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Heparin HyperD™ M Affinity Chromatography Sorbent product photo Primary L

Heparin HyperD™ M Affinity Chromatography Sorbent

For the Purification of Biological
Molecules that Bind to Heparin

  • Design combines the desirable characteristics of a soft, high speed, high capacity hydrogel with the high dimensional stability of a rigid bead
  • Leakage is minimized due to the stable chemical link of the heparin molecule to the sorbent
  • Rapid packing due to the high density of the bead
  • Can be packed in a variety of column sizes

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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Heparin HyperD™ M Affinity Chromatography Resin

Heparin HyperD M (100 mL)
List Price
$ 690.00
Heparin HyperD M (1000 mL)
List Price
$ 6,282.00

Sorbent

List Price
$ 245.00
Description

For the Purification of Biological
Molecules that Bind to Heparin

  • Design combines the desirable characteristics of a soft, high speed, high capacity hydrogel with the high dimensional stability of a rigid bead
  • Leakage is minimized due to the stable chemical link of the heparin molecule to the sorbent
  • Rapid packing due to the high density of the bead
  • Can be packed in a variety of column sizes

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
For the purification of: 
  • Enzymes (lipoprotein lipase, coagulation enzymes, superoxide dismutase)
  • Growth factors (fibroblast growth factor, Schwan cell growth factor)
  • Extracellular matrix proteins (fibronectin, vitronectin)
  • Nucleic acid binding protein, hormone receptors, and lipoproteins
Specifications

Average Particle Size

  • 80 µm

Dynamic Binding Capacity for Human ATIII (10% Breakthrough)1

  • > 25 mg/mL

Ligand

  • Porcine Heparin

Immobilized Heparin/mL Sorbent

  • 5 - 10 mg/mL

Working pH

  • 3 - 13

Pressure Resistance

  • 70 bar (7,000 kPa, 1,000 psi)

Storage Temperature

  • 2 - 8 ° C (36 - 46 ° F)

1Determined using human ATIII at 72.5 UI/mL in 20 mM TrisHCl, 0.3 M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2 M NaCl, pH 7.4 at a flow rate of 600 cm/h, 10 cm (4 in.) bed height.
Performance

Dynamic Binding Capacity vs. Linear Velocity


Heparin HyperD M Chromatography Sorbent Dynamic Binding Capacity vs. Linear Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Sample: HU ATIII at 72.5 μL/mL; Equilibration buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.

Pressure vs. Linear Flow Velocity


Heparin HyperD M Chromatography Sorbent Pressure vs. Linear Flow Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.
Type
Chromatography Sorbent
Use
Affinity Chromatography
Application
Chromatography