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- Protein transfers
- Western blots
- Southern blots
- Northern blots
- Nucleic acid and protein dot blots
- BioTrace NT (nitrocellulose)
- 0.2 μm
- 101.6 - 190.5 μm (4.0 - 7.5 mils)
Less Burnthrough with Nitrocellulose Membranes
Prestained proteins were separated in a polyacrylamide gel and electrophoretically transferred to the indicated nitrocellulose membranes. A double layer of membrane was used, one directly against the gel, followed by the second layer. Signal intensity on the second layer is indicative of burnthrough, which can lead to loss of signal.
Protein Sample Prep & Detection
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