GeneDisc® Technologies - E. coli O157(:H7) product photo Primary L

GeneDisc® Technologies - E. coli O157(:H7)

For easy, rapid and reliable detection of pathogenic E. coli O157(:H7) in food

Escherichia coli are common in human bacterial flora. Most of them are harmless, but some known as Shiga Toxic E. coli or STEC, can cause severe, sometimes lethal, pathologies.

Among these STEC, E. coli O157:H7 is the most commonly reported serotype related to human diseases. Main infection source is contaminated food making it a major concern for food processors.

Pall GeneDisc Technologies provides an ideal solution for food companies in need of a reliable control of E. coliO157(:H7) risk.

GeneDisc System Benefits

Rapid — Accelerate the batch release of your short shelf life products and raw materials. While other methods such as immunoassays or culture methods require up to 3 days to results, the Pall's GeneDisc method allows a detection of pathogenic E. coli O157 in as fast as 10 hours.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

High throughput capability — Process up to 96 samples DNA extractions simultaneously in less than one hour.

Modular — System modularity fits your throughput needs: up to 96 samples can be analyzed in a one hour PCR run.


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Order Products

GeneDisc® E. Coli O157, GECO157106006

Product ID: GECO157106006
Unit of Measure
1/PAC
Min Order Qty
1
Number of Samples per GeneDisc Plate
6
Number of Samples per Kit
36

GeneDisc® E. Coli O157/Salmon 06, GECOSLM206006

Product ID: GECOSLM206006
Unit of Measure
1/PAC
Min Order Qty
1
Number of Samples per GeneDisc Plate
6
Number of Samples per Kit
36

GeneDisc® E. Coli O157/Salmon 12, GECOSLM212006

Product ID: GECOSLM212006
Unit of Measure
1/PAC
Min Order Qty
1
Number of Samples per GeneDisc Plate
12
Number of Samples per Kit
72

Documents

Datasheets

Download: English

Description

For easy, rapid and reliable detection of pathogenic E. coli O157(:H7) in food

Escherichia coli are common in human bacterial flora. Most of them are harmless, but some known as Shiga Toxic E. coli or STEC, can cause severe, sometimes lethal, pathologies.

Among these STEC, E. coli O157:H7 is the most commonly reported serotype related to human diseases. Main infection source is contaminated food making it a major concern for food processors.

Pall GeneDisc Technologies provides an ideal solution for food companies in need of a reliable control of E. coliO157(:H7) risk.

GeneDisc System Benefits

Rapid — Accelerate the batch release of your short shelf life products and raw materials. While other methods such as immunoassays or culture methods require up to 3 days to results, the Pall's GeneDisc method allows a detection of pathogenic E. coli O157 in as fast as 10 hours.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

High throughput capability — Process up to 96 samples DNA extractions simultaneously in less than one hour.

Modular — System modularity fits your throughput needs: up to 96 samples can be analyzed in a one hour PCR run.

Specifications

A Solution Designed for Food Industries

Pathogenic strains discrimination – Screening method based on detection of virulence factors (shigatoxin and intimin) provides a lower rate of presumptive positive.

In line with MLG 5 and ISO 16654 – NF VALIDATION and AOAC certified method.

Test Salmonella spp. simultaneously –Analysis with Salmonella spp. is available and does not require any additional hands-on time nor enrichment.

Pathogenic E. coli O157 Identification

 
Bacteria Gram –, motile enterobacteria producing shigatoxins (stx) and intimin (eae)
Food vehicle Large variety of food especially dairy products and beef cattle
Disease Gastro-enteritis, Hemorrhagic diarrhea, Hemolytic-uremic syndrome (HUS)
Incidence of cases (per 100,000 population) 0.95 (FoodNet, 2010)
Notification rate (per 100,000 population) 0.32 (EFSA, ECDC, 2010)
Related outbreaks Not determined (Europe)
53 (CDC, 2009-2010)
Related recalls 8 (FDA & FSIS, 2012)
Related alerts/information 8 (RASFF, 2011)
 


Performance

How the System Works

Technical Information

 
Enrichment Time Down to 8 hours
Sample Preparation Time < 1 hour for 96 samples
PCR Cycle Time < 1 hour
Total Turnaround Time Down to 10 hours
Hands On Time About 30 minutes for 96 samples (<30 s/sample)
Limit Of Detection 1 bacteria in 25 g of food sample
1 bacteria in 375 g of raw ground beef or raw beef trim
Specificity Wide range of strains tested for inclusivity and exclusivity
Internal Positive Control Per Sample Analysis Detects presence of inhibitors in each sample DNA extract
 

Validations

 
Method Part Number Validation Matrix Time to Result
E. coli O157 GECO1571XX006 AOAC Raw Ground Beef/
Raw Beef Trim
10 h
E. coli O157 &
Salmonella spp.
GECOSLM2XX006
E. coli O157:H7 GECO1571XX006
GEHECID106006
NF
VALIDATION
Raw Beef Meat 10 h
Dairy products/
Vegetables products
18 h
E. coli O157:H7
& Salmonella spp.
GECOSLM2XX006
GEHECID106006
Raw Beef Meat 10 h
Dairy products/
Vegetables products
18 h
 

Type

GeneDisc

Use

Diagnostics, Monitoring, Testing

Ordering Information

 
Part Number Description Samples/pack
Equipment
EGDCV3A GeneDisc Cycler Base Unit -
EGDSV3A GeneDisc Cycler Sub Unit -
EGDUL1A230 (EU)
EGDUL1A120 (US)
GeneDisc Ultra-Lyser -
or
EGDBH96230 (EU)
EGDBH96120 (US)
GeneDisc DryBlock Heater 96 -
SPSKIT96 Extraction Pack Food 2 Starter Kit -
 
Consumables
PFOOD1100 Extraction Pack Food 1 100
FOOD2096 Extraction Pack Food 2 (High throughput) 96
GECO157106006
GECO157112006
GeneDisc Pathogenic E. coli O157 36
72
GECOSLM206006
GECOSLM212006
GeneDisc Pathogenic E. coli O157 & Salmonella spp. 36
72
GEHECID106006 GeneDisc EHEC 5 ID (H7, O26, O103, O111, O145) 36
 


We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage.

Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus...) are also available.

For more information including part numbers please contact us.

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