For Sample Preparation
Hydrophilic polyvinylidene fluoride (PVDF) membranes are commonly used in a variety of general filtration and sample preparation applications. Pall has multiple options for hydrophilic PVDF membrane available targeting specific applications ranging from sterile filtration to biological sample preparation to organic mobile phase filtration. All of the options available can be autoclaved and gamma sterilized as well as exhibit low biomolecule binding characteristics.
For sterile and general filtration applications, our unique, modified hydrophilic polyvinylidene fluoride (PVDF) membrane with ultra low binding properties, broad chemical and temperature resistance, and high flow rates is an excellent choice. The membrane is available in a 0.45 µm retention rating filtration of viscous fluids and prefiltration, 0.2 µm rated for sterilization, and 0.1 µm rated for sterilization and mycoplasma removal. We recommend using our hydrophilic PVDF in applications such as multi-plexed bead-based assays, the filtration of dilute protein samples, cold and hot Water For Injection (WFI), chemicals, aggressive solvents and sanitizing agents.“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
- General filtration
- Sample preparation
- Bead-based assays
- Mobile phase filtration
- Insert molding
- Heat (with support material)
Typical Membrane Characteristics
|Base Material||Grade||Pore Size (µm)||Thickness (mils)||Thickness (µm)|
|Unsupported polyvinylidene fluoride||Fluorodyne® II||0.1||2.8-3.5||71.1-88.9|
|Unsupported polyvinylidene fluoride||Fluorodyne II||0.2||3.5-4.4||88.9-111.8|
|Unsupported polyvinylidene fluoride||Fluorodyne II||0.45||2.4-4.3||61.0-109.2|
Typical Performance Characteristics
|Base Material||Grade||Pore Size (µm)||Water Bubble Point (psi)||Water Bubble Point (bar)||Water Flow (mL/min/cm2 @ 0.7 bar/10 psi)||Applications|
|Unsupported polyvinylidene fluoride||Fluorodyne II||0.1||29.0-36.0*||2.0-2.48||N/A||Sterile filtration|
|Unsupported polyvinylidene fluoride||Fluorodyne II||0.2||17.5-22.0*||1.21-1.52||N/A||Sterile filtration|
|Unsupported polyvinylidene fluoride||Fluorodyne II||0.45||11.0-15.5*||0.76-1.07||N/A||Sample prep, general filtration, bead-based assays|
*Values based on double-layer testing.
**HPLC qualification: Calculated ratio of maximum peak height at 254 nm to toluene standard is < 1.0.
Hydrophilic PVDF Membrane Exhibits Extremely Low Protein Binding
2.5 L of PBS containing 100 µg/mL BSA and approx 100k cpm/mL 125I-labeled BSA was perfused through 13 mm discs. After perfusion, discs are blotted on paper towels to remove excess liquid and are then placed in a gamma counter. µg/cm2 was calculated from cpm/discs. Very low binding was found on hydrophilic Fluorodyne PVDF membrane and on derivatized LoProdyne LP binding nylon membrane as compared to FluoroTrans, Fluorotrans W, and Biodyne A membranes.
The Biodyne, Fluorodyne, FluoroTrans, and LoProdyne names are trademarks of Pall Corporation and are not available for use.
Custom roll, sheet, and disc sizes available upon request. Please contact your local sales representative for additional information.
|SNY0720||Fluorodyne II membrane, 0.1 µm, 8" x 10" sheet||1/pkg|
|SNY0602||Fluorodyne II membrane, 0.2 µm, 8" x 10" sheet||1/pkg|
|BSP0861||Fluorodyne II membrane, 0.45µm, 3 m roll (Qty is 1/Pkg)||1/pkg|