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FluoroTrans® PVDF Transfer Membranes product photo Primary L

FluoroTrans® PVDF Transfer Membranes

Sensitive Protein Detection with Low Background and Very Low Burnthrough

  • Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
  • High sensitivity for small peptides.
  • High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.
  • FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing. The medium demonstrates good signal-to-noise ratios with standard detection systems, and immobilized proteins can be used directly for sequencing, or visually detected with common staining reagents including Amido Black, Ponceau S, and colloidal gold. 
  • FluoroTrans W membrane is optimized for Western transfer applications. This membrane allows for sensitive protein detection with low background and very low protein burnthrough. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."


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FluoroTrans PVDF Transfer Membrane

FluoroTrans W PVDF Transfer Membrane

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Datasheets

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Description

Sensitive Protein Detection with Low Background and Very Low Burnthrough

  • Naturally hydrophobic polyvinylidene fluoride is ideal for a wide variety of protein-analysis applications. The family of FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions.
  • High sensitivity for small peptides.
  • High protein binding capacity. Typically absorbs 50% more protein than nylon or nitrocellulose.
  • FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing. The medium demonstrates good signal-to-noise ratios with standard detection systems, and immobilized proteins can be used directly for sequencing, or visually detected with common staining reagents including Amido Black, Ponceau S, and colloidal gold. 
  • FluoroTrans W membrane is optimized for Western transfer applications. This membrane allows for sensitive protein detection with low background and very low protein burnthrough. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."

Specifications

Filter Media

  • FluoroTrans PVDF (hydrophobic polyvinylidene fluoride)

Pore Size

  • 0.2 μm

Typical Thickness

  • 127 μm (5.0 mils)

Applications

FluoroTrans W Membrane

  • Southern transfers

FluoroTrans PVDF Membrane

  • N-terminal protein sequencing
  • Fluorescent western transfers
  • FluoroTrans media have high tensile strength and will not tear, crack, or curl during handling. This allows for easy removal of target bands for protein sequencing applications.

Performance

FluoroTrans Membrane Has Excellent Sensitivity, Signal,
and Background in Western Transfers


FluoroTrans Membrane Has Excellent Sensitivity, Signal, and Background in Western Transfers

Rabbit reticulocyte lysate (Amersham) was loaded in lanes of polyacrylamide gels at f.s., 1/3 and 1/10 dilutions. After electrophoresis, proteins were transferred to membranes. Membranes were stained with 0.1% Amido Black, 45% methanol, and 2% acetic acid for 4 minutes; then destained for 5 minutes with two changes of 90% methanol and 2% acetic acid. Stained membranes were rinsed in water and air dried.

Type

Membrane Filters

Use

Binding

Product Summary

Ideal for a wide variety of protein-analysis applications, these membranes with 0.2µm pore sizes feature naturally hydrophobic polyvinylidene fluoride filter media.

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