Minimate™ Tangential Flow Filtration Systems product photo Primary L

Minimate™ Tangential Flow Filtration Systems

Minimate Streamlines Laboratory-Scale Concentration,
Desalting, and Buffer Exchange Processes

  • Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing. The Minimate TFF system includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly.
  • High concentration factors. The low system working volume achieved through the use of a conical bottom reservoir and compact design enables high concentration factors from up to 1 L or more of sample to be achieved. Concentrate your sample down to as little as 5 mL.
  • All wetted components are made from low protein binding, chemically resistant, biologically safe materials.
  • Minimal sample carryover. The system has a very low volume and interior fluid contact area. Fluid does not pass through and is not retained in the pump mechanism.
  • Roller head peristaltic pumps provide gentle processing and are the choice for critical applications such as fragile biomolecules.
  • System components are designed for easy assembly and disassembly; no tools required. Tubing and fitting replacement is simple and quick. Slip nuts for luer locks eliminate tubing kinks.


* Required
List Price
$ 3,776.31
List Price
$ 2,097.92
List Price
$ 3,776.31
List Price
$ 2,097.92
List Price
$ 3,739.64
List Price
$ 2,138.66
Description

Minimate Streamlines Laboratory-Scale Concentration,
Desalting, and Buffer Exchange Processes

  • Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing. The Minimate TFF system includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly.
  • High concentration factors. The low system working volume achieved through the use of a conical bottom reservoir and compact design enables high concentration factors from up to 1 L or more of sample to be achieved. Concentrate your sample down to as little as 5 mL.
  • All wetted components are made from low protein binding, chemically resistant, biologically safe materials.
  • Minimal sample carryover. The system has a very low volume and interior fluid contact area. Fluid does not pass through and is not retained in the pump mechanism.
  • Roller head peristaltic pumps provide gentle processing and are the choice for critical applications such as fragile biomolecules.
  • System components are designed for easy assembly and disassembly; no tools required. Tubing and fitting replacement is simple and quick. Slip nuts for luer locks eliminate tubing kinks.
Applications
  • Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides)
  • Recover antibodies or recombinant proteins from clarified cell culture media
  • Process metal sensitive enzymes and molecules
  • Separate (fractionate) large from small biomolecules
  • Concentrate viruses or gene therapy vectors
  • Prepare samples prior to column chromatography
  • Concentrate samples after gel filtration 
  • Depyrogenate water, buffers, and media solutions
Specifications

Materials of Construction

  • Reservoir: Polysulfone
  • Reservoir Cover: Polypropylene
  • Reservoir O-Ring: Buna-N rubber (nitrile)
  • Magnetic Stir Bar: PTFE coated
  • Gauge Wetted Parts: Type 316L stainless steel
  • SS Fitting O-ring: EPDM rubber
  • Gauge Mounting Block: Polypropylene
  • Luer Fittings: Polypropylene and stainless steel
  • Tubing: PharMed #16
  • Three-Way Valves: Polycarbonate body, acetal core
  • Drip Tray: Urethane

 
  Minimate TFF System Minimate TFF Reservoir Assembly Only1
Includes Peristaltic pump, pump head pressure gauge, valves, reservoir with stir bar, built-in stir plate on a drip tray Pressure gauge, valves, reservoir with stir bar, built-in stir plate on a drip tray
Dimensions 30.7 cm W x 48.2 cm D x 20.8 cm H (12.1 in. W x 19 in. D x 8.2 in. H) 30.7 cm W x 20.3 cm D x 20.8 cm H (12.1 in. W x 8.0 in. D x 8.2 in. H)
Weight 6.0 kg (13.2 lbs.) 1.0 kg (2.2 lbs.)
Maximum Inlet Pressure 4.1 bar (410 kPa, 60 psi) 4.1 bar (410 kPa, 60 psi)
Operating Temperature Range 0 - 50 °C (0 - 106 °F) 0 - 50 °C (0 - 106 °F)
Recirculation Flow Rate 10 - 240 mL/min 10 - 240 mL/min
Minimum System Working Volume < 5 mL < 5 mL
 

1User will need to supply a MasterFlex L/S variable-speed pump with Easy-Load pump head or equivalent.

Trademarks used are the property of Pittway Corporation:Buna, and Cole-Parmer: Easyoad, L/S, Masterflex
Performance
Designed to work with the Minimate™ TFF Capsule, this easy-to-use system can process sample volumes up to 1 L or more and efficiently concentrate samples to as little as 5 mL. Subsequent desalting or buffer exchange steps can be run on the same system with minimal user intervention.

The ready-to-use Minimate TFF System includes a Masterflex L/S variable speed peristaltic pump, Masterflex L/S Easy-Load pump head, as well as a 0 - 4 bar (0 - 60 psi) pressure gauge, valves, 500 mL reservoir with magnetic stir bar, and stir plate all assembled on a compact drip tray. The use of a peristaltic pump with disposable tubing and fittings minimizes sample cross contamination while providing gentle processing for biomolecules. All wetted parts meet United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>.

The reservoir is designed to hold up to 500 mL of sample and provides efficient mixing of product. The tapered bottom and optimally located feed and return ports reduce the system hold-up volume, which allows high concentration factors to be achieved. The reservoir lid seals tightly allowing additional sample volume or diafiltration solution to be drawn directly into the reservoir without the need of a transfer pump. Vacuum is created as filtrate is generated through the TFF device allowing continuous diafiltration to be performed. When it's time to retrieve your sample, simply open the valve on the reservoir outlet and pump out your sample.

Trademarks are the property of Cole-Parmer
Type
TFF System
Use
Ultrafiltration
Application
Protein Concentration & Buffer Exchange
Product Summary
These disposable tangential flow filtration devices accelerate and simplify scale-up bioprocessing applications. Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing.
Segment
Protein Sample Prep & Detection
Shop Laboratory Products
Tangential Flow Filtration