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GeneDisc® Technologies - Yeast Detection and Identification

For the rapid and flexible detection and identification of spoilage yeast in beverages

The role of yeast within fermentation is central for the production of fermented alcoholic beverages. In addition to this beneficial role, yeasts can also cause spoilage to alcoholic beverages both during and after fermentation.

Spoilage yeast growth can affect the chemical composition of the beverage, generating off flavors, undesirable aroma or altered appearance. It can also lead to broken packaging due to increasing pressure. Such spoilage can generate high economic losses and impact brand image.

Labor intensive and lengthy investigations after spoilage detection can generate further spoilage risks as origination can come from a wide variety of sources throughout the production process. Long investigations can increase losses as higher volume of spoiled product could be produced or production could be interrupted for a long period of time.

Pall GeneDisc Technologies help breweries to reduce these risks with solutions enabling the following:
  • Implementation of rapid decision making tests for product release and key control points of the process
  • Fast investigations after spoilage detection

GeneDisc System Benefits

Rapid — Pall’s GeneDisc method allows detection and identification of spoilage yeast via direct monitoring and Brettanomyces quantification in as fast as 2 hours. For methods requiring enrichment results can be available in 30 hours as compared to 5 to 7 days with culture method.

Flexible — Analysis with enrichment ensures high sensitivity (as low as 1 cell/sample) when precise information is the priority, while direct monitoring offers low level detection when faster time to result is the driver.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

Modular — Screening and identification solutions allow testing on various sample types.

Solutions Designed for Beverage Industry

Pall’s solution for yeast provides customers with 2 GeneDisc plates for different testing option:

 
GeneDisc Plate for Yeast Screening GeneDisc Plate for Yeast ID
Screens for yeast Detects and identifies 12 major spoilage yeast strains and species simultaneously
For testing yeast-free samples (e.g.bottled product, raw materials,soft drinks) For assessing risk associated with spoilage detected using the GeneDisc Plate for Yeast Screening For testing wild spoilage yeast in samples containing process yeast
For Brettanomyces quantification in wine samples.
 

Reduced hands-on cost – Ease of use simplifies testing workflows and on-site implementation.

Spoilage Yeast ID

 
Major Spoilage Yeast
  • Saccharomyces spp. (including S. cerevisiae, S.pastorianus, S. cerevisiae var. diastaticus)
  • Brettanomyces / Dekkera spp. ( including B. /D. bruxellensis )
  • Candida spp. / Pichia spp.
  • Zygosaccharomyces spp. (including Z. bailii )
  • Saccharomycodes spp.
  • Schizosaccharomyces spp.
Organoleptic Impact
  • Off-flavors and sensory defects (e.g. acetic acid, phenolic compounds, esters, acetaldehyde)
  • Film formation
  • Turbidity
  • Sediment
  • Unwanted alcoholic fermentation
Effect on Process
  • Decreased filterability
  • Exploding bottles or bulging cans
 


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Description

For the rapid and flexible detection and identification of spoilage yeast in beverages

The role of yeast within fermentation is central for the production of fermented alcoholic beverages. In addition to this beneficial role, yeasts can also cause spoilage to alcoholic beverages both during and after fermentation.

Spoilage yeast growth can affect the chemical composition of the beverage, generating off flavors, undesirable aroma or altered appearance. It can also lead to broken packaging due to increasing pressure. Such spoilage can generate high economic losses and impact brand image.

Labor intensive and lengthy investigations after spoilage detection can generate further spoilage risks as origination can come from a wide variety of sources throughout the production process. Long investigations can increase losses as higher volume of spoiled product could be produced or production could be interrupted for a long period of time.

Pall GeneDisc Technologies help breweries to reduce these risks with solutions enabling the following:
  • Implementation of rapid decision making tests for product release and key control points of the process
  • Fast investigations after spoilage detection

GeneDisc System Benefits

Rapid — Pall’s GeneDisc method allows detection and identification of spoilage yeast via direct monitoring and Brettanomyces quantification in as fast as 2 hours. For methods requiring enrichment results can be available in 30 hours as compared to 5 to 7 days with culture method.

Flexible — Analysis with enrichment ensures high sensitivity (as low as 1 cell/sample) when precise information is the priority, while direct monitoring offers low level detection when faster time to result is the driver.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

Modular — Screening and identification solutions allow testing on various sample types.

Solutions Designed for Beverage Industry

Pall’s solution for yeast provides customers with 2 GeneDisc plates for different testing option:

 
GeneDisc Plate for Yeast Screening GeneDisc Plate for Yeast ID
Screens for yeast Detects and identifies 12 major spoilage yeast strains and species simultaneously
For testing yeast-free samples (e.g.bottled product, raw materials,soft drinks) For assessing risk associated with spoilage detected using the GeneDisc Plate for Yeast Screening For testing wild spoilage yeast in samples containing process yeast
For Brettanomyces quantification in wine samples.
 

Reduced hands-on cost – Ease of use simplifies testing workflows and on-site implementation.

Spoilage Yeast ID

 
Major Spoilage Yeast
  • Saccharomyces spp. (including S. cerevisiae, S.pastorianus, S. cerevisiae var. diastaticus)
  • Brettanomyces / Dekkera spp. ( including B. /D. bruxellensis )
  • Candida spp. / Pichia spp.
  • Zygosaccharomyces spp. (including Z. bailii )
  • Saccharomycodes spp.
  • Schizosaccharomyces spp.
Organoleptic Impact
  • Off-flavors and sensory defects (e.g. acetic acid, phenolic compounds, esters, acetaldehyde)
  • Film formation
  • Turbidity
  • Sediment
  • Unwanted alcoholic fermentation
Effect on Process
  • Decreased filterability
  • Exploding bottles or bulging cans
 
Specifications

How the System Works

Technical Information

 
Enrichment Time Down to 28 h - Optional
Sample Preparation Time < 1 hour for 48 samples
Hands-on Time About 45 minutes for 48 samples ( < 1 min/sample)
PCR Cycle Time About 1 hour
Total Turnaround Time
  • Down to 30 h for detection
  • Down to 2 h for direct monitoring, Brettanomyces quantification and colony identification.
GeneDisc Plate for Yeast ID – Targets
  • Saccharomyces spp.
  • Saccharomyces cerevisiae
  • Saccharomyces pastorianus
  • Saccharomyces cerevisiae var. diastaticus
  • Brettanomyces / Dekkera spp.
  • Brettanomyces / Dekkera bruxellensis
  • Candida spp. / Pichia spp.
  • Zygosaccharomyces spp.
  • Zygosaccharomyces bailii
  • Saccharomycodes spp.
  • Schizosaccharomyces spp.
Limit of Detection (before enrichment) Down to 1 cell/sample before enrichment.
Limit of Detection (direct monitoring) Down to 1 cell/mL
Compatible with
  • Filterable samples
  • Unfilterable samples
  • Yeast slurry and fermenter
Internal Positive Control Ensure PCR reaction is not affected by the presence of inhibitors for each sample DNA extract
 
Type
Testing and Monitoring
Use
Monitoring, Testing
Ordering Information
 
Part Number Description Samples/pack
  Equipment
EGDUL1A230 (EU)
EGDUL1A120 (US)
or
EGDBH48230 (EU)
EGDBH48120 (US)
GeneDisc Cycler Ultra-Lyser
GeneDisc DryBlock Heater 48
EGDCV3A GeneDisc Cycler Base Unit  
EGDSV3A GeneDisc Cycler Sub Unit
 
  Consumables
PFOOD1100 Extraction Pack Food 1 100
GYEASCR106006 GeneDisc Plate for Yeast Screening 36
GYEASCR112006 72
GYEASID103012 GeneDisc Plate for Yeast ID 36
GYEASID106006 36
 

We also offer a full product range for pathogen detection in food and water as well as detection and identification of spoilage bacteria in beverages (Beer spoilage bacteria, TAB spoilage).

Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus) are also available.

For more information including part numbers please contact us.

Application
QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, QC/Microbial Detection - Spoilage, Beverage Spoilage, Microcontrol with PCR, Microcontrol with PCR, Microcontrol with PCR, Microcontrol with PCR, Microcontrol with PCR, Microcontrol with PCR, Yeast Screening & Identification, Yeast Screeing and Identification, Laboratory Analysis, Yeast Screening & Identification