For the rapid quantification of Legionella pneumophila and Legionella spp. in water
Legionellosis are infections caused by bacteria of the genus Legionella, generally by aerosol inhalation. With over 9,000 human cases reported per year, it is one of the most frequent causes of waterborne disease.Legionella species are capable of colonizing artificial water systems and equipment containing water, making this pathogen a major issue for:
- Industries where cooling towers can generate a wide dispersion of bacteria;
- Hospitals and care facilities where contaminated hot tap or shower waters are potential sources of Legionella outbreak and can effect weakened patients;
- Public-access buildings, such as spas and swimming pools, where aerosol dispersion is common;
- Fountains in public space.
Pall GeneDisc Technologies provides complete solutions to water system network managers for real time Legionella risk management.
GeneDisc System Benefits
Rapid — While culture methods require up to 12 days to obtain results, Pall's GeneDisc method allows a quantification of Legionella in as fast as 2 hours.
Easy to use — Matrix specific protocols are designed for routine use and validated from sample to result.
Modular — System modularity fits your throughput needs: up to 88 samples can be analyzed simultaneously.
A Solution Designed for Water Network Management
Reduce down-time cost – Shorter time to results enables reactive surveillance preventing delayed intervention on water networks and system closures.
Implement appropriate and effective disinfection measures – Mapping water system networks with the GeneDisc system identifies and targets contaminated pipework and water points.
In line with NF T 90-471 and ISO/TS 12869 – NF VALIDATION certified method against both standards.
Quantify Legionella spp. and Legionella pneumophila simultaneously – GeneDisc Plate Legionella DUO enables combined detection and quantification of both Legionella spp. and Legionella pneumophila using the same sample preparation and without any additional hands-on time.
Legionella pneumophila ID
|Bacteria||Gram – flagellated bacteria|
|Prevalence||Ubiquitous aquatic organism (from drinking water to cooling tower aerosols)|
|Disease||Legionellosis (Pontiac fever, Legionnaires' disease)|
|Human Cases Reported||6,296 (ECDC,2010)
3,346 (CDC, 2010)
|Related Clusters, Outbreaks||130 (ECDC, 2010)
10 for recreational water (CDC, 2007-2008)
12 for drinking water (CDC, 2007-2008)
How the System Works
|Total Turnaround Time||2 hours for clear water samples|
|3 hours for complex water samples|
|PCR Cycle Time||1 hour|
|Limit of Detection||5 GU/PCR well|
|Limit of Quantification||25 GU/PCR well|
|Quantification Range||25 – 250,000 GU/PCR well|
|PCR Efficiency (e)||75 % < e < 125 %|
|Optimal Recovery||> 50 %|
|Specificity||Wide range of strains tested for inclusivity and exclusivity|
|Legionella pneumophila Calibrator (external quantitative control)||Included in each GeneDisc Plate to validate accuracy of quantification|
|Internal Positive Control Per Sample Analysis||Detects presence of inhibitors in DNA extract sample|
- Yaradou, D. F. et al., 'Integrated real-time PCR for detection and monitoring of
Legionella pneumophila in water systems', Appl. Environ. Microbiol. 73: 1452-62.
- Dusserre, E. et al., 'A PCR-based method for monitoring Legionella pneumophila in
water samples detects viable but noncultivable Legionella that can recover their
cultivability', Appl. Environ. Microbiol. 74 (15): 4817-4824. 2008
- Alleron, L. et al., 'Long-term survival of Legionella pneumophila in the viable but
nonculturable state after monochloramine treatment', Curr Microbiol. 57(5): 497-502.
- Mietzner, S. M. et al. 'A rapid method for the detection of the nosocomial waterborne
pathogens Legionella spp. and Pseudomonas aeruginosa by real-time quantitative
PCR - A comparison with standard culture'. Presented at ICAAC/IDSA Meeting.
Washington, D.C. 2008
|EGDCV3A||GeneDisc Cycler Base Unit|
|EGDSV3A||GeneDisc Cycler Sub Unit||-|
(for clear and complex water samples)
(for complex water samples)
|ALEGPNE205||Legionella pneumophila Standard DNA
|PENVI1096||Extraction Pack Environment 01
(for clear or complex water samples)
||Extraction Pack Environment 03
(for clear water samples)
|GeneDisc Plate Legionella pneumophila||30
|GLEGSPP206006||GeneDisc Plate Legionella spp.||30|
|GLEGDUO206006||GeneDisc Plate Legionella DUO||30|
|GeneDisc Plate Legionella ID||36
We also offer a full product range for pathogen detection in food and for spoilage organisms in beverages.
Quantitative tests for pathogens in water (E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.