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Blue Trisacryl® M Affinity Chromatography Sorbent product photo Primary L

Blue Trisacryl® M Affinity Chromatography Sorbent

For the Purification of a Wide
Variety of Enzymes and Proteins

  • Strong bond of dye-to-sorbent prevents leakage of the dye in normal working conditions
  • Excellent chemical stability is attributed to the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism
  • Excellent mechanical stability allows high flow rates with working pressures up to 3 bar (300 kPa, 45 psi)
  • Basic matrix is Trisacryl GF2000, a macroporous non-ionic sorbent. Cibacron Blue F3GA dye is strongly bound to the matrix through a six-carbon spacer arm

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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Blue Trisacryl M Affinity Chromatography Resin

Blue Trisacryl M (100 mL)
List Price
$ 610.00
Blue Trisacryl M (1000 mL)
List Price
$ 5,530.00

Sorbent - Blue Trisacryl M

List Price
$ 200.00
Description

For the Purification of a Wide
Variety of Enzymes and Proteins

  • Strong bond of dye-to-sorbent prevents leakage of the dye in normal working conditions
  • Excellent chemical stability is attributed to the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism
  • Excellent mechanical stability allows high flow rates with working pressures up to 3 bar (300 kPa, 45 psi)
  • Basic matrix is Trisacryl GF2000, a macroporous non-ionic sorbent. Cibacron Blue F3GA dye is strongly bound to the matrix through a six-carbon spacer arm

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Albumin removal increases the resolution of both 1D and 2D electrophoresis
  • Designed for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors
  • The interaction mechanism between Cibacron Blue F3GA and proteins involves one or more of the following: stereospecific recognition of NAD analogs; electrostatic and hydrophobic interaction; and/or electron exchange
Specifications
 
Particle size 40 – 80 μm
Ligand Cibacron blue 3GA
Exclusion limit 107 Da
Capacity for human albumin1 ≥10 mg/mL
Thermal stability 2 to 121 °C
Working pH 4 to 10
Cleaning pH 1 to 12 (short term)
Working pressure at 100 cm/hr Up to 3 bar (45 psi)
 

1 Determined in PBS buffer using 5 mg/mL protein, column dimensions 16 mm I.D. x 6 cm bed height, flow rate 25 cm/hr
Performance

Analytical Separation of Human Plasma Proteins on Blue Trisacryl M Affinity Chromatography Sorbent  


Analytical Separation of Human Plasma Proteins on Blue Trisacryl M Affinity Chromatography Sorbent

Column: 1.6 cm I.D. x 10 cm; Buffer: 0.05 M Tris-HCI, pH 8.8; Elution performed by a continuous sodium chloride gradient from 0 to 3 M; Flow rate: 100 cm/h; Separation time: 180 min; Temperature: 20 °C.
Type
Chromatography Sorbent
Use
Affinity Chromatography
Application
Chromatography