Free ground shipping on Laboratory products for registered Credit Card users.
SDR HyperD™ Detergent Removal Chromatography Sorbent product photo Primary L

SDR HyperD™ Detergent Removal Chromatography Sorbent

Unique Sorbent Designed to Eliminate Detergent from Biological Fluids

  • High dynamic binding capacity for many different detergents.
  • High recovery for proteins.
  • High adsorption capacity for small, hydrophobic molecules.
  • Stable in acid, polar organic, and oxidizing solutions.
  • Composed of silica beads in which the pore volume is filled with a three-dimensional crosslinked hydrophobic polymer. The small pore size, particle size distribution (40 to 100 μm), and hydrophobic nature of the chemical groups make SDR HyperD sorbent ideal for detergent removal.

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


* Required
Need Help?

SDR HyperD Detergent Removal Chromatography Sorbent

SDR HyperD (1000 mL)
List Price
$ 1,565.00

Sorbent

SDR HyperD (100 mL)
List Price
$ 360.00
SDR HyperD (25 mL)
List Price
$ 120.00
Description

Unique Sorbent Designed to Eliminate Detergent from Biological Fluids

  • High dynamic binding capacity for many different detergents.
  • High recovery for proteins.
  • High adsorption capacity for small, hydrophobic molecules.
  • Stable in acid, polar organic, and oxidizing solutions.
  • Composed of silica beads in which the pore volume is filled with a three-dimensional crosslinked hydrophobic polymer. The small pore size, particle size distribution (40 to 100 μm), and hydrophobic nature of the chemical groups make SDR HyperD sorbent ideal for detergent removal.

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Binds detergent molecules typically used in viral inactivation processes [i.e., Tri-n-Butyl Phosphate (TnBP) and Tritons X-100]
  • Removal of both ionic and zwitter ionic detergents from biological solutions
Specifications

Average Particle Size

  • 70 μm

Nature of Polymer

  • Hydrophobic, long aliphatic chains bind solvents

Recommended Residence Time

  • 5 - 15 min

Binding Capacity for Triton X-100

  • 60 - 80 mg/mL1

Working pH

  • 2 - 12

Pressure Resistance

  • 70 bar (7,000 kPa, 1,000 psi)

Storage Temperature

  • 2 - 30 ºC (36 - 86 ºF)
  • 2 - 8 ºC (36 - 46 ºF) after opening
  • Do not freeze

1Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h.
Performance

SDR HyperD Detergent Removal: Spin Column Format (< 0.2 mL Volume)

 
Detergent Removal Efficiency from a Protein Sample1
Volume (Wt./v) + 1% + 5% + 10%
ASB-14 > 99% > 99% 80-90%
ASB-14 + 6M urea > 99% 80-90% NR
CHAPS > 99% > 99% > 99%
CHAPS + 6M urea > 99% > 99% 80-90%
SDS > 99% > 99% > 99%
SDS + 0.1 M NaCl > 99% > 99% > 99%
 
110.2 mL of 5 mg/mL BSA in the presence of detergents (Wt./v). Bio-Rad dye binding assay used to follow removal of detergents. NR denotes “Not Recommended” as this detergent loading would exceed the column capacity.

Using a rapid spin column format, the binding capacity of SDR for detergents is still very high. In all cases, > 99% of detergent removed from 0.2 mL of a 1% solution.
Type
Chromatography Consumables
Use
Chromatography
Application
Chromatography