GeneDisc® Technologies - STEC product photo Primary L

GeneDisc® Technologies - STEC

A Flexible Approach for the Detection of Top 5 and Top 7 Shiga Toxic E. coli

Escherichia coli are common in human bacterial flora. Most of them are harmless, but some known as Shiga Toxic E. coli or STEC, can cause severe, sometimes lethal, pathologies.

Main source of infection is contaminated food making it a major concern for food processors.

Among these STEC, several serogroups are more commonly related to human diseases. In Europe, STEC Top 5 serogroups include E. coliO157, O26, O103, O111 and O145. In the USA, serogroups O45 and O121 are added to this list making the STEC Top 7.

GeneDisc System Benefits

Rapid — Accelerate the batch release of your short shelf life products and raw materials. While other methods such as immunoassays or culture methods require up to 3 days to results, the Pall GeneDisc method allows a detection of pathogenic STEC in as fast as 10 hours.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

High throughput capability — Process up to 96 samples DNA extractions simultaneously in less than one hour.

Modular — System modularity fits your throughput needs: up to 96 samples can be analyzed in a one hour PCR run.


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Description

A Flexible Approach for the Detection of Top 5 and Top 7 Shiga Toxic E. coli

Escherichia coli are common in human bacterial flora. Most of them are harmless, but some known as Shiga Toxic E. coli or STEC, can cause severe, sometimes lethal, pathologies.

Main source of infection is contaminated food making it a major concern for food processors.

Among these STEC, several serogroups are more commonly related to human diseases. In Europe, STEC Top 5 serogroups include E. coliO157, O26, O103, O111 and O145. In the USA, serogroups O45 and O121 are added to this list making the STEC Top 7.

GeneDisc System Benefits

Rapid — Accelerate the batch release of your short shelf life products and raw materials. While other methods such as immunoassays or culture methods require up to 3 days to results, the Pall GeneDisc method allows a detection of pathogenic STEC in as fast as 10 hours.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

High throughput capability — Process up to 96 samples DNA extractions simultaneously in less than one hour.

Modular — System modularity fits your throughput needs: up to 96 samples can be analyzed in a one hour PCR run.
Specifications
 

Choose Your GeneDisc Solutions

Pall GeneDisc Technologies provide you with flexible solutions matching your needs for a reliable monitoring of STEC.

 
Follow reference method Reduce your rate of presumptive positive
In line with MLG 5B and ISO/TS 13136 – With this method, a systematic screening based on virulence factors allows to discriminate pathogenic strains from non pathogenic ones. If result is positive, an identification of the Top 7 or Top 5 serogroups is performed.

Test Salmonella spp. simultaneously – Analysis with Salmonella spp. is available and does not require any additional hands-on time nor enrichment.
Enhanced workflow – This method enables to reduce the number of presumptive positive sample using a cutting edge approach. With this method, all targets – serogroups and virulence factors – are analyzed within one GeneDisc plate.

High level of discrimination – An accurate virulence factor screening based on the association of these factors to serogroups provides a lower rate of presumptive positive than any other available method.
 

Shiga Toxic E. coli Identification

 
Bacteria Gram –, motile enterobacteria producing shigatoxins (stx) and intimin (eae)
Food Vehicle Large variety of food especially beef cattle and dairy products
Disease Gastro-enteritis, Hemorrhagic diarrhea, Hemolytic-uremic syndrome (HUS)
Incidence of Cases
(per 100,000 population)
1.90 (FoodNet, 2010)
Notification Rate
(per 100,000 population)
0.83 (EFSA, ECDC, 2010)
Related Outbreaks 2 (EFSA, ECDC, 2010)
25 (CDC, 2010)
Related Recalls 8 (FDA & FSIS, 2012)
Related Alerts / Information 22 (RASFF, 2011)
 

Validations

 
Method Part Number Validation Matrix Time to Result
(Down to)
STEC GSTEHEC1XX006 AOAC-PTM Raw Ground Beef/
Raw Beef Trim
 
STEC &
Salmonella spp.
GSTECSL2XX006  
STEC Top 7 GTOP7EC106006
GSTECPL206006*
 
E. coli O157:H7 GSTEHEC1XX006
GEHECID106006
NF
VALIDATION
Raw Beef Meat 10 h
Dairy products/
Vegetables
products
18 h
E. coli O157:H7 & Salmonella spp. GSTECSL2XX006
GEHECID106006
Raw Beef Meat 10 h
Dairy products/
Vegetables
products
18 h
 
All methods are approved by AOAC using both Extraction Pack Food 1 and Food 2

* Confirmation tool for the AOAC-PTM certified GeneDisc STEC Top 7 method

Technical Information

 
Enrichment Time Down to 8 hours
Sample Preparation Time < 1 hour for 96 samples
PCR Cycle Time < 1 hour
Total Turnaround Time Down to 10 hours
Limit Of Detection 1 bacteria in 25 g of food sample
1 bacteria in 375 g of raw ground beef or raw beef trim
Specificity Wide range of strains tested for inclusivity and exclusivity
Internal Positive Control Per Sample Analysis Detects presence of inhibitors in each sample DNA extract
 
Type
Testing and Monitoring
Use
Monitoring, Testing
Ordering Information
 
Part Number Description Samples/pack
Equipment
EGDCV3A GeneDisc Cycler Base Unit -
EGDSV3A GeneDisc Cycler Sub Unit -
EGDUL1A230 (EU)
EGDUL1A120 (US)
GeneDisc Ultra-Lyser -
OR
EGDBH96230 (EU)
EGDBH96120 (US)
GeneDisc DryBlock Heater 96 -
SPSKIT96 Extraction Pack Food 2 Starter Kit -
 
Consumables
PFOOD1100 Extraction Pack Food 1 100
PFOOD2096 Extraction Pack Food 2 (High throughput) 96
GSTEHEC106006
GSTEHEC112006
GeneDisc ShigaToxic E. coli* 36
72
GSTECSL206006
GSTECSL212006
GeneDisc ShigaToxic E. coli* & Salmonella spp. 36
72
GEHECID106006 GeneDisc EHEC 5 ID (H7, O26, O103, O111, O145) 36
GTOP7EC106006 GeneDisc STEC Top 7 (virulence genes and O157, O26, O103, O111, O145, O45, O121 serogroups) 36
GSTECPL206006 GeneDisc STEC Plus (virulence genes) 36
 
*Includes identification of pathogenic E. coli O157


We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage. Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus...) are also available. For more information including part numbers please contact us.
Application
QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, Food Safety