Listeria monocytogenes is an opportunistic foodborne pathogen that can affect a wide range of food products and can cause severe infections with high mortality rate to the elderly, immunodepressed persons and pregnant women. Since L. monocytogenes has the ability to grow slowly at refrigerated temperatures, it is a major concern for food companies. Some rare cases of listeriosis related to other Listeria spp. have also been reported.
According to MLG 8 and ISO 11290, detection of Listeria is based on a two steps procedure consisting of detection/ isolation followed by confirmation.
Conventional confirmation methods used for Listeria spp. identification are based on biochemical tests performed from isolated colonies. These methods results are typically obtained within 24 hours and might be ambiguous.
GeneDisc System Benefits
Rapid — Accelerate your control workflow. Result for colony confirmation is obtained in less than 1 hour.
Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.
A Solution Designed For Food Industries
Easy to read – The GeneDisc Listeria ID method allows a clear discrimination amongst the 6 Listeria spp. from isolated colonies on agar plates.
Flexible – Designed for use with the main Listeria specific agars and chromogenic media.
Reliable – AOAC certified method
Listeria spp. Identification
|Bacteria||Gram + bacilli|
|Species List||L. monocytogenes L. grayi
|Major Human Pathogen||L. monocytogenes|
From Colony To Result In As Fast As One Hour
|Sample Preparation Time||< 15 minutes|
|PCR Cycle Time||< 45 minutes|
|Total Turnaround Time||< 1 hour|
|Specificity||Wide range of strains tested for inclusivity and exclusivity|
|Internal Positive Control Per Sample Analysis||Detects presence of inhibitors in DNA extract sample|
|Validation||AOAC approved for colony confirmation from all major enrichment media|
|EGDUL1A230 (EU) |
|EGDCV3A||GeneDisc Cycler Base Unit||-|
|EGDSV3A||GeneDisc Cycler Sub Unit||-|
|PFOOD1100||Extraction Pack Food 1||100|
|GLISTID106006||GeneDisc Listeria ID||36|
We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage.
Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus…) are also available.
For more information including part numbers please contact us.
- Poster: Desroche, N. et al., Evaluation of the performances of the GeneDisc ID Listeria real-time PCR assay for the rapid identification of Listeria species.
- Poster: Crowley, E.S. et al. A Comparative Evaluation of the GeneDisc® Plate Listeria Identification Kit for the Identification and Discrimination of Listeria species. IAFP, Rhodes-Island, 2012.