For the rapid and flexible detection and identification of spoilage yeast in beverages
The role of yeast within fermentation is central for the production of fermented alcoholic beverages. In addition to this beneficial role, yeasts are also able to cause spoilage to alcoholic beverages both during and after fermentation.
Spoilage yeast growth can affect the chemical composition of the beverage, generating off flavors, undesirable aroma or altered appearance. It can also lead to broken packaging due to increasing pressure. Such spoilage can generate high economic losses and impact brand image.
For soft drinks, presence of spoilage yeast is also critical. Not only can presence of yeast impact the product similarly, it can lead to unwanted alcoholic fermentation.
Labor intensive and lengthy investigations after spoilage detection can generate further spoilage risks as origination can come from a wide variety of sources throughout the production process. Long investigations can increase losses as higher volume of spoiled product could be produced or production could be interrupted for a long period of time.Pall GeneDisc Technologies help industries to reduce these risks with solutions enabling the following:
- Implementation of rapid decision making tests for product release and key control points of the process
- Fast investigations after spoilage detection
GeneDisc System Benefits
Rapid — Pall's GeneDisc method allows a detection and identification of spoilage yeast in as fast as 30 h and direct monitoring, Brettanomyces quantification or colony indentification in 2 h.
Flexible — Analysis with enrichment ensures high sensitivity (as low as 1 cell/sample) when precise information is the priority. With direct monitoring, contamination assessment is sped up when quick results are required (reduced to 2 hours).
Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.
Modular — Screening and identification solutions allow testing on various sample types.
Solutions Designed for Beverage Industry
Accelerated decision-making – GeneDisc technology allows for early preventive controls to reduce risk of product spoilage and speed up batch release. For wine industry, quantification of Brettanomyces bruxellensis and spp. in 2 hours ensures real-time process control at critical steps (eg. between alcoholic and malolactic fermentations and during ageing).
Fast corrective actions implementation – Reduce negative financial impact of spoilage with rapid root cause analysis in case of contamination.
Tailor-made informative method – Two GeneDisc options for various testing strategies:
|GeneDisc Plate for Yeast Screening||GeneDisc Plate for Yeast ID|
|Screens for yeast||Detects and identifies 12 major spoilage yeast strains and species simultaneously|
|For testing yeast-free samples (e.g.bottled product, raw materials,soft drinks)||For assessing risk associated with spoilage detected using the GeneDisc Plate for Yeast Screening For testing wild spoilage yeast in samples containing process yeast
For Brettanomyces quantification in wine samples.
Reduced hands-on cost – Ease of use simplifies testing workflows and on-site implementation.
Spoilage Yeast ID
|Major Spoilage Yeast||
|Effect on Process||
How the System Works
|Enrichment Time||Down to 28 h - Optional|
|Sample Preparation Time||< 1 hour for 48 samples|
|Hands-on Time||About 45 minutes for 48 samples ( < 1 min/sample)|
|PCR Cycle Time||About 1 hour|
|Total Turnaround Time||
|GeneDisc Plate for Yeast ID – Targets||
|Limit of Detection (before enrichment)||Down to 1 cell/sample before enrichment.|
|Limit of Detection (direct monitoring)||Down to 1 cell/mL|
|Internal Positive Control||Ensure PCR reaction is not affected by the presence of inhibitors for each sample DNA extract|
- Poster: Performance Evaluation of the GeneDisc Method for Detection and Identification of Beer Spoilage Yeast, Jemmal S. et al., Pall Corporation.
- Poster: Performance Evaluation of the GeneDisc Method for Detection and Identification of Wine Spoilage Yeast, Jemmal S. et al., Pall Corporation.
- Application Bulletin : Reduce Storage Costs and Preserve Wine Quality with GeneDisc® Rapid Microbiology System
|GeneDisc Cycler Ultra-Lyser||—|
|GeneDisc DryBlock Heater 48||—|
|EGDCV3A||GeneDisc Cycler Base Unit|
|EGDSV3A||GeneDisc Cycler Sub Unit||—|
|PFOOD1100||Extraction Pack Food 1||100|
|GYEASCR106006||GeneDisc Plate for Yeast Screening||36|
|GYEASID103012||GeneDisc Plate for Yeast ID||36|
We also offer a full product range for pathogen detection in food and water as well as detection and identification of spoilage bacteria in beverages (Beer spoilage bacteria, TAB spoilage).
Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus) are also available.
For more information including part numbers please contact us.