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GeneDisc® STEC 12, GSTEHEC112006
Product ID: GSTEHEC112006
Unit of Measure
1/PAC
1/PAC
Min Order Qty
1
1
Number of Samples per GeneDisc Plate
12
Number of Samples per Kit
72
GeneDisc® STEC/Salmonella 06, GSTECSL206006
Product ID: GSTECSL206006
Unit of Measure
1/PAC
1/PAC
Min Order Qty
1
1
Number of Samples per GeneDisc Plate
6
Number of Samples per Kit
36
GeneDisc® STEC/Salmonella 12, GSTECSL212006
Product ID: GSTECSL212006
Unit of Measure
1/PAC
1/PAC
Min Order Qty
1
1
Number of Samples per GeneDisc Plate
12
Number of Samples per Kit
72
GeneDisc® Plate STEC Top7, GTOP7EC106006
Product ID: GTOP7EC106006
Unit of Measure
1/PAC
1/PAC
Min Order Qty
1
1
Number of Samples per GeneDisc Plate
6
Number of Samples per Kit
36
Pack of 6 GeneDisc plate of 6 sectors
Product ID: GBPSPMI206006
Unit of Measure
1/EA
1/EA
Min Order Qty
1
1
Number of Samples per GeneDisc Plate
6
Number of Samples per Kit
36
Documents
Datasheets
Download:Description
A Flexible Approach for the Detection of Top 5 and Top 7 Shiga Toxic E. coli
Escherichia coli are common in human bacterial flora. Most of them are harmless, but some known as Shiga Toxic E. coli or STEC, can cause severe, sometimes lethal, pathologies.Main source of infection is contaminated food making it a major concern for food processors.
Among these STEC, several serogroups are more commonly related to human diseases. In Europe, STEC Top 5 serogroups include E. coliO157, O26, O103, O111 and O145. In the USA, serogroups O45 and O121 are added to this list making the STEC Top 7.
GeneDisc System Benefits
Rapid — Accelerate the batch release of your short shelf life products and raw materials. While other methods such as immunoassays or culture methods require up to 3 days to results, the Pall GeneDisc method allows a detection of pathogenic STEC in as fast as 10 hours.Easy to use — GeneDisc solutions are designed for routine use. Implementing PCR (Polymerase Chain Reaction) has never been this easy.
High throughput capability — Process up to 96 samples DNA extractions simultaneously in less than one hour.
Modular — System modularity fits your throughput needs: up to 96 samples can be analyzed in a one hour PCR run.
Specifications
Choose Your GeneDisc Solutions
Pall GeneDisc Technologies provide you with flexible solutions matching your needs for a reliable monitoring of STEC.Follow reference method | Reduce your rate of presumptive positive |
In line with MLG 5B and ISO/TS 13136 – With this method, a systematic screening based on virulence factors allows to discriminate pathogenic strains from non pathogenic ones. If result is positive, an identification of the Top 7 or Top 5 serogroups is performed. Test Salmonella spp. simultaneously – Analysis with Salmonella spp. is available and does not require any additional hands-on time nor enrichment. |
Enhanced workflow – This method enables to reduce the number of presumptive positive sample using a cutting edge approach. With this method, all targets – serogroups and virulence factors – are analyzed within one GeneDisc plate. High level of discrimination – An accurate virulence factor screening based on the association of these factors to serogroups provides a lower rate of presumptive positive than any other available method. |
Shiga Toxic E. coli Identification
Bacteria | Gram –, motile enterobacteria producing shigatoxins (stx) and intimin (eae) |
Food Vehicle | Large variety of food especially beef cattle and dairy products |
Disease | Gastro-enteritis, Hemorrhagic diarrhea, Hemolytic-uremic syndrome (HUS) |
Incidence of Cases (per 100,000 population) |
1.90 (FoodNet, 2010) |
Notification Rate (per 100,000 population) |
0.83 (EFSA, ECDC, 2010) |
Related Outbreaks | 2 (EFSA, ECDC, 2010) 25 (CDC, 2010) |
Related Recalls | 8 (FDA & FSIS, 2012) |
Related Alerts / Information | 22 (RASFF, 2011) |
Validations
Method | Part Number | Validation | Matrix | Time to Result (Down to) |
STEC | GSTEHEC1XX006 | AOAC-PTM | Raw Ground Beef/ Raw Beef Trim |
|
STEC & Salmonella spp. |
GSTECSL2XX006 | |||
STEC Top 7 | GTOP7EC106006 GSTECPL206006* |
|||
E. coli O157:H7 | GSTEHEC1XX006 GEHECID106006 |
NF VALIDATION |
Raw Beef Meat | 10 h |
Dairy products/ Vegetables products |
18 h | |||
E. coli O157:H7 & Salmonella spp. | GSTECSL2XX006 GEHECID106006 |
Raw Beef Meat | 10 h | |
Dairy products/ Vegetables products |
18 h | |||
All methods are approved by AOAC using both Extraction Pack Food 1 and Food 2
* Confirmation tool for the AOAC-PTM certified GeneDisc STEC Top 7 method
Technical Information
Enrichment Time | Down to 8 hours |
Sample Preparation Time | < 1 hour for 96 samples |
PCR Cycle Time | < 1 hour |
Total Turnaround Time | Down to 10 hours |
Limit Of Detection | 1 bacteria in 25 g of food sample 1 bacteria in 375 g of raw ground beef or raw beef trim |
Specificity | Wide range of strains tested for inclusivity and exclusivity |
Internal Positive Control Per Sample Analysis | Detects presence of inhibitors in each sample DNA extract |
Type
GeneDisc
Use
Monitoring, Testing
Ordering Information
Part Number | Description | Samples/pack |
Equipment | ||
EGDCV3A | GeneDisc Cycler Base Unit | - |
EGDSV3A | GeneDisc Cycler Sub Unit | - |
EGDUL1A230 (EU) EGDUL1A120 (US) |
GeneDisc Ultra-Lyser | - |
OR | ||
EGDBH96230 (EU) EGDBH96120 (US) |
GeneDisc DryBlock Heater 96 | - |
SPSKIT96 | Extraction Pack Food 2 Starter Kit | - |
Consumables | ||
PFOOD1100 | Extraction Pack Food 1 | 100 |
PFOOD2096 | Extraction Pack Food 2 (High throughput) | 96 |
GSTEHEC106006 GSTEHEC112006 |
GeneDisc ShigaToxic E. coli* | 36 72 |
GSTECSL206006 GSTECSL212006 |
GeneDisc ShigaToxic E. coli* & Salmonella spp. | 36 72 |
GEHECID106006 | GeneDisc EHEC 5 ID (H7, O26, O103, O111, O145) | 36 |
GTOP7EC106006 | GeneDisc STEC Top 7 (virulence genes and O157, O26, O103, O111, O145, O45, O121 serogroups) | 36 |
GSTECPL206006 | GeneDisc STEC Plus (virulence genes) | 36 |
*Includes identification of pathogenic E. coli O157
We also offer a full product range for pathogen detection in food and water and for spoilage organisms in beverage. Quantitative tests for pathogens in water (Legionella, E. coli, Enterococcus...) are also available. For more information including part numbers please contact us.
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