- Medium screen format for clarified solutions, low to medium viscosity, and final concentrations < 30% (300 mg/mL)
- Suspended screen format for:
Solutions containing particles, cells, and cell debris in solution
Solutions that are highly viscous and highly concentrated
Supor polyethersulfone (PES) membrane cassettes are optimized for microfiltration applications, easily scaled and suited to a broad range of cleaning options.
Pall’s tangential flow filtration (TFF) cassettes with Supor membrane are intended for microfiltration (MF) applications. The cassette contains a Supor microfiltration-optimized polyethersulfone membrane.
They are available in two formats – Centramate and Centrasette II cassettes – and in a range of pore sizes from 0.1 μm to 0.65 μm to meet most application requirements.
Proven Supor TFF Membrane
Supor TFF membrane is a modified polyethersulfone membrane with a uniform depth structure. The membrane is optimized for microfiltration applications in TFF including harvest, concentration and clarification of cell cultures and fermentation broths. Supor TFF membranes are ideal for applications including harvesting bacterial, mammalian, and insect cells; separating red blood cells from hemoglobin; and for clarifying yeast broth and bacterial lysates.Features and Benefits
- Low non-specific adsorption due to its hydrophilic chemistry which leads to higher product yields
- Wide range of membrane pore sizes to optimize the process
- Compatible with acids, bases, oxidizing agents and variety of other cleaning agents to fit different application needs
Centramate Microfiltration Cassettes with Supor Membrane
Centramate microfiltration cassettes with Supor membrane are perfect for laboratory scale and development TFF applications. The cassettes are available in a range of channel formats to meet most application requirements. The cassettes are supplied with the same construction materials as used in the Centrasette membrane cassettes.
Applications developed on a Centramate cassette with Supor membrane can be easily scaled up by adding cassettes to a Centramate holder or by switching to the Centrasette cassette format with identical path length and channel format.
Refer to Tables 3 and 4 for a list of membrane and formats available for Centramate cassettes.
Centrasette II Microfiltration Cassettes with Supor Membrane
Centrasette II microfiltration cassettes with Supor membrane, available in the same screen formats as the Centramate cassettes, have been widely used for many years in various process applications including concentration and diafiltration in the biopharmaceutical industry. They are available with membrane areas of 0.5 m2, and 2.5 m2 (5.4 ft2 and 27 ft2). Larger cassette areas simplify installation in large TFF systems, reducing the number of gaskets required, and minimizing risk of leakage.
Pall continues to improve membrane cassettes to meet the more rigid requirements of today’s applications.
The Centrasette II membrane cassette uses the same components as in the original Centrasette cassette. Therefore, revalidation may not be necessary. Feed ports in the Centrasette II cassette have been enlarged to twice the size of the Centrasette cassette and membrane area has been increased by about 10%. The larger fluid port design will enhance the membranes performance by:
- Improving fluid flow distribution between and through cassettes
- Allowing more efficient cleaning
- Reducing the pressure profile across the feed/retentate channels
Centrasette (left) and Centrasette II (right) cassettes feed port comparison
The Centrasette II microfiltration cassettes with Supor membrane are available with 2 different membrane area options to meet small scale and larger process volume requirements (see Table 4).
Cassette Channel Configurations
Pall’s microfiltration cassettes with Supor Membrane are available in medium and suspended screen channel formats to best meet the specific requirements of each application.
Table 1 - Selection criteria for a cassette channel configuration
|Channel Configuration||Selection Criteria|
|Medium screen||Clarified solutions; low – medium viscosity
Final concentration < 30% (300 mg/mL)
|Suspended screen / open channel||Particles/cells/cell debris in solution
Table 2 - Materials of construction
|Membrane||Supor microfiltration-optimized polyethersulfone|
Cassettes pass current USP Biological Safety Tests for Class VI Plastics at 70 °C.
Pall’s microfiltration cassettes with Supor Membrane are shipped wet, in liquid containing a humectant and bactericidal storage solution. This solution consists of 15 – 20% glycerin, and 0.05 – 0.1% sodium azide.
The storage solution must be removed and the cassette flushed well with water prior to use to prevent product contamination.
Each microfiltration cassette with Supor membrane has a unique serial number for full traceability.
Each cassette is supplied with:
- Certificate of quality
- Membrane cassette care and use procedures
- Material safety data sheet (MSDS) for cassette preservative
- Two platinum-cured silicon gaskets
Cassette holders are available, please contact Pall for details.
- Membrane type is PS (Supor TFF microfiltration-optimized polyethersulfone)
- Choose a μm rating code from Table 3 (e.g., M20 = 0.2 μm).
- Choose a format code from Table 4 (e.g., C11 or F06).
Typical example of part number: PS M20 C11
Table 3 – Available micron ratings for microfiltration Supor membranes
|Code||Pore Size (μm)|
Table 4 – Cassette and screen formats
|Cassette Format||Nominal Area||Medium Screen Code||Suspended Screen Code|
|Centramate||0.01 m2 (0.1 ft2)||C12P1||C11P1|
|0.02 m2 (0.2 ft2)||C12P2||C11P2|
|0.1 m2 (1.0 ft2)||C12||C11|
|Centrasette II||0.5 m2 (5.4 ft2)||F06||F07|
|2.5 m2 (27 ft2)||F26||F27|