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PRC Pre-Packed Chromatography Columns product photo Primary L

PRC Pre-Packed Chromatography Columns

For Ion Exchange and Mixed-Mode Chromatography Laboratory Applications

  • Convenient – Ready-to-use 1 and 5 mL pre-packed columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems, such as ÄKTA systems.
  • Consistent – Screen Pall ion exchange and mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
  • Versatile – Two or more columns can be connected in series to increase the column height and more closely model real pilot scale conditions or scale-down applications.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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HyperCel STAR AX PRC Prepacked Columns

List Price
$ 95.00
List Price
$ 190.00

PRC Pre-Packed Chromatography Columns with Ion Exchange Sorbents

List Price
$ 95.00
List Price
$ 95.00

PRC Pre-Packed Chromatography Columns with Mixed-Mode Sorbents

List Price
$ 95.00
List Price
$ 95.00
List Price
$ 95.00
List Price
$ 190.00
List Price
$ 190.00
List Price
$ 190.00
Description

For Ion Exchange and Mixed-Mode Chromatography Laboratory Applications

  • Convenient – Ready-to-use 1 and 5 mL pre-packed columns.
  • Easy to use – Direct connection to commonly-used laboratory chromatography systems, such as ÄKTA systems.
  • Consistent – Screen Pall ion exchange and mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.
  • Versatile – Two or more columns can be connected in series to increase the column height and more closely model real pilot scale conditions or scale-down applications.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications

Ion Exchange Chromatography


HyperCel STAR AX sorbent is an industry-scalable anion exchange chromatography sorbent designed for high productivity protein capture at moderate to physiological or higher salt conductivity (2 to 15 mS/cm), typical of undiluted biological feedstocks (i.e., mammalian cell culture supernatants, E. coli feedstock, plasma, others…).
  • High dynamic binding capacity (DBC) at short residence time (2 minutes or lower)
  • Direct capture of protein from undiluted feedstock at moderate or high conductivity
  • Excellent flow rate properties for fast feedstock processing
  • Distinctive selectivity, consistent in a broad conductivity range (2-15 mS/cm)
  • Enhanced process economics
Q and CM Ceramic HyperD™ sorbents have a high capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. Typical features include:
  • Good dynamic binding capacity independent of flow rate.
  • Direct capture with high binding capacity for IgG at conductivities of 10-15 mS/cm on CM Ceramic HyperD sorbent.
Ceramic HyperD and HyperCel sorbents have different selectivities. The PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

Applications

  • Native and recombinant proteins
  • Plasmids
  • Vaccines
  • Monoclonal and polyclonal antibodies
  • Plasma derivatives
  • Biopharmaceuticals

Mixed-Mode Chromatography

MEP, HEA, and PPA HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants. They provide a unique separation mechanism different from conventional methods. All ligands operate predominantly by hydrophobic interaction. This "HIC-like" interaction typically takes place without the addition of lyotropic salt.

Applications

  • MEP HyperCel sorbent
    • Antibody capture
    • Alternative to conventional hydrophobic interaction
  • MEP, HEA, and PPA HyperCel sorbents
    • No salt/low salt alternative to hydrophobic interaction
Specifications

Materials of Construction

Body and End Caps: Molded polypropylene
17 μm Frit: PP/PE (polypropylene/polyethylene)

Outer Dimensions

1 mL: 10 x 100 mm
5 mL: 11.5 x 140 mm

Connections

Built-in 10-32 fittings
For HPLC/MPLC/ÄKTA systems, direct connection with 1/16 in. tubing and 10-32 fittings. For connecting to systems with M6 or 1/4-28 fittings, consult the appropriate system manual for necessary fittings and adapters.

Maximum Operating Pressure

1 mL: 20 barg (290 psig)
5 mL: 30 barg (435 psig)

Volume

1 mL: 5 mm ID x 50 mm
5 mL: 8 mm ID x 100 mm

Storage Solution

HyperCel STAR AX Sorbent: 30% isopropanol/100 mM sodium phosphate, pH 4.3
Ceramic HyperD F Sorbent: 20% ethanol/150 mM NaCl

Working Pressure

HyperCel STAR AX Sorbent: < 0.5 barg (7 psig)
Ceramic HyperD F Sorbent: < 1.5 barg (22 psig)
Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

 
Ion Exchange Chemistry Average Particle Size (μm) Ionizable Groups (μEq/mL) Dynamic Capacity (mg/mL)1
Q Ceramic HyperD F Quaternary
amine
50 ≥ 250 ≥ 852
CM Ceramic HyperD F Carboxymethyl 50 ≥ 250 ≥ 603
HyperCel STAR AX 80 µm >1006
 

 
Mixed-Mode Ligand Average Particle Size (μm) Ligand Density (μmole/mL) Dynamic Capacity (mg/mL)1
MEP HyperCel 4-mercaptoethyl- pyridine (pKa = 4.8) 90 80-125 ≥ 204
HEA HyperCel Hexylamine (aliphatic) (pKa = 8.0) 90 58-84 ≥ 405
PPA HyperCel Phenylpropyla mine (aromatic) (pKa = 8.0) 90 58-80 ≥ 405
 
1 Determined at 10% breakthrough using:
2 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h.
3 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h.
4 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
5 5 mg/mL BSA in PBS, flow rate 100 cm/h.
6 5 mg/mL BSA in 25 mM Tris-HCl, 0.14 M NaCl at 2 min residence time.
Performance

Figure 1

Separation of Cytochrome C, BSA, and Human Transferrin on a PRC Q Ceramic HyperD F Pre-Packed Column
Figure 1
Column: Q Ceramic HyperD F PRC pre-packed column (5 mm ID x 50 mm). Volume: 1 mL linear flow rate: 150 cm/h. Equilibration and wash: 50 mM Tris-HCl, pH 8.6. Load: 100 μL (5 mg/mL cytochrome C, 20 mg/mL BSA, and 20 mg/mL human transferrin in equilibration buffer). Elution: 50 mM


Figure 2

Separation of Lysozyme, α-Chymotrypsinogen, Ovalbumin, and BSA on a PRC PPA HyperCel Pre-Packed Column
Figure 3
Column: PPA HyperCel PRC pre-packed column (5 mm ID x 50 mm). Volume: 1 mL linear flow rate: 75 cm/h. Equilibration and wash: PBS, pH 7.4. Load: 500 μL (lysozyme, α-chymotrypsinogen, ovalbumin, and BSA, all at 2 mg/mL in equilibration buffer). Elution: by pH step gradient in phosphate/citrate buffer, pH 7.0, 5.4, and 2.6.
Type
Chromatography Consumables
Use
Chromatography
Application
Chromatography