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Unique Sorbent Designed to Eliminate Detergent from Biological Fluids
- High dynamic binding capacity for many different detergents.
- High recovery for proteins.
- High adsorption capacity for small, hydrophobic molecules.
- Stable in acid, polar organic, and oxidizing solutions.
- Composed of silica beads in which the pore volume is filled with a three-dimensional crosslinked hydrophobic polymer. The small pore size, particle size distribution (40 to 100 μm), and hydrophobic nature of the chemical groups make SDR HyperD sorbent ideal for detergent removal.
- Binds detergent molecules typically used in viral inactivation processes [i.e., Tri-n-Butyl Phosphate (TnBP) and Tritons X-100]
- Removal of both ionic and zwitter ionic detergents from biological solutions
Average Particle Size
- 70 μm
Nature of Polymer
- Hydrophobic, long aliphatic chains bind solvents
Recommended Residence Time
- 5 - 15 min
Binding Capacity for Triton X-100
- 60 - 80 mg/mL1
- 2 - 12
- 70 bar (7,000 kPa, 1,000 psi)
- 2 - 30 ºC (36 - 86 ºF)
- 2 - 8 ºC (36 - 46 ºF) after opening
- Do not freeze
1Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h.
SDR HyperD Detergent Removal: Spin Column Format (< 0.2 mL Volume)
|Detergent||Removal Efficiency from a Protein Sample1|
|Volume (Wt./v)||+ 1%||+ 5%||+ 10%|
|ASB-14||> 99%||> 99%||80-90%|
|ASB-14 + 6M urea||> 99%||80-90%||NR|
|CHAPS||> 99%||> 99%||> 99%|
|CHAPS + 6M urea||> 99%||> 99%||80-90%|
|SDS||> 99%||> 99%||> 99%|
|SDS + 0.1 M NaCl||> 99%||> 99%||> 99%|
110.2 mL of 5 mg/mL BSA in the presence of detergents (Wt./v). Bio-Rad dye binding assay used to follow removal of detergents. NR denotes “Not Recommended” as this detergent loading would exceed the column capacity.
Using a rapid spin column format, the binding capacity of SDR for detergents is still very high. In all cases, > 99% of detergent removed from 0.2 mL of a 1% solution.