Beer is quite restrictive to bacterial growth due to its characteristics (low pH, ethanol concentration, low oxygen content), but a few bacterial genera, including Lactobacillus, Pediococcus, Pectinatus, and Megasphaera, can generate off-flavors, turbidity and acidity. Related bacterial beer spoilage can generate high economic losses and impact brand image.
Furthermore, labor intensive and lengthy investigations after spoilage detection can increase these risks as spoilage can originate from a wide variety of sources throughout the brewing process. These investigations can generate increased losses, either from continued production of spoiled product or product interruptions.Pall GeneDisc solutions help brewers reduce these risks by enabling the following:
- Implementation of Decision making tests at key control points of the process
- Fast investigations after spoilage detection
GeneDisc System Benefits
Rapid — Pall's GeneDisc method allows a simultaneous detection and identification of beer spoilage bacteria in a few days and colony identification in 2 hours.
Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.
Modular — Scalable PCR system with 1 to 8 units adapts to evolving testing needs.
A Solution Designed for BreweriesPall offers two methods for spoilage monitoring in beer samples depending on the brewery requirements:
- a sensitive method including an enrichment step to guarantee absence of spoiler in the sample
- a very fast method enabling monitoring of a low level of spoilers in less than 3 hours
Pall’s GeneDisc method for beer spoilers can simultaneous detect and identify 24 of the major beer spoiling microorganisms including:
- Lactobacillus brevis
- Lactobacillus lindneri
- Lactobacillus backii
- Lactobacillus collinoides and L.paracollinoides
- Lactobacillus group: L.casei, L. paracasei, L.coryniformis, L. rossiae, L.parabuchneri (= frigidus), L.perolens and L.plantarum
- Pediococcus (P. damnosus, P. inopinatus, P. claussenii)
- Pectinatus (P. cerevisiiphillus, P. frisingensis, P. haikarae, P. portalensis)
- Megasphaera spp. (M. cerevisiae, M. elsdenii)
Reliable results – To ensure result accuracy, each sample analysis includes an internal positive control. In addition, results from internal and external studies demonstrated the method was able to accurately detect the presence of beer spoilage bacteria even at low contamination levels in beer samples.
Easy to use – GeneDisc method allows clear beer spoilage bacteria detection and identification from various sample types including in-process and final product samples.
Beer Spoilage Bacteria ID
|Bacteria Genus||Description||Can Generate Turbidity||Main Associated Off-flavors|
|Lactobacillus||Gram + bacilli||Yes||Lactic acids|
|Pediococcus||Gram + cocci||Yes||Diacetyl|
|Pectinatus||Firmicutes||Yes||Sulphur compounds (e.g. H2S)|
|Megasphaera||Firmicutes||Yes||Valeric and isovaleric acids|
How the System Works
|Enrichment Time||With common broths (e.g. MRS or NBB). (The enrichment step is not required for the direct monitoring method.)|
|Sample Preparation Time||< 1 hour for 48 samples with Extraction Pack Food 1|
|Hands-on Time||About 45 minutes for 48 samples ( < 1 min/sample)|
|PCR Cycle Time||< 1 hour|
|Limit of Detection
|Internal Positive Control||Ensure PCR reaction is not affected by the presence of inhibitors for each sample DNA extract|
- Fast and Reliable Detection of Beer Spoilage Bacteria for Routine Analysis – Field Results, Ziehl J. et al., Pall Corporation and BITBURGER BRAUGRUPPE GmbH.
- Practical Application of qPCR for Monitoring and Improving Brewery Sanitation, Fyfe L. et al., Craft Brew Alliance and Pall Corporation.
- Performance Evaluation of the GeneDisc Method for Detection of Beer Spoilage Bacteria, Bonilla S. et al., Pall Corporation.
- Implementation of Real-time PCR to Ensure Bacteria Free Yeast Propagations in a Mid-sized Craft Brewery, Bailey B., Tröegs Brewing Co.
|EGDCV3A||GeneDisc Cycler Base Unit||-|
|EGDSV3A||GeneDisc Cycler Sub Unit||-|
|PFOOD1100||Extraction Pack Food 1||100|
|GeneDisc Beer Spoilage Bacteria||36|
We also offer a full product range for pathogen detection in food and water as well as detection and identification of Alicyclobacillus in beverages.
Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.