Free ground shipping
on Laboratory products for registered Credit Card users.

Acrodisc® Units with Mustang® Q and S Membranes
* Required
Need Help?
Complementary Products
Acrodisc unit with Mustang Q membrane, 0.8 µm, 25 mm, non-sterile (10/pkg (blister packs))
Mustang E Membrane in Acrodisc Capsules
Description
Disposable Chromatography Units with High Binding Capacities and Fast Flow Rates
- Fast flow rates. Recommended flow rates of 1 to 4 mL/minute do not affect recovery rates.
- Mustang Q membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles.
- Mustang S membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles.
- Disposable 25 mm units are designed for single laboratory use only.
- Direct scale up to capsules with Mustang Q and S membranes for larger-volume applications.
Applications
Mustang Q Membrane
- Provides contaminant removal such as DNA viral particle, host cell proteins, or endotoxin
- Ideal for isolation via capture and release of plasmid DNA, virus, or target protein from a complex mixture
- Offers protein polishing for negativelycharged proteins
- Purifies virus and oligonucleotides
Mustang S Membrane
- Purifies and concentrates positively-charged proteins and viral particles
- Ideal for evaluation, process development, and validation
Specifications
Materials of Construction
- Filter Media: Mustang Q and Mustang S membranes
- Housing: Polypropylene
Pore Size
- 0.8 μm
Membrane Bed Volume
- 0.18 mL
Inlet/Outlet Connections
- Female luer lock inlet, male slip luer outlet
Maximum Operating Pressure
- 5.5 bar (550 kPa, 80 psi) at ambient temperature 21 - 24 ºC (70 - 75 ºF)
Biological Safety
- Passes United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>. Mustang Q membrane testing performed at 72 ºC (161 ºF) and Mustang S membrane at 50 ºC (122 ºF) after preconditioning.
Typical Operating Temperature
- 21 - 24 ºC (70 - 75 ºF)
Recommended Flow Rate
- 1 - 4 mL/min; flow rate will vary with type of solutions, concentration of proteins, and other components.
Typical Mean Dynamic Binding Capacity1
- Mustang Q Membrane
- DNA: 3.6 mg/Acrodisc unit or 30 mg/mL membrane volume (mv)
- BSA: 10 mg/Acrodisc unit or 70 mg/mL membrane volume (mv)
- Mustang S Membrane
- Lysozyme: 8 mg per Acrodisc unit or 47 mg/mL membrane volume (mv)
- Human IgG: 11 mg per Acrodisc unit or 60 mg/mL membrane volume (mv)
1Note on optimization: The yield is contingent on type of DNA, size and copy number of plasmid, concentration of protein, ionic strength, and pH of buffer.
Performance
Acrodisc Unit with Mustang Q Membrane:

Acrodisc Unit with Mustang Q Membrane:

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1 minutes and was calculated as 54 mg/mL using:
Acrodisc Unit with Mustang S Membrane:

Acrodisc Unit with Mustang S Membrane:
A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
Acrodisc Unit with Mustang Q Membrane:
Resolution with BSA and Goat lgG

The conditions used to generate data for the resolution graph above include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in 25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column volume (CV); flow rate: 2.3 mL/min (13 cv/min); sample loading: 4% of total binding capacity.
Acrodisc Unit with Mustang Q Membrane:
Dynamic Binding with BSA

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1 minutes and was calculated as 54 mg/mL using:
flow rate (2.3 mL/min) X initial protein BSA concentration (0.524 mg/mL) X time (8.1 min)
membrane bed volume of Mustang Q membrane in 25 mm Acrodisc unit (0.18 mL)
Acrodisc Unit with Mustang S Membrane:
Resolution with Cytochrome C and Lysozyme
The conditions used to generate data for the graph above include buffer: 10mM MES pH 5.5; salt: 1M NaCl in 10mM MES pH 5.5; gradient: 0 to 1M NaCl in 50 CV; flow rate: 2.3 mL/min (13 cv/min).
Acrodisc Unit with Mustang S Membrane:
Dynamic Binding with Lysozyme
A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
flow rate (2.3 mL/min) X initial protein Lysozyme concentration (0.512 mg/mL) X time (8.0 min)
membrane bed volume of Mustang S membrane in 25 mm Acrodisc unit (0.18 mL)
Application
Chromatography