GeneDisc Plate Listeria Identification product photo Primary L

GeneDisc Plate Listeria Identification

Listeria monocytogenes is an opportunistic foodborne pathogen that can affect a wide range of food products and can cause severe infections with high mortality rate to the elderly, immunodepressed persons and pregnant women. Since L. monocytogenes has the ability to grow slowly at refrigerated temperatures, it is a major concern for food companies. Some rare cases of listeriosis related to other Listeria spp. have also been reported.

According to MLG 8 and ISO 11290, detection of Listeria is based on a two steps procedure consisting of detection/ isolation followed by confirmation.

Conventional confirmation methods used for Listeria spp. identification are based on biochemical tests performed from isolated colonies. These methods results are typically obtained within 24 hours and might be ambiguous.

GeneDisc System Benefits

Rapid — Accelerate your control workflow. Result for colony confirmation is obtained in less than 1 hour.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

A Solution Designed For Food Industries

Easy to read – The GeneDisc Listeria ID method allows a clear discrimination amongst the 6 Listeria spp. from isolated colonies on agar plates.

Flexible – Designed for use with the main Listeria specific agars and chromogenic media.

Reliable – AOAC certified method


Listeria spp. Identification

 
Bacteria Gram + bacilli
Species List L. monocytogenes L. grayi
L. seeligeri
L. innocua
L. ivanovii
L. welshimeri
Major Human Pathogen L. monocytogenes
 

From Colony To Result In As Fast As One Hour




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Description

Listeria monocytogenes is an opportunistic foodborne pathogen that can affect a wide range of food products and can cause severe infections with high mortality rate to the elderly, immunodepressed persons and pregnant women. Since L. monocytogenes has the ability to grow slowly at refrigerated temperatures, it is a major concern for food companies. Some rare cases of listeriosis related to other Listeria spp. have also been reported.

According to MLG 8 and ISO 11290, detection of Listeria is based on a two steps procedure consisting of detection/ isolation followed by confirmation.

Conventional confirmation methods used for Listeria spp. identification are based on biochemical tests performed from isolated colonies. These methods results are typically obtained within 24 hours and might be ambiguous.

GeneDisc System Benefits

Rapid — Accelerate your control workflow. Result for colony confirmation is obtained in less than 1 hour.

Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.

A Solution Designed For Food Industries

Easy to read – The GeneDisc Listeria ID method allows a clear discrimination amongst the 6 Listeria spp. from isolated colonies on agar plates.

Flexible – Designed for use with the main Listeria specific agars and chromogenic media.

Reliable – AOAC certified method


Listeria spp. Identification

 
Bacteria Gram + bacilli
Species List L. monocytogenes L. grayi
L. seeligeri
L. innocua
L. ivanovii
L. welshimeri
Major Human Pathogen L. monocytogenes
 

From Colony To Result In As Fast As One Hour


Specifications
 
Sample Preparation Time < 15 minutes
PCR Cycle Time < 45 minutes
Total Turnaround Time < 1 hour
Specificity Wide range of strains tested for inclusivity and exclusivity
Internal Positive Control Per Sample Analysis Detects presence of inhibitors in DNA extract sample
Validation AOAC approved for colony confirmation from all major enrichment media
 
Type
Testing and Monitoring
Use
Diagnostics, Monitoring, Testing
Ordering Information
 
Part Number Description Samples/pack
Equipment
EGDUL1A230 (EU)
EGDUL1A120 (US)
GeneDisc Ultra-Lyser -
EGDCV3A GeneDisc Cycler Base Unit -
EGDSV3A GeneDisc Cycler Sub Unit -
Consumables
PFOOD1100 Extraction Pack Food 1 100
GLISTID106006 GeneDisc Listeria ID 36
 

We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage.

Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus…) are also available.

For more information including part numbers please contact us.

Further Readings

  • Poster: Desroche, N. et al., Evaluation of the performances of the GeneDisc ID Listeria real-time PCR assay for the rapid identification of Listeria species.
  • Poster: Crowley, E.S. et al. A Comparative Evaluation of the GeneDisc® Plate Listeria Identification Kit for the Identification and Discrimination of Listeria species. IAFP, Rhodes-Island, 2012.
Application
QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, QC Microbial Detection - Pathogens, Food Safety
Segment
Dairy
Food Contact Compliance & Safety
Food and Ingredients
Pet Food
Quality Control