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GeneDisc® Technologies

For the rapid quantification of Legionella pneumophila and Legionella spp. in water

Legionellosis are infections caused by bacteria of the genus Legionella, generally by aerosol inhalation. With over 9,000 human cases reported per year, it is one of the most frequent causes of waterborne disease.

Legionella species are capable of colonizing artificial water systems and equipment containing water, making this pathogen a major issue for:
  • Industries where cooling towers can generate a wide dispersion of bacteria;
  • Hospitals and care facilities where contaminated hot tap or shower waters are potential sources of Legionella outbreak and can effect weakened patients;
  • Public-access buildings, such as spas and swimming pools, where aerosol dispersion is common;
  • Fountains in public space.

Pall GeneDisc Technologies provides complete solutions to water system network managers for real time Legionella risk management.

GeneDisc System Benefits

Rapid — While culture methods require up to 12 days to obtain results, Pall's GeneDisc method allows a quantification of Legionella in as fast as 2 hours.

Easy to use — Matrix specific protocols are designed for routine use and validated from sample to result.

Modular — System modularity fits your throughput needs: up to 88 samples can be analyzed simultaneously.

A Solution Designed for Water Network Management

Reduce down-time cost – Shorter time to results enables reactive surveillance preventing delayed intervention on water networks and system closures.

Implement appropriate and effective disinfection measures – Mapping water system networks with the GeneDisc system identifies and targets contaminated pipework and water points.

In line with NF T 90-471 and ISO/TS 12869 – NF VALIDATION certified method against both standards.

Quantify Legionella spp. and Legionella pneumophila simultaneously – GeneDisc Plate Legionella DUO enables combined detection and quantification of both Legionella spp. and Legionella pneumophila using the same sample preparation and without any additional hands-on time.

Legionella pneumophila ID

 
Bacteria Gram – flagellated bacteria
Prevalence Ubiquitous aquatic organism (from drinking water to cooling tower aerosols)
Disease Legionellosis (Pontiac fever, Legionnaires' disease)
Human Cases Reported 6,296 (ECDC,2010)
3,346 (CDC, 2010)
Related Clusters, Outbreaks 130 (ECDC, 2010)
10 for recreational water (CDC, 2007-2008)
12 for drinking water (CDC, 2007-2008)
 


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Description

For the rapid quantification of Legionella pneumophila and Legionella spp. in water

Legionellosis are infections caused by bacteria of the genus Legionella, generally by aerosol inhalation. With over 9,000 human cases reported per year, it is one of the most frequent causes of waterborne disease.

Legionella species are capable of colonizing artificial water systems and equipment containing water, making this pathogen a major issue for:
  • Industries where cooling towers can generate a wide dispersion of bacteria;
  • Hospitals and care facilities where contaminated hot tap or shower waters are potential sources of Legionella outbreak and can effect weakened patients;
  • Public-access buildings, such as spas and swimming pools, where aerosol dispersion is common;
  • Fountains in public space.

Pall GeneDisc Technologies provides complete solutions to water system network managers for real time Legionella risk management.

GeneDisc System Benefits

Rapid — While culture methods require up to 12 days to obtain results, Pall's GeneDisc method allows a quantification of Legionella in as fast as 2 hours.

Easy to use — Matrix specific protocols are designed for routine use and validated from sample to result.

Modular — System modularity fits your throughput needs: up to 88 samples can be analyzed simultaneously.

A Solution Designed for Water Network Management

Reduce down-time cost – Shorter time to results enables reactive surveillance preventing delayed intervention on water networks and system closures.

Implement appropriate and effective disinfection measures – Mapping water system networks with the GeneDisc system identifies and targets contaminated pipework and water points.

In line with NF T 90-471 and ISO/TS 12869 – NF VALIDATION certified method against both standards.

Quantify Legionella spp. and Legionella pneumophila simultaneously – GeneDisc Plate Legionella DUO enables combined detection and quantification of both Legionella spp. and Legionella pneumophila using the same sample preparation and without any additional hands-on time.

Legionella pneumophila ID

 
Bacteria Gram – flagellated bacteria
Prevalence Ubiquitous aquatic organism (from drinking water to cooling tower aerosols)
Disease Legionellosis (Pontiac fever, Legionnaires' disease)
Human Cases Reported 6,296 (ECDC,2010)
3,346 (CDC, 2010)
Related Clusters, Outbreaks 130 (ECDC, 2010)
10 for recreational water (CDC, 2007-2008)
12 for drinking water (CDC, 2007-2008)
 
Specifications

How the System Works


Technical Information

 
Total Turnaround Time 2 hours for clear water samples
3 hours for complex water samples
PCR Cycle Time 1 hour
Limit of Detection 5 GU/PCR well
Limit of Quantification 25 GU/PCR well
Quantification Range 25 – 250,000 GU/PCR well
PCR Efficiency (e) 75 % < e < 125 %
Optimal Recovery > 50 %
Specificity Wide range of strains tested for inclusivity and exclusivity
Legionella pneumophila Calibrator (external quantitative control) Included in each GeneDisc Plate to validate accuracy of quantification
Internal Positive Control Per Sample Analysis Detects presence of inhibitors in DNA extract sample
 
Type
Testing and Monitoring
Use
Diagnostics, Monitoring, Testing
Additional Information

Further Reading

  • Yaradou, D. F. et al., 'Integrated real-time PCR for detection and monitoring of
    Legionella pneumophila in water systems', Appl. Environ. Microbiol. 73: 1452-62.
    2007
  • Dusserre, E. et al., 'A PCR-based method for monitoring Legionella pneumophila in
    water samples detects viable but noncultivable Legionella that can recover their
    cultivability', Appl. Environ. Microbiol. 74 (15): 4817-4824. 2008
  • Alleron, L. et al., 'Long-term survival of Legionella pneumophila in the viable but
    nonculturable state after monochloramine treatment', Curr Microbiol. 57(5): 497-502.
    2008
  • Mietzner, S. M. et al. 'A rapid method for the detection of the nosocomial waterborne
    pathogens Legionella spp. and Pseudomonas aeruginosa by real-time quantitative
    PCR - A comparison with standard culture'. Presented at ICAAC/IDSA Meeting.
    Washington, D.C. 2008
Ordering Information
 
Part Number Description Samples/pack
Equipment
EGDCV3A GeneDisc Cycler Base Unit
EGDSV3A GeneDisc Cycler Sub Unit -
EGDUL1A230 (EU)
EGDUL1A120 (US)
GeneDisc Ultra-Lyser
(for clear and complex water samples)
-
EGDUP1A GeneDisc Ultra-Purifier
(for complex water samples)
-
 
Consumables
ALEGPNE205 Legionella pneumophila Standard DNA
(5 tubes)
-
PENVI1096 Extraction Pack Environment 01
(for clear or complex water samples)
96
PENVI3100

Extraction Pack Environment 03
(for clear water samples)
100
GLEGPNE206006
GLEGPNE212006
GeneDisc Plate Legionella pneumophila 30
66
GLEGSPP206006 GeneDisc Plate Legionella spp. 30
GLEGDUO206006 GeneDisc Plate Legionella DUO 30
GLEGOID206006
GLEGOID212006
GeneDisc Plate Legionella ID 36
72
 


We also offer a full product range for pathogen detection in food and for spoilage organisms in beverages.
Quantitative tests for pathogens in water (E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.

Application
Environmental Monitoring