T-Series TFF Cassettes - Delta product photo Primary L

T-Series TFF Cassettes - Delta

Low Protein Binding Delta Membrane Used in the Proven T-Series Cassette Design Optimizes Process Economics


  • Improved process performance. Cassettes are designed to provide optimal mass transfer to improve your process economics.
  • Increased safety, reliability, and reproducibility. Superior new materials of construction are durable and stable, and exhibit very low extractables. Membrane is backpressure resistant up to 4.1 barg (60 psig).
  • Easy scale up for robust purification processes. Available in scalable formats with the same materials of construction from development to production-scale processes.  
  • High flux, high selectivity, and low protein binding are achieved through the use of Delta regenerated cellulose membrane.

Delta Regenerated Cellulose Membrane

Delta regenerated cellulose membrane offers high flux and selectivity. The membrane has been specifically developed to minimize protein binding to the surface and interstitial structure of the membrane. Inherently hydrophilic, this membrane shows low protein adsorption properties and is optimal for processes involving very hydrophilic proteins. Delta regenerated cellulose membrane shows low fouling characteristics, allowing a constant performance or flux during a production run. It is easy to clean and typically recovers normalized water permeability (NWP) by using only 0.8N saline solution.

Figure 1

T-Series Cassettes with Delta Membrane Offer Excellent Scalability Among Formats
 
T01 93 cm2 (0.1 ft2) EFA
T02 186 cm2 (0.2 ft2) EFA
T12 0.1 m2 (1.1 ft2) EFA
T06 0.5 m2 (5.4 ft2) EFA
T26 2.5 m2 (27 ft2) EFA
 

The T-Series Advantage

Superior new materials of construction and improved cassette design increase process safety, reliability, reproducibility, and productivity:
  • The feed and permeate screen material for T-Series cassettes is made from polypropylene which is highly resistant to sodium hydroxide.
  • Larger feed and permeate ports provide lower pressure drops.
  • T-Series cassettes have been designed to provide maximum mass transfer through the membrane, resulting in faster processing times or reduced area installations compared to existing similar cassette formats.
To test the new T-Series cassettes with Delta membrane in your process, contact Pall today.


* Required

Cassettes

NMWC 10 kD, Centramate 93 cm² (0.1 ft²)
List Price
$ 340.00
NMWC 10 kD, Centramate 186 cm² (0.2 ft²)
List Price
$ 421.00
NMWC 10 kD, Centramate 0.1 m2 (1.1 ft²)
List Price
$ 822.00
NMWC 30 kD, Centramate 93 cm² (0.1 ft²)
List Price
$ 340.00
NMWC 30 kD, Centramate 186 cm² (0.2 ft²)
List Price
$ 421.00
NMWC 30 kD, Centramate 0.1 m2 (1.1 ft²)
List Price
$ 822.00
Description

Low Protein Binding Delta Membrane Used in the Proven T-Series Cassette Design Optimizes Process Economics


  • Improved process performance. Cassettes are designed to provide optimal mass transfer to improve your process economics.
  • Increased safety, reliability, and reproducibility. Superior new materials of construction are durable and stable, and exhibit very low extractables. Membrane is backpressure resistant up to 4.1 barg (60 psig).
  • Easy scale up for robust purification processes. Available in scalable formats with the same materials of construction from development to production-scale processes.  
  • High flux, high selectivity, and low protein binding are achieved through the use of Delta regenerated cellulose membrane.

Delta Regenerated Cellulose Membrane

Delta regenerated cellulose membrane offers high flux and selectivity. The membrane has been specifically developed to minimize protein binding to the surface and interstitial structure of the membrane. Inherently hydrophilic, this membrane shows low protein adsorption properties and is optimal for processes involving very hydrophilic proteins. Delta regenerated cellulose membrane shows low fouling characteristics, allowing a constant performance or flux during a production run. It is easy to clean and typically recovers normalized water permeability (NWP) by using only 0.8N saline solution.

Figure 1

T-Series Cassettes with Delta Membrane Offer Excellent Scalability Among Formats
 
T01 93 cm2 (0.1 ft2) EFA
T02 186 cm2 (0.2 ft2) EFA
T12 0.1 m2 (1.1 ft2) EFA
T06 0.5 m2 (5.4 ft2) EFA
T26 2.5 m2 (27 ft2) EFA
 

The T-Series Advantage

Superior new materials of construction and improved cassette design increase process safety, reliability, reproducibility, and productivity:
  • The feed and permeate screen material for T-Series cassettes is made from polypropylene which is highly resistant to sodium hydroxide.
  • Larger feed and permeate ports provide lower pressure drops.
  • T-Series cassettes have been designed to provide maximum mass transfer through the membrane, resulting in faster processing times or reduced area installations compared to existing similar cassette formats.
To test the new T-Series cassettes with Delta membrane in your process, contact Pall today.
Applications
T-Series cassettes with Delta regenerated cellulose membrane are designed for development, pilot, and production-scale TFF applications in diverse biological and biopharmaceutical processes. They are especially useful in:
  • Antibody fragments (Fab)
  • Purification and recovery of antibodies 
  • Concentration and diafiltration of recombinant proteins
  • Blood plasma fractionation and purification

Product Platforms

T-Series cassettes complement Pall’s highly successful Centramate™ and Centrasette™ products, and have been developed following years of experience designing and optimizing our customers’ biotech and biopharmaceutical processes.

T-Series cassettes for Centramate holders are offered in scalable membrane formats with effective filtration areas (EFA) from as low as 93 cm2 (0.1 ft2), making them ideal for process development and small-scale production of 50 mL to 125 L. Centrasette T-Series cassettes, in combination with Centrasette holders, can process thousands of liters with installations incorporating hundreds of square meters of EFA.
Specifications

Materials of Contruction

 
Membrane Delta regenerated cellulose
Support Polypropylene
Screens Polypropylene 
Encapsulent Polyurethane with white pigment (TiO2)
Permeate Seals Medical grade, platinum cured silicone
Gaskets Medical grade, platinum cured silicone
 

Operating Limits

 
Maximum Pressure1 6 barg (90 psig) @ 23 °C
4 barg (60 psig) @ 55 °C
Maximum TMP 4 barg (60 psig) @ 55 °C
Temperature Range2 4 – 55 °C
pH Range 2 – 13
 
1 Pressure rating will be dependant on rating of the lowest system component.
2 Cassettes must not be allowed to freeze.

Typical Operating Parameters

 
Cross Flow Rate for Processing 5 – 7 L/min/m2 (0.5 – 0.7 L/min/ft2)
Cross Flow Rate for Cleaning 5 – 8 L/min/m2 (0.5 – 0.8 L/min/ft2)
 

Integrity Test

 
Test Pressure 4 barg (60 psig)
Maximum Air Forward Flow < 500 sccm/m2 (< 50 sccm/ft2)
 

Shelf Life

The shelf life of cassettes packaged in preservative is expected to be three years from the date of manufacture when the cassettes are stored unopened in the original packaging at 4 – 25 °C and protected from direct light. Extended shelf life studies are ongoing. 

Biological Safety

All of the materials of construction in the T-Series cassettes have been tested and meet requirements for United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo
<88> at 70 °C.
Ordering Information
T-Series Cassettes with Delta Low Protein Binding, Regenerated Cellulose Membrane
 
Part Number NMWC* Type and Nominal Membrane Area
DC010T01 10 kD Centramate 93 cm2 (0.1 ft2)
DC010T02 10 kD Centramate 186 cm2 (0.2 ft2)
DC010T12 10 kD Centramate 0.1 m2 (1.1 ft2)
DC010T06 10 kD Centrasette 0.5 m2 (5.4 ft2)
DC010T26 10 kD Centrasette 2.5 m2 (27 ft2)
DC030T01 30 kD Centramate 93 cm2 (0.1 ft2)
DC030T02 30 kD Centramate 186 cm2 (0.2 ft2)
DC030T12 30 kD Centramate 0.1 m2 (1.1 ft2)
DC030T06 30 kD Centrasette 0.5 m2 (5.4 ft2)
DC030T26 30 kD Centrasette 2.5 m2 (27 ft2)
 

*NMWC = Nominal Molecular Weight Cutoff
Application
Cassettes
Segment
Tangential Flow Filtration