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HEA and PPA HyperCel™ Mixed-mode Chromatography Sorbents product photo Primary L

HEA and PPA HyperCel™ Mixed-mode Chromatography Sorbents

For Selectivities Not Accessible with
Traditional Ion Exchange or HIC

  • Chromatographic behavior “mixed-mode” based on a combination of electrostatic and hydrophobic properties of the protein and ligands
  • Provides direct hydrophobic capture of proteins at low ionic strength
  • Can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation that cannot be performed by methods like ion exchange
  • Can be used in a “physiological-like” environment (no need for pH adjustment of the feedstream; no, or limited, addition of salt)
  • Orthogonal to ion exchange or other chromatography steps

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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HEA HyperCel Mixed-mode Chromatography Resin

HEA HyperCel (1000 mL)
List Price
$ 3,680.00

PPA HyperCel Mixed-mode Chromatography Resin

PPA HyperCel (1000 mL)
List Price
$ 3,680.00

Sorbent - HEA HyperCel

List Price
$ 520.00
List Price
$ 143.00

Sorbent - PPA HyperCel

List Price
$ 520.00
List Price
$ 140.00
Description

For Selectivities Not Accessible with
Traditional Ion Exchange or HIC

  • Chromatographic behavior “mixed-mode” based on a combination of electrostatic and hydrophobic properties of the protein and ligands
  • Provides direct hydrophobic capture of proteins at low ionic strength
  • Can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation that cannot be performed by methods like ion exchange
  • Can be used in a “physiological-like” environment (no need for pH adjustment of the feedstream; no, or limited, addition of salt)
  • Orthogonal to ion exchange or other chromatography steps

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Fractionation of a protein mix
  • Separation of partially-purified polyclonal IgG from major plasma impurities
  • Process polishing steps
Specifications

Average Particle Size

  • 90 µm

Bead Composition

  • High porosity cross-linked cellulose

Dynamic Binding Capacity for BSA (10% breakthrough)1

  • 40 - 60 mg/mL

Ligand

  • Aliphatic (HEA): Hexylamine
  • Aromatic (PPA): Phenylpropylamine

Adsorption pH

  • 7 - 9

Elution pH

  • By gradient or step elution, e.g. 7.0 - 2.6

Working pH

  • 2 - 12

Pressure Resistance

  • < 3 bar (300 kPa, 44 psi)

Storage Temperature

  • 2 - 8 °C (36 - 46 °F)

1Determined using 5 mg/mL BSA in PBS; flow rate: 100 cm/h
Performance

Comparison Between HEA and PPA HyperCel, MEP HyperCel, Conventional HIC, and Anion Exchange Sorbents 

Comparison Between HEA and PPA HyperCel, MEP HyperCel, Conventional HIC, and Anion Exchange Sorbents

Adsorption/desorption of Bovine Serum Albumin (BSA), in PBS buffer, pH 7.4. Data shows that BSA is efficiently retained on both HEA and PPA HyperCel sorbents, but is poorly retained on MEP HyperCel sorbent (as this ligand is antibody-selective). In PBS, without lyotropic salt addition, low binding of BSA to both Phenyl and Hexyl HIC sorbents is observed. The anion exchange (DEAE) sorbent also did not bind BSA at these nonoptimal conditions (pH 7.4 and too high salt concentration).
Type
Chromatography Sorbent
Use
Mixed-mode Chromatography
Application
Chromatography