Alicyclobacillus spp., also known as Thermophilic Acidophilic Bacteria (TAB), are spoilage organisms in the fruit juice industry. They can generate strong off flavors and off odors due to guaiacol or bromophenols production.
Their ability to grow at acidic pH and their resistance to normal thermal processing such as common pasteurization have made these bacteria a major quality issue for processors of juices and concentrates.
Contamination with these non-pathogenic bacteria can occur at various stages of the process. Rapid detection and identification of TAB spoilage is an informative solution to increase quality monitoring at identified critical points of the process to facilitate release of high quality products.
GeneDisc System Benefits
Rapid — While culture methods require up to 10 days to results, Pall's GeneDisc method allows a simultaneous detection and identification of Alicyclobacillus spp. in as fast as 2-3 hours.
Easy to use — GeneDisc method is designed for routine use and validated from sample to result. Implementing PCR (Polymerase Chain Reaction) has never been this easy.
Modular — System modularity fits your throughput needs: up to 48 samples can be analyzed in a one hour PCR run.
A Solution Designed for Beverage Industries
Save money – Shorter time to results reduces your storage cost by accelerating the release of your raw materials and /or final products. GeneDisc ease of use will also reduce your overall testing cost.
Get informative results – GeneDisc method allows the simultaneous detection of Alicyclobacillus spp. and identification of the 4 strains known to be main potential spoilers (A. acidoterrestris, A. acidophilus, A. cycloheptanicus, A. herbarius).
Avoid false results – Unique highly specific and sensitive tests enable a wide coverage of Alicyclobacillus species.
Alicyclobacillus spp. Identification
|Bacteria||Gram +, rod shapped, sporeforming bacillus|
|Distinctive features||Thermophilic (20 – 70 °C)
Acidophilic (pH 2.0 – 6.0)
|Identified in||Fruit juices and concentrates, ingredients such as sugar syrups and sugar powders, condensate water|
|Main spoilage associated strains||A. acidoterrestris
How the System Works
|Enrichment Time||Filterable samples |
|48 hours |
|Sample Preparation Time||Filterable samples |
|1 hour for 24 samples |
1 hour for 24 samples
|PCR Cycle Time||< 1 hour|
|Total Turnaround Time||Filterable samples |
|50 hours (for detection protocol) |
74 hours (for detection protocol)
2-3 hours (for relative quantification protocol)
|Hands On Time||Filterable samples |
|35 minutes for 24 samples |
25 minutes for 24 samples
|Limit Of Detection||Absence in 10 mL or 10 g of sample (for detection protocol) |
Down to 20 targeted bacteria/sample (for relative quantification protocol)
|Relative Quantification |
(for Dedicated Protocol)
|Down to 100 targeted bacteria/sample|
|Specificity||• Wide range of strains tested for inclusivity and exclusivity |
• Alicyclobacillus spp. assay covers over 200 strains
|Validated Matrices||• Fruit concentrate and fruit juice (including apple, orange, grapefruit, white and red grape, blueberry, mango) |
• Sugar powder and sugar syrup
• Condensate water
|Internal Positive Control||Detects presence of inhibitors in each sample DNA extract|
|EGDCV3A||GeneDisc Cycler Base Unit||-|
|EGDSV3A||GeneDisc Cycler Sub Unit||-|
|EGDUL1A230 (EU) |
|PFOOD1100||Extraction Pack Food 1||100|
|GTABSPO106006||GeneDisc Plate for TAB Spoilage||36|
We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage.
Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.
- Poster: Zoder P. et al. Novel Molecular Assay and Sample Preparation Method for the Detection of Alicyclobacillus in Fruit Juice Concentrates and Bottling Process Materials, Spoilers 2013.