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AcroPrep™ Advance Filter Plates for Lysate Clearance
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AcroPrep Advance Filter Plates for Lysate Clearance
Spare Parts and Accessories
AcroPrep Advance multi-well plate lids (10/pkg)
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Description
Prefilter Provides Efficient Clarification
Prefilter Provides Efficient Clarification
of Highly Particulated Samples
- Integrated prefilter yields consistent filtration of samples with high levels of gross particulate
- New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume
- Innovative outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross-contamination
- Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules
Applications
- Removal of bacterial debris prior to plasmid purification
- Removal of bacterial and cellular debris prior to protein purification
- Clearance of gross particulates
Specifications
Materials of Construction
- Filter Media: 3 μm glass fiber/0.2 μm Supor® membrane and 3 μm glass fiber/1.2 μm Supor membrane
- Plate Housing: Polyproylene
- Lid: Polystyrene
Dimensions
- Length: 12.8 cm (5.0 in.)
- Width: 8.6 cm (3.4 in.)
- Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
- Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.); 2 mL: 4.7 cm (1.9 in.)
Well-Bottom Area
- 0.25 cm2
Recommended Working Volume
- 350 μL: < 300 μL
- 1 mL: < 900 μL
- 2 mL: < 1.9 mL
Recommended Operating Vacuum
- > 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
- 1,500 x g
Typical Processing Time
- PN 8040
- Vacuum: 2 seconds
- Centrifuge: < 2 minutes
- PN 8075, 8175, 8275
- Vacuum: 9 seconds
- Centrifuge: < 2 minutes
Typical Hold-Up Volume
- PN 8040 - Vacuum: 17 μL
- PN 8075, 8175, 8275 - Vacuum: 13 μL
Performance
Glass Fiber and Supor Membrane Allow Complete Recovery of Plasmid DNA from E. coli Lysates
Purified pCAT plasmid DNA was spiked in TE buffer at 25 μg/mL, 50 μg/mL, and 100 μg/mL and filtered through Pall and Competitor A’s prefilter plates. Percent recovery was calculated by comparing OD260 post-filtration to a standard curve of unfiltered samples.
Type
Filter Plates
Application
Protein Fractionation
Product Summary
AcroPrep™ Advance Filter Plates for Lysate Clearance are manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules.
