optimal process parameters to enhance and simplify your process method development.

Starter Kit Contents

  • Mustang Q Membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles. Typical binding capacities for Acrodisc units with Mustang Q membranes are:
    • DNA: 3.6 – 4.5 mg/Acrodisc or 20 – 25 mg/mL membrane volume
    • BSA: 9 – 11 mg/Acrodisc or 50 – 60 mg/mL membrane volume
  • Mustang S Membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles. Typical binding capacities for Acrodisc units with Mustang S membranes are:
    • Lysozyme 8 – 9 mg/Acrodisc or 45 – 50 mg/mL membrane volume
  • Mustang E Membrane membrane features propriety membrane surface modifications to effectively remove endotoxin from water, saline and buffer solutions.Typical binding capacities for Mustang E membranes are:
    • Approximately 500,000 EU/Acrodisc unit or approximately 4 x 106 EU/mL membrane volume
  • Under the following conditions: 
    • 0.9% sodium chloride
    • Minimum challenge level: 10,000 EU/mL
    • Minimum challenge volume: 60 mL
    • Endotoxin source: Escherichia coli train 055:B5 (no dispersing agents).
Applications
  • The rapid identification of the correct membrane chemistry for a specific application
  • Rapid screening of key process parameters
Specifications

Materials of Construction

 
Media Mustang membrane of appropriate chemistry
Housing Polypropylene
 

Membrane Bed Volume

 
Mustang S and Q Membrane  0.18 mL
Mustang E Membrane  0.12 mL
 

Connections

 
Inlet Female Luer-Lok*
Outlet Male Slip Luer
 
* Luer-Lok is trademark of Becton-Dickenson & Co.

Operating Pressures

 
Typical Differential Pressure < 0.07 bard @ 1 mL/min
(< 1.0 psid @ 1 mL/min)
Maximum Operating Pressure and Temperature 5.5 bar (80 psi) at 21 – 24 ºC
2.1 bar (30 psi) at 60 °C
 
Performance
The following charts and graphs demonstrate the high resolution and high dynamic binding capacities of Mustang membrane chromatography units.

Mustang S Membrane

    

The conditions used to generate data for the graph above include buffer:
10 mM MES pH 5.5; salt: 1M NaCl in 10 mM MES pH 5.5; gradient: 0 to 1M NaCl in 50 column volume (cv); flow rate: 2.3 mL/min (13 cv/min).

 

A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
(flow rate [2.3 mL/min] X initial protein Lysozyme concentration [0.512 mg/mL] X time [8.0 min]) / membrane bed volume of Mustang S in 25 mm (1 in.) Acrodisc unit (0.18 mL)

Mustang Q Membrane

 

The conditions used to generate data for the resolution graph above include buffer:
25 mM Tris pH 8.0; salt: 1M NaCl in 25 mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column volume (cv); flow rate: 2.3 mL/min (13 cv/min); sample loading: 4% of total binding capacity. 

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occured at 8.1 minutes and was calculated as 54 mg/mL using:
(flow rate [2.3 mL/min] X initial protein BSA concentration [0.524 mg/mL] X time [8.1 min]) / membrane bed volume of Mustang Q in 25 mm (1 in.) Acrodisc unit (0.18 mL)

Mustang E Membrane

 

 

Ordering Information

Ordering Information

 
Part Number Description Packaging
 MSTG25KIT  Mustang Starter Kit

4 per package Acrodisc 25 mm (1 in.) with
Mustang Q membrane (MSTG25Q6) 

4 per package Acrodisc 25 mm (1 in.) with
Mustang S membrane (MSTG25S6) 

2 per package Acrodisc 25 mm (1 in.) with
Mustang E membrane (MSTG25E3)

 

Complementary Products

 
Part Number Description Packaging
MSTG25Q6 Acrodisc 25 mm (1 in.) with Mustang Q membrane 10 per package
MSTG25S6 Acrodisc 25 mm (1 in.) with Mustang S membrane 10 per package
MSTG25E3 Acrodisc 25 mm (1 in.) with Mustang E membrane 10 per package
CLM05MSTGQP1 Mustang Q Capsule, 10 mL bed volume  1 per package
CLM05MSTGSP1 Mustang S Capsule, 10 mL bed volume  1 per package
CLM05MSTGEP1 Mustang E Capsule, 10 mL bed volume  1 per package
CL3MSTGQP1

Mustang Q Capsule, 60 mL bed volume  

1 per package
CL3MSTGSP1 Mustang S Capsule, 60 mL bed volume  1 per package
CL3MSTGEP1 Mustang E Capsule, 40 mL bed volume  1 per package
NP6MSTGQP1 Mustang Q Capsule, 260 mL bed volume  1 per package
NP6MSTGSP1 Mustang S Capsule, 260 mL bed volume  1 per package
NP6MSTGEP1 Mustang E Capsule, 160 mL bed volume 1 per package 
NP7MSTGQP1 Mustang Q Capsule, 520 mL bed volume  1 per package
NP7MSTGSP1 Mustang S Capsule, 520 mL bed volume  1 per package
NP8MSTGQP1 Mustang Q Capsule, 780 mL bed volume  1 per package
NP8MSTGSP1 Mustang S Capsule, 780 mL bed volume  1 per package
 
Application
Membranes
Segment
Chromatography