AcroPrep Advance 96-well Long Tip Filter Plate for Nucleic Acid Binding
Spare Parts and Accessories
AcroPrep Advance 96 well, 1 mL Filter Plate with Nucleic Acid binding media, long tips, natural housing, 5/pk
The Pall AcroPrep Advance 96-well Long Tip Filter Plate for Nucleic Acid Binding (Pall NAB plate) incorporates a silica-based quartz glass fiber media to allow for efficient binding of DNA and RNA, while providing smooth flow and rapid processing of samples. This media offers researchers the flexibility to purify plasmid DNA from bacteria, and genomic DNA or total RNA from cell culture samples: a single plate for multiple applications. Reducing the chance of cross contamination is critical for reproducible quality results. Pall's new long tip plate minimizes hanging drop formation thus reducing the possibility of cross contamination. The Pall NAB plate is a multipurpose plate providing flexibility in applications, reduced risk of cross contamination and smooth flow for sample processing.
Maximum yields and quality of nucleic acid purification
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New outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross contamination
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Silica-based quartz glass fiber media that allows efficient binding of DNA and RNA, while providing smooth flow and rapid processing of samples
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Manufactured in accordance with specifications for the Society of Biomolecular Screening (ANSI/SBS x-2004) for multi-well plates allowing entire DNA purification process to be performed on automated equipment
Plasmid DNA purification
- Transfections
- Cloning
- Sanger sequencing
Genomic Purification
- PCR
- Real-time PCR
- Next Generation Sequencing (NGS)
Total RNA Purification
- Real-time quantitative PCR
- Microarrays
- cDNA library construction
- Northern blot analysis
Materials of Construction
- Filter Media: Silica based quartz glass fiber
- Housing: Polypropylene
Effective Filtration Area
- 0.25 cm2
Sample Volume Range
- 50 uL – 900 uL
Recommended Operating Vacuum
- >25.4 cm Hg (10 in. Hg)
Recommended Positive Pressure
- >5 PSI (start at 5 PSI and increase in 2 PSI increments until flow is achieved in a timely manner)*
AcroPrep Advance 96-well Long Tip Filter plate with redesigned outlet tips reduce well-to-well crosstalk by minimizing the formation of hanging drops
Figure 1
Pall NAB plate with redesigned outlet tips reduce well-to-well crosstalk by minimizing the formation of hanging drops.
Hanging-drop (A) and crosstalk (B) volumes for the Pall NAB plate and Pall's previous generation plasmid DNA purification plate (Pall ST: PN 8132). For hanging-drop volume, the total hanging drop volume was determined for three plates, and the average volume/well calculated. For crosstalk volume, the data represents the average of 216 data points.
Genomic DNA Isolation from freshly harvested HEK293 and CHO cells compared to Competitive commercial product
Figure 2
Genomic DNA Isolation from freshly harvested HEK293 and CHO cells compared to competitive commercial product.
Efficiency and reproducibility of genomic DNA isolation from freshly harvested HEK293 and CHO cells (top and bottom panels, respectively) are very similar for the Pall NAB plate (blue bars) and the commercial plate (red bars). Bars indicate an average of 8 samples. Error bars indicate standard deviation.
Protocols.
Click to download
- Protocol - Genomic DNA Purification From Plant Tissue Using Pall AcroprepTM Advance 96-well Long Tip Filter Plate For Nucleic Acid Binding And Commercially Available Buffers
- Protocol - Standard Reagent Protocol for Genomic DNA Isolation From Plant Tissue Using Pall Acroprep™ Advanced 96-well Long Tip Filter Plate for Nucleic Acid Binding
