Mustang Q Membrane

• Provides contaminant removal such as DNA viral particle, host cell proteins, or endotoxin
• Ideal for isolation via capture and release of plasmid DNA, virus, or target protein from a complex mixture
• Offers protein polishing for negativelycharged proteins
• Purifies virus and oligonucleotides

Mustang S Membrane

• Purifies and concentrates positively-charged proteins and viral particles
• Ideal for evaluation, process development, and validation

Materials of Construction

• Filter Media: Mustang Q and Mustang S membranes
• Housing: Polypropylene

Pore Size

• 0.8 μm

Membrane Bed Volume

• 0.18 mL

Inlet/Outlet Connections

• Female luer lock inlet, male slip luer outlet

Maximum Operating Pressure

• 5.5 bar (550 kPa, 80 psi) at ambient temperature 21 - 24 ºC (70 - 75 ºF)

Biological Safety

• Passes United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>. Mustang Q membrane testing performed at 72 ºC (161 ºF) and Mustang S membrane at 50 ºC (122 ºF) after preconditioning.

Typical Operating Temperature

• 21 - 24 ºC (70 - 75 ºF)

Recommended Flow Rate

• 1 - 4 mL/min; flow rate will vary with type of solutions, concentration of proteins, and other components.

Typical Mean Dynamic Binding Capacity¹

• Mustang Q Membrane
  • DNA: 3.6 mg/Acrodisc unit or 30 mg/mL membrane volume (mv)
  • BSA: 10 mg/Acrodisc unit or 70 mg/mL membrane volume (mv)
• Mustang S Membrane
  • Lysozyme: 8 mg per Acrodisc unit or 47 mg/mL membrane volume (mv)
  • Human IgG: 11 mg per Acrodisc unit or 60 mg/mL membrane volume (mv)

¹Note on optimization: The yield is contingent on type of DNA, size and copy number of plasmid, concentration of protein, ionic strength, and pH of buffer.

Acrodisc Unit with Mustang Q Membrane:
Resolution with BSA and Goat lgG

The conditions used to generate data for the resolution graph above include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in 25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column volume (CV); flow rate: 2.3 mL/min (13 cv/min); sample loading: 4% of total binding capacity.

Acrodisc Unit with Mustang Q Membrane:
Dynamic Binding with BSA

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1 minutes and was calculated as 54 mg/mL using:
flow rate (2.3 mL/min) X initial protein BSA concentration (0.524 mg/mL) X time (8.1 min)
membrane bed volume of Mustang Q membrane in 25 mm Acrodisc unit (0.18 mL)

Acrodisc Unit with Mustang S Membrane:
Resolution with Cytochrome C and Lysozyme

The conditions used to generate data for the graph above include buffer: 10mM MES pH 5.5; salt: 1M NaCl in 10mM MES pH 5.5; gradient: 0 to 1M NaCl in 50 CV; flow rate: 2.3 mL/min (13 cv/min).

Acrodisc Unit with Mustang S Membrane:
Dynamic Binding with Lysozyme

A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
flow rate (2.3 mL/min) X initial protein Lysozyme concentration (0.512 mg/mL) X time (8.0 min)
membrane bed volume of Mustang S membrane in 25 mm Acrodisc unit (0.18 mL)

• AcroPrep™ Advance 96-Well Filter Plates for Protein Purification