Jumbosep™ Centrifugal Devices product photo Primary L

Jumbosep™ Centrifugal Devices

Convenient and Reliable Concentration, Purification, and Diafiltration of 20 to 60 mL Biological Samples

  • Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes
  • Provides high recoveries, typically > 90%
  • Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding
  • Versatile Omega membrane is available in a variety of MWCOs, color-coded for easy identification
  • Built-in deadstop prevents spinning to dryness
  • Unique sealing mechanism prevents retentate leakage and filtrate contamination
  • Economical - Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused


* Required

Jumbosep Centrifugal Device Membrane Inserts

List Price
$ 242.65
List Price
$ 242.65
List Price
$ 242.65
List Price
$ 244.44
List Price
$ 246.88

Jumbosep Centrifugal Device Starter Kits

List Price
$ 180.20
List Price
$ 240.32
List Price
$ 240.32
List Price
$ 240.32
List Price
$ 235.61
List Price
$ 240.32

Jumbosep Spare Parts and Accessories

Jumbosep Spare Parts and Accessories - Filtrate receiver and cap (12/pkg)
List Price
$ 159.10
Jumbosep Spare Parts and Accessories - Sample reservoir and cap (12/pkg)
List Price
$ 214.86

Spare Parts and Accessories

List Price
$ 140.04
Description

Convenient and Reliable Concentration, Purification, and Diafiltration of 20 to 60 mL Biological Samples

  • Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes
  • Provides high recoveries, typically > 90%
  • Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding
  • Versatile Omega membrane is available in a variety of MWCOs, color-coded for easy identification
  • Built-in deadstop prevents spinning to dryness
  • Unique sealing mechanism prevents retentate leakage and filtrate contamination
  • Economical - Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused
Applications
Replaces dialysis, chemical precipitation, and lyophilization in the following applications:
  • Concentrating and desalting proteins
  • Exchanging buffer or removing salt from chromatography eluates and gradient fractions
  • Separating biomolecules from cell culture supernatants
  • Concentrating or removing viruses
  • Performing crude fractionation of protein mixtures
  • Removing debris and particulates from cell lysates
Specifications

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) membrane
  • Sample Reservoir and Filtrate Receiver: Polysulfone
  • Sample Reservoir Cap: Polyethylene
  • Insert Without Membrane: High density polyethylene
  • Filtrate Receiver Cap and Insert Release: Polypropylene

Effective Filtration Area

  • 15.2 cm2

Dimensions

  • Outside Diameter (Maximum): 6 cm (2.4 in.)
  • Overall Height (Fully Assembled with Cap): 11.3 cm (4.5 in.)

Capacities

  • Maximum Sample Volume: 60 mL
  • Final Concentrate Volume: 3.5 - 4 mL
  • Maximum Filtrate Receiver Volume: 60 mL
  • Hold-up Volume (membrane/support): 0.2 mL

Operating Temperature Range

  • 0 - 40 °C (32 - 104 °F)

pH Range

  • 1 - 14

Centrifuge

  • Swinging bucket rotor is required that accepts flat-bottomed 250 mL bottles and is capable of spinning at up to 3,000 x g

Sanitization

  • Provided non-sterile. The entire device, including the filter media, may be sanitized by filtering 70% ethanol through it prior to use. The sample reservoir and the filtrate receiver can be autoclaved. Do not autoclave the filter media.
Performance
Jumbosep™ Device Reduces Processing Time Over Other Devices

Jumbosep Centrifugal Device Reduces Processing Time

1 mg/mL BSA solution was processed in each of the above devices until a 15-fold concentration is achieved.
 

Concentration Time

Jumbosep Centrifugal Device Concentration Time

Concentrate dilute protein samples in less than 30 minutes with 10, 30, and 100K Jumbosep devices.
Type
Centrifugal device
Use
Ultrafiltration
Application
Plasmid Purification
Product Summary
Replaces dialysis, chemical precipitation, and lyophilization. Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes. Provides high recoveries, typically > 90%.
Segment
DNA & RNA Purification
Protein Sample Prep & Detection
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