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HA Ultrogel® Hydroxyapatite Chromatography Sorbent product photo Primary L

HA Ultrogel® Hydroxyapatite Chromatography Sorbent

For the Separation of Proteins,
Peptides, and Nucleic Acids

  • High binding capacity and high flow rates
  • Structure provides excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature
  • Easy cleaning with sodium hydroxide
  • Scalable from research and development to manufacturing

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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HA Ultrogel® Hydroxyapatite Chromatography Sorbent, Laboratory Scale Volumes

HA Ultrogel Hydroxyapatite (1000 mL)
List Price
$ 2,270.00
HA Ultrogel Hydroxyapatite (500 mL)
List Price
$ 1,180.00

Sorbent - HA Ultrogel Hydroxyapatite

List Price
$ 275.00
List Price
$ 135.00
Description

For the Separation of Proteins,
Peptides, and Nucleic Acids

  • High binding capacity and high flow rates
  • Structure provides excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature
  • Easy cleaning with sodium hydroxide
  • Scalable from research and development to manufacturing

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Separation of basic proteins (Cytochrome C, Lysozyme, etc.)
  • Separtaion of human serum proteins and plant proteins such as lectins, glycoproteins, transferases, kinases, and trehalases
  • Separation of phosphate dependent proteins and exzymes as well as
    DNA-dependent enzymes
  • Can be used to remove DNA and endotoxins
Specifications

Average Particle Size

  • 120 µm

Hydroxyapatite Content (Weight/Volume)

  • 40%

Agarose (Weight/Volume)

  • 4%

Exclusion Limit

  • > 5,000,000 Da

Capacity for Cytochrome C1

  • > 7 mg/mL

Capacity for BSA2

  • < 7 mg/mL

Working pH

  • 5 - 13 (Note: Do not use < pH 4.0.)

Storage Temperature

  • 2 - 30 ºC (36 - 86 °F)
  • 2 - 8 ºC (36 - 46 °F) after opening

1Determined at 50% breakthrough using 5 mg/mL Cytochrome C diluted 50/50 in 10 mM PBS, pH 6.8
at 30 cm/h.
2Determined at 50% breakthrough using 1 mg/mL BSA diluted 50/50 in 10 mM PBS, pH 6.8 at 12.5 cm/h.
Performance

Separation of a Mixture of Ribonuclease and Phytohemagglutinins (PHA-EL)


HA Ultrogel: Separation of a Mixture of Ribonuclease and Phytohemagglutinins (PHA-EL)

Column: 1.6 x 6.5 cm; Sample: 1 mg of protein mixture composed of ribonuclease (MW 14,700) and PHA-ELs (Erythroagglutinating and lymphostimulating Phytohemagglutinin) (MW 128,000) from Phaseolus vulgaris, in 1 mL of 5 mM potassium phophate, pH 6.8; Elution gradient: 5 mM to 500 mM potassium phosphate, pH 6.8; Flow rate: 14.4 cm/h.

Separation of Trypsin and Chymotrypsin from a Porcine Pancreatic Enzyme Extract


HA Ultrogel: Separation of Trypsin and Chymotrypsin from a Porcine Pancreatic Enzyme Extract

Column: 1.6 x 5 cm; Sample: 30 mg protein in 1 mL of 5 mM phosphate buffer, pH 6.8; Gradient: 5 to 200 mM sodium phosphate, ph 6.8; Flow rate: 10cm/h; Temperature: 10 °C; Histogram with broken line: trypsiin activity; Histogram with solid line: chymotrypsin activity. Spec. Ac.: specific activity in U/mg. tr: trypsin, ch: chymostrypsin. Trypsin activity was primarily found in the peak eluted by 50 mM phosphate where the chymotrypsin was eluted by 100 mM phosphate. The final yilad was approximately 50%.
Type
Chromatography Sorbent
Use
Chromatography
Application
Chromatography