Single-pass tangential flow filtration (SPTFF) is revolutionizing how tangential flow filtration (TFF) is implemented in biotech, vaccine, blood plasma
- Ability to couple the concentration of product before or after other downstream processing (DSP) steps, consequently optimizing other steps and reducing in-process pool tank volumes.
- Continuous processing in DSP
- Easy integration of the assembled module, allowing plug and play
- Potential reduction of product damage or aggregation due to reduced residence time and
The preassembled Cadence ILC modules do not need a holder. Once the feed port is connected to a pressurized feed source, the module is ready for use. The built-in resistor in the retentate manifold eliminates the need for downstream instrumentation and allows volume reduction of 2 to 4X (or higher), depending on the application.
Similar to other Cadence SPTFF devices, the Cadence ILC utilizes standard Pall T-Series cassettes as the building blocks for the module. These modules are offered with either Delta regenerated cellulose or Omega polyethersulfone (PES) 10 kDa or 30 kDa membranes providing the high flux, high selectivity and low protein binding characteristics associated with these membranes. They are available in a range of size formats to accommodate various processing volumes.
These products utilize proprietary technology, as well as technology licensed from SPF Innovations, and may be covered by one or more patents. See pall.com/patents.
Optimize Economic and Operational Benefits
Enhance downstream processing to increase capacity and reduce costsCadence ILC modules can help to eliminate or reduce the size of intermediate storage tanks and associated cleaning of tanks when used for in-process volume reduction before or after existing steps.
Enable in-process volume reductionDepending on the initial concentration and product characteristics, a volume reduction of 2 to 4X (or higher) can be achieved with minimal instrumentation or system requirements.
Optimize processing of highly shear-sensitive productsProcessing with the Cadence ILC results in only one pass through the pump and module, reducing shear exposure. For products sensitive to pumping, the pump can be completely eliminated by using pressurized vessels to flow the process fluid through the module. Further benefits are achieved by eliminating any mixing or foaming issues associated with the feed tank.
No holder required
Cadence ILC modules are provided assembled, and the cassettes and manifolds are pre-torqued between two end plates so that no extra holder is required.Benefits include:
- Easy connection to a pressure source, like a pump or pressure vessel
- Simplified in-process volume reduction
- Continuous processing enabled by process coupling
- The module feed, retentate and permeate ports are easily connected to the appropriate outlets using clearly marked ports
Innovative SPTFF processing takes place within the Cadence ILC modules. The modules incorporate Pall’s proven T-series cassettes with Delta or Omega membrane and are available in different formats to accommodate various processing volumes (see ordering information).
Application NotesCleanability and Re-Usability of the Cadence™ Inline Concentrator with Omega™ Membrane
Performance of the Cadence™ Inline Concentrator Modules for the Concentration of Human IgG
Cleanability and Re-usability of Cadence™ Inline Concentrator Modules
Volume Reduction and Process Optimization with Cadence™ Inline Concentrator
Productivity and Economic Advantages of Coupling Single-Pass Tangential Flow Filtration to Multi-Column Chromatography for Continuous Processing
Cadence™ Single-pass TFF Coupled with Chromatography Steps Enables Continuous Bioprocessing while Reducing Processing Times and Volumes
Scalability of Cadence™ Inline Concentrator Modules for Bovine IgG Processing
The Cadence ILC is designed for continuous processing or for in-process volume reduction in various steps in a wide range of applications in the biopharmaceutical industry. These include recovery of antibodies or recombinant proteins, and preparation of samples (concentrating, desalting) before or after column chromatography, and can be implemented in existing facilities facing tank capacity issues or to other process optimization opportunities.
The operating conditions for any single-pass TFF process must be established by performing trials and analyzing results. Pall’s Technical Service Group is available to assist in conducting trials to develop operating conditions necessary to achieve the desired process objectives.
Materials of Construction
|Cassette||Delta regenerated cellulose or Omega PES membrane, 10 kDa or 30 kDa with polypropylene screens, polyurethane encapsulant with white pigment (TiO2), and medical grade silicone for the permeate seals|
|Gaskets||Medical grade, platinum-cured silicone|
|Tubing and connectors||
|Maximum transmembrane pressure (TMP)||4.1
|Processing temperature range/td>||
|pH range||2 – 13|
Typical Feed Flow Rates
|Cadence ILC Module Format||Membrane Area (m2)||QFEED (L/min)|
|T01||0.065||0.05 - 0.3|
|T02||0.13||0.1 - 0.7|
|T12||0.7||0.6 - 3.5|
|T06||3.5||2.9 - 17.5|
|Delta membrane module||< 538 sccm/m2 (<50 sccm/ft2) at 4.1 barg (60 psig)|
|Omega membrane module||<1600 sccm/m2 (<150 sccm/ft2) at 2.1 barg (30 psig)|
Each Cadence module has a unique serial number for full traceability.
The shelf life of Cadence inline concentrator packaged in preservative is 1 year from the date of manufacture when the modules are stored unopened in the original packaging at temperature up to 25 °C and protected from direct light. Extended shelf life studies are ongoing. Please contact Pall for updated information.
The materials of construction for Cadence ILC modules have been tested and meet the requirements for the Biological Reactivity Tests listed in the United States Pharmacopeia (USP) under USP <88> for Class VI - 70 °C Plastics.
DocumentationEach module is supplied with the following comprehensive documentation to ensure the Cadence ILC module is operated successfully. Contact your Pall representative to obtain these documents.
- Certificate of Quality
- Material and safety data sheet (MSDS) for the module preservative solution
- Care and use manual
- Validation Guide
- Application notes
- Validation service for your specific tests such as compatibility testing with your product fluid
- Training and technical support to optimize your process using Cadence ILC modules
- Please contact Pall for further information.
Figure 1Feed flux excursion test results: volume concentration factor (VCF), feed pressure as function of feed flux, 10 g/L human IgG feed solution, T01 (0.065 m2) Cadence ILC module
The range of operating VCF can be determined for a specific feed solution by doing a feed flux excursion.
Ordering InformationIdentify and order Cadence Inline Concentrator using the table below.
Guide to Cadence Inline Concentrator Module Part Numbers
IL Inline concentrator
010 or 030 Nominal molecular weight cut-off (MWCO) in kDa
T01, T02, T12 or T06 Cassette format
04 Number in series
07 Number of cassettes
Cadence Inline Concentrator Part Numbers
|Delta Membrane||Omega Membrane||Cassette Format||Membrane Area (m2)||MKCO (kDa)|