ScreenExpert RoboColumn™ product photo Primary L

ScreenExpert RoboColumn™

For High Throughput Chromatography Sorbent Screening and Optimization

ScreenExpert RoboColumns are miniaturized chromatography columns pre-packed with Pall chromatography sorbents. They are designed for fully automated and parallel chromatographic separations with robotic liquid handling workstations, such as the Freedom EVO from Tecan.

ScreenExpert RoboColumns are packed with Pall anion exchange, mixed-mode,affinity or any other Pall chromatography sorbents upon request. The column units are packed by Atoll GmbH and are identical to Atoll’s MediaScout RoboColumns.

Two formats are available, 200 μL and 600 μL, with a bed height of 10 and 30 mm respectively. The 200 μL column volume is recommended for sorbent and process conditions screening, while the 600 μL is used for purification process optimization or when a residence time above 4 minutes is required (e.g., capacity study on mixed-mode sorbents).

The columns are supplied as rows of eight pre-packed units, with two removable silicon cover seals for proper storage. A 96-well array plate is available to arrange up to 96 ScreenExpert RoboColumn units.

Benefits

  • Column format, and high quality packing enables the ability to mimic all the steps of a chromatography separation in dynamic mode. Data obtained are comparable to those obtained in larger columns
  • Miniaturized format facilitates screening of chromatography sorbents, reduces sample consumption, and saves time
  • Perfectly fitted to Design of Experiment (DoE) requirements, allowing a large amount of experimental work in a minimum of time
  • 96-well format allows easy integration with automation systems for High Throughput Process Development (HTPD)
  • Multiple parameters can be tested in parallel, e.g., residence time
  • Reusable
  • Flexible use: the user makes column selection when needed

Handling

ScreenExpert RoboColumns are designed for robotic handling.

When using the high-throughput process development approach for ScreenExpert RoboColumns, it is suggested to use Design of Experiments (DoE) criteria for the experimental set-up. This enables to screen many different chromatographic parameters such as resin type, pH, conductivity / ionic strength, etc., efficiently and simultaneously.

For experimental set-up and the configuration of the 96-well array plate, the Te-Chrom Wizard is recommended. The Te-Chrom Wizard is a dialog-based graphic user interface offered by Tecan and based on the Freedom EVOware software. The Te-Chrom Wizard was developed for the use of ScreenExpert RoboColumns and provides the possibility to configure hardware, plate layout, and the chromatographic process as well as to set all process relevant chromatographic parameters, e.g., flow rate, volume without direct script writing.

MediaScout and RoboColumn are trademarks of Atoll GmbH. LabChip is a trademark of PerkinElmer. Freedom EVO, Freedom EVOware, and Te-Chrom are trademarks of Tecan Group.


* Required
Need Help?
Download Datasheet

ScreenExpert RoboColumn

List Price
$ 200.00
List Price
$ 200.00
List Price
$ 200.00
List Price
$ 240.00
List Price
$ 240.00
List Price
$ 240.00
List Price
$ 240.00
List Price
$ 200.00
List Price
$ 300.00
List Price
$ 240.00
List Price
$ 360.00

Sorbent

Description

For High Throughput Chromatography Sorbent Screening and Optimization

ScreenExpert RoboColumns are miniaturized chromatography columns pre-packed with Pall chromatography sorbents. They are designed for fully automated and parallel chromatographic separations with robotic liquid handling workstations, such as the Freedom EVO from Tecan.

ScreenExpert RoboColumns are packed with Pall anion exchange, mixed-mode,affinity or any other Pall chromatography sorbents upon request. The column units are packed by Atoll GmbH and are identical to Atoll’s MediaScout RoboColumns.

Two formats are available, 200 μL and 600 μL, with a bed height of 10 and 30 mm respectively. The 200 μL column volume is recommended for sorbent and process conditions screening, while the 600 μL is used for purification process optimization or when a residence time above 4 minutes is required (e.g., capacity study on mixed-mode sorbents).

The columns are supplied as rows of eight pre-packed units, with two removable silicon cover seals for proper storage. A 96-well array plate is available to arrange up to 96 ScreenExpert RoboColumn units.

Benefits

  • Column format, and high quality packing enables the ability to mimic all the steps of a chromatography separation in dynamic mode. Data obtained are comparable to those obtained in larger columns
  • Miniaturized format facilitates screening of chromatography sorbents, reduces sample consumption, and saves time
  • Perfectly fitted to Design of Experiment (DoE) requirements, allowing a large amount of experimental work in a minimum of time
  • 96-well format allows easy integration with automation systems for High Throughput Process Development (HTPD)
  • Multiple parameters can be tested in parallel, e.g., residence time
  • Reusable
  • Flexible use: the user makes column selection when needed

Handling

ScreenExpert RoboColumns are designed for robotic handling.

When using the high-throughput process development approach for ScreenExpert RoboColumns, it is suggested to use Design of Experiments (DoE) criteria for the experimental set-up. This enables to screen many different chromatographic parameters such as resin type, pH, conductivity / ionic strength, etc., efficiently and simultaneously.

For experimental set-up and the configuration of the 96-well array plate, the Te-Chrom Wizard is recommended. The Te-Chrom Wizard is a dialog-based graphic user interface offered by Tecan and based on the Freedom EVOware software. The Te-Chrom Wizard was developed for the use of ScreenExpert RoboColumns and provides the possibility to configure hardware, plate layout, and the chromatographic process as well as to set all process relevant chromatographic parameters, e.g., flow rate, volume without direct script writing.

MediaScout and RoboColumn are trademarks of Atoll GmbH. LabChip is a trademark of PerkinElmer. Freedom EVO, Freedom EVOware, and Te-Chrom are trademarks of Tecan Group.
Applications
ScreenExpert RoboColumns are suitable for a large variety of applications such as:
  • Parallel screening of chromatography sorbents
  • Parallel screening and optimization of chromatographic conditions in downstream process development (conductivity, pH)
  • Scale down experimental work
  • Determination of dynamic binding capacity
  • Determination of the optimal residence time
  • Optimization of the regeneration step

Application Example: Development of the Capture Step of a Monoclonal Antibody (MAb) Expressed in a Crude CHO Feedstock

MAb binding conditions were tested on 3 mixed-mode sorbents and dynamic binding capacity (DBC) was evaluated based on breakthrough (BT) curve (Figure 1).

The ScreenExpert RoboColumn was overloaded with MAb CHO feedstock at 0.52 mg/mL and flowthrough (FT) was collected as one-column-volume (CV) fractions (200 μL each) in 350 μL 96-well plate. The amount of MAb recovered in the FT fractions was evaluated using Bio-Layer Interferometry (BLI) with protein A sensor (Octet® System, Pall ForteBio). It was plotted against the volume of feedstock loaded per ScreenExpert RoboColumn unit. 1/10 of the MAb concentration (C/10) was 0.052 mg/mL (52 μg/mL in Figure 1). The volume equivalent at 10% BT (Veq10%BT) was the volume of MAb loaded when reaching C/10 in FT fractions (8.56 mL in Figure 1).

Figure 1
Breakthrough Curve on MEP HyperCel Sorbent in 200 μL ScreenExpert RoboColumn



Breakthrough curve during the loading of a MAb CHO feedstock (0.52 mg/mL) at a ~4-minute residence time.

DBC10% BT (mg/mL sorbent) =
 
Veq10%BT (mL)* x MAb concentration (mg/mL)
Column volume (mL)
 


*void volume was taken into account

Using this breakthrough curve method, the DBC for MEP, HEA and PPA HyperCel sorbents was determined on 200 μL ScreenExpert RoboColumns (Figure 2).

Figure 2
DBC on 3 Mixed-Mode HyperCel Sorbents in ScreenExpert RoboColumns for Different Binding Conditions


This comparison showed that MAb binding was maximized on MEP HyperCel sorbent in PBS, pH 7.4. The selected condition was then transferred to 1 mL PRC column for confirmation of the DBC, approximately 21 mg/mL (Figure 3).

Figure 3
DBC on MEP HyperCel Sorbent in ScreenExpert RoboColumn and in PRC Column



A 335-fold scale-up of the MAb capture step was finally carried out from 200 μL ScreenExpert RoboColumn to a lab-scale column (2.5 cm I.D) packed with 67 mL MEP HyperCel sorbent (Figure 4).

Figure 4
335-fold Scale-Up of the MAb Capture Step from ScreenExpert RoboColumn to an I.D 25 Lab-Scale Column



Chromatogram of MAb Capture Step on I.D 25 lab-scale column (67 mL). Equilibration in PBS, pH 7.4. Loading of MAb feedstock 21 mg MAb/mL MEP HyperCel sorbent). Residence time = 4 minutes; FT: Flowthrough; W: Wash; E1, E2 and E3: Elution at pH 5, 4 and 3 respectively.

The analysis of fractions obtained on ScreenExpert RoboColumn and the I.D 25 lab-scale column were in the same magnitude. Comparable data in terms of recovery (100%) and purity (90%) were evidenced (Figure 5, Table 2).

Figure 5
Fraction Analysis From ScreenExpert RoboColumn and an I.D 25 Lab-Scale Column Using BLI



Interaction of the MAb with protein A sensor was measured using BLI. Signal is correlated to MAb concentration using a standard curve (data not shown). Residence time = 4 minutes. F0: Sample load; FT: Flowthrough; W: Wash; E1, E2 and E3: Elution at pH 5, 4 and 3 respectively.

Table 2

Summary of the MAb Capture Step on ScreenExpert RoboColumn or an I.D 25 Lab-Scale Column

 
Column
Type
Volume
(mL)
MAb
Loaded
(mg)*
Loading
(mg MAb/
mL sorbent)
Recovery
(%)*
Purity
(%)**
ScreenExpert
RoboColumn
0.2 4 22 102 86
I.D 25 Lab
Scale Column
66.7 1376 21 99 91
 
* Evaluated using BLI technology (Octet System, Pall ForteBio)
** Evaluated using LabChip GXII technology (PerkinElmer)
Specifications
 
ScreenExpert RoboColumns
Column volume 200 μL 600 μL
Bed height 10 mm 30 mm
Column inner diameter 5 mm
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1 to 14
Avoid Halogenated organic solvents, hexane
Storage solution 20% ethanol /1 M NaCl
Recommended storage temperature 2 to 8 °C
Working temperature 4 to 30 °C
Maximum working pressure Up to 8.0 bar g (116 psi g)
Flow rate 16 to 1,000 cm/hr 1
Laser label Placed on every individual column body, and containing:
  • Product number
  • Sorbent name
  • Column volume in μL
96-Well Array Plate
Size 128.3 x 86.0 x 14.0 mm
Material Polyoxymethylene copolymer
Number of wells 96
 
1 The Liquid Transfer menu of the Te-Chrom Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te-Chrom Wizard Software Manual, 396076, en, V1.0).
Ordering Information
 
Description Part Number
ScreenExpert RoboColumn MEP HyperCel 200 μL, row of 8 SR2MEP
ScreenExpert RoboColumn MEP HyperCel 600 μL, row of 8 SR6MEP
ScreenExpert RoboColumn HEA HyperCel 200 μL, row of 8 SR2HEA
ScreenExpert RoboColumn HEA HyperCel 600 μL, row of 8 SR6HEA
ScreenExpert RoboColumn PPA HyperCel 200 μL, row of 8 SR2PPA
ScreenExpert RoboColumn PPA HyperCel 600 μL, row of 8 SR6PPA
ScreenExpert RoboColumn HyperCel STAR AX 200 μL, row of 8 SR2STARAX
ScreenExpert RoboColumn HyperCel STAR AX 600 μL, row of 8 SR6STARAX
ScreenExpert RoboColumn CM Ceramic HyperD F 200 μL, row of 8 SR2CMCHDF
ScreenExpert RoboColumn CM Ceramic HyperD F 600 μL, row of 8 SR6CMCHDF
ScreenExpert RoboColumn KANEKA KanCapA♦ 200 μL, row of 8 SR2KANCAPA
ScreenExpert RoboColumn KANEKA KanCapA 600 μL, row of 8 SR6KANCAPA
ScreenExpert RoboColumn CMM HyperCel 200 µL, row of 8 SR2CMM
ScreenExpert RoboColumn CMM HyperCel 600 µL, row of 8 SR6CMM
96-well RoboColumn array plate SR96WAP
 

Multi-Well Plates (empty)

 
Description Part Number
AcroPrep Advance 96-Well Filter Plate, 1 mL, 0.45 μm Supor® membrane (5/pkg) 8129
 

AcroPrep Advance 96-Well Filter Plate for Ultrafiltration

 
Description Part Number
350 μL, Omega™ 3K MWCO (10/pkg) 8033
350 μL, Omega 10K MWCO (10/pkg) 8034
350 μL, Omega 30K MWCO (10/pkg) 8035
350 μL, Omega 100K MWCO (10/pkg) 8036
 

PRC Prepacked Columns

 
Description Part Number
PRC Column 5x50 MEP HyperCel, 1 mL PRC05X050MEPHCEL01
PRC Column 8x100 MEP HyperCel, 5 mL PRC08X100MEPHCEL01
PRC Column 5x50 HEA HyperCel, 1 mL PRC05X050HEAHCEL01
PRC Column 8x100 HEA HyperCel, 5 mL PRC08X100HEAHCEL01
PRC Column 5x50 PPA HyperCel, 1 mL PRC05X050PPAHCEL01
PRC Column 8x100 PPA HyperCel, 5 mL PRC08X100PPAHCEL01
PRC Column 5x50 HyperCel STAR AX, 1 mL PRCSTARAX1ML
PRC Column 8x100 HyperCel STAR AX, 5 mL PRCSTARAX5ML
PRC Column 5x50 Q Ceramic HyperD F, 1 mL PRC05X050QCHDF01
PRC Column 5x50 CM Ceramic HyperD F, 1 mL PRC05X050CMCHDF01
PRC Column 5x50 KANEKA KanCapA, 1 mL PRC05X050KANCAPA
PRC Column 8x100 KANEKA KanCapA, 5 mL PRC08X100KANCAPA
PRC Column 5x50 CMM HyperCel, 1 mL PRCCMMHCEL1ML
PRC Column 8x100 CMM HyperCel, 5 mL PRCCMMHCEL5ML
 

♦KANEKA KanCapA is a trademark of Kaneka Corporation
Application
Columns - Pre-Packed