Macrosep® Advance Centrifugal Devices

Quickly Concentrates Up To
20 mL of Biological Sample

  • Rapidly concentrates 20 mL sample volumes to 0.5 mL
  • Provides high recoveries, typically > 90%
  • Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
  • Versatile Omega membrane is available in a variety of MWCOs
  • Built-in deadstop prevents spinning to dryness
  • Color-coded for easy identification
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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Macrosep Advance Centrifugal Devices With Supor Membrane

List Price
$ 231.37
List Price
$ 907.82
List Price
$ 231.37
List Price
$ 907.82

Macrosep Advance Centrifugal Devices with Omega Membrane

List Price
$ 62.01
List Price
$ 233.70
List Price
$ 951.45
List Price
$ 61.39
List Price
$ 231.37
List Price
$ 907.82
List Price
$ 61.39
List Price
$ 231.37
List Price
$ 907.82
List Price
$ 61.39
List Price
$ 231.37
List Price
$ 907.82
List Price
$ 61.39
List Price
$ 231.37
List Price
$ 907.82
Description

Quickly Concentrates Up To
20 mL of Biological Sample

  • Rapidly concentrates 20 mL sample volumes to 0.5 mL
  • Provides high recoveries, typically > 90%
  • Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
  • Versatile Omega membrane is available in a variety of MWCOs
  • Built-in deadstop prevents spinning to dryness
  • Color-coded for easy identification
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications

Ultrafiltration

  • Concentrate and desalt proteins
  • Exchange buffer or remove salt of chromatography eluates and gradient fractions
  • Recover proteins or other molecules from cell culture supernatants

Microfiltration

  • Remove particulate from aqueous solutions and clinical samples
Specifications

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
  • Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
  • Paddle: Polyethylene

Effective Filtration Area

  • 7.2 cm2

Dimensions

  • Diameter: 29 mm (1.2 in.)
  • Length: 12.0 cm (4.7 in.)

Operating Temperature Range

  • 0 - 40 °C (32 - 104 °F)

Capacities

  • Maximum Sample Volume: 20 mL
  • Final Concentrate Volume:
       450 μL (swinging bucket)
       1.2 - 1.5 mL (45º angle rotor)
       1.5 mL (34º angle rotor)
  • Filtrate Receiver Volume: 22 mL
  • Hold-Up Volume: 80 μL (membrane and paddle)

pH Range

  • 1 - 14

Maximum Centrifugal Force

  • 5,000 x g (ultrafiltration)
  • 14,000 x g (microfiltration)

Centrifuge

  • Fits centrifuges that accept standard 50 mL conical end tubes

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.
Performance

Macrosep Advance Centrifugal Devices: Reduced Spin Time



Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.

Macrosep Advance Centrifugal Devices: Retention Efficiency


Macrosep Advance Centrifugal Devices: Retention Efficiency
Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.
Type
Centrifugal device
Use
Ultrafiltration
Application
Plasmid Purification
Product Summary
Macrosep® Advance centifugal filters quickly concentrate up to 20 mL of biological samples. Rapidly concentrates 20 mL sample volumes to 0.5 mL. Provides high recoveries, typically > 90%.
Segment
DNA & RNA Purification
Protein Sample Prep & Detection
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