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Minimate™ Tangential Flow Filtration Systems

Minimate™ Streamlines Laboratory-Scale Concentration,
Desalting, and Buffer Exchange Processes

  • Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing. The Minimate TFF system includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly.
  • High concentration factors. The low system working volume achieved through the use of a conical bottom reservoir and compact design enables high concentration factors from up to 1 L or more of sample to be achieved. Concentrate your sample down to as little as 5 mL.
  • All wetted components are made from low protein binding, chemically resistant, biologically safe materials.
  • Minimal sample carryover. The system has a very low volume and interior fluid contact area. Fluid does not pass through and is not retained in the pump mechanism.
  • Roller head peristaltic pumps provide gentle processing and are the choice for critical applications such as fragile biomolecules.
  • System components are designed for easy assembly and disassembly; no tools required. Tubing and fitting replacement is simple and quick. Slip nuts for luer locks eliminate tubing kinks.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."
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Minimate™ EVO TFF System
Minimate EVO Reservoir Kit
Minimate EVO Drip Tray Kit
Minimate EVO Stir Plate Kit
Minimate EVO Pump Drive
Minimate EVO Pump Head
Minimate EVO Fittings Kit

Description

Minimate™ Streamlines Laboratory-Scale Concentration,
Desalting, and Buffer Exchange Processes

  • Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing. The Minimate TFF system includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly.
  • High concentration factors. The low system working volume achieved through the use of a conical bottom reservoir and compact design enables high concentration factors from up to 1 L or more of sample to be achieved. Concentrate your sample down to as little as 5 mL.
  • All wetted components are made from low protein binding, chemically resistant, biologically safe materials.
  • Minimal sample carryover. The system has a very low volume and interior fluid contact area. Fluid does not pass through and is not retained in the pump mechanism.
  • Roller head peristaltic pumps provide gentle processing and are the choice for critical applications such as fragile biomolecules.
  • System components are designed for easy assembly and disassembly; no tools required. Tubing and fitting replacement is simple and quick. Slip nuts for luer locks eliminate tubing kinks.
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."

Specifications

Materials of Construction

  • Reservoir: Polysulfone
  • Reservoir Cover: Polypropylene
  • Reservoir O-Ring: Buna♦-N rubber (nitrile)
  • Magnetic Stir Bar: PTFE coated
  • Gauge Wetted Parts: Type 316L stainless steel
  • SS Fitting O-ring: EPDM rubber
  • Gauge Mounting Block: Polypropylene
  • Luer Fittings: Polypropylene and stainless steel
  • Tubing: PharMed♦ #16
  • Three-Way Valves: Polycarbonate body, acetal core
  • Drip Tray: Urethane

 
  Minimate EVO TFF System
Includes Peristaltic pump, pump head pressure gauge, valves, reservoir with stir bar, built-in stir plate on a drip tray
Dimensions 30.7 cm W x 48.2 cm D x 20.8 cm H (12.1 in. W x 19 in. D x 8.2 in. H)
Weight 6.0 kg (13.2 lbs.)
Maximum Inlet Pressure 4.1 bar (410 kPa, 60 psi)
Operating Temperature Range 0 - 50 °C (0 - 106 °F)
Recirculation Flow Rate 10 - 240 mL/min
Minimum System Working Volume < 15 mL
 

♦Trademarks used are the property of Pittway Corporation:Buna, and Cole-Parmer: Easyoad, L/S, Masterflex

Applications

  • Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides)
  • Recover antibodies or recombinant proteins from clarified cell culture media
  • Process metal sensitive enzymes and molecules
  • Separate (fractionate) large from small biomolecules
  • Concentrate viruses or gene therapy vectors
  • Prepare samples prior to column chromatography
  • Concentrate samples after gel filtration 
  • Depyrogenate water, buffers, and media solutions

Performance

Designed to work with the Minimate™ TFF Capsule, this easy-to-use system can process sample volumes up to 1 L or more and efficiently concentrate samples to as little as 5 mL. Subsequent desalting or buffer exchange steps can be run on the same system with minimal user intervention.

The ready-to-use Minimate TFF System includes a Masterflex♦ L/S♦ variable speed peristaltic pump, Masterflex L/S Easy-Load♦ pump head, as well as a 0 - 4 bar (0 - 60 psi) pressure gauge, valves, 500 mL reservoir with magnetic stir bar, and stir plate all assembled on a compact drip tray. The use of a peristaltic pump with disposable tubing and fittings minimizes sample cross contamination while providing gentle processing for biomolecules. All wetted parts meet United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>.

The reservoir is designed to hold up to 500 mL of sample and provides efficient mixing of product. The tapered bottom and optimally located feed and return ports reduce the system hold-up volume, which allows high concentration factors to be achieved. The reservoir lid seals tightly allowing additional sample volume or diafiltration solution to be drawn directly into the reservoir without the need of a transfer pump. Vacuum is created as filtrate is generated through the TFF device allowing continuous diafiltration to be performed. When it's time to retrieve your sample, simply open the valve on the reservoir outlet and pump out your sample.

♦Trademarks are the property of Cole-Parmer

Type

Systems

FAQs

1 Q. How many times can a Minimate™ capsules be reused?
A. There are no specific guidelines for the total number of times a Minimate capsule can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Minimate capsules prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of capsules should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Minimate capsule drops below 50% of the original NWP value, it is suggested that a new capsule is used.

2 Q. How many times can a Centramate™ cassettes be reused?
A. There are no specific guidelines for the total number of times a Centramate cassette can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Centramate cassettes prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of cassettes should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Centramate cassette drops below 50% of the original NWP value, it is suggested that a new cassette is used.

3 Q. When should I order Centramate™ vs Centramate PE?
A. Certain applications require a fluid path that is free from metal. Our standard Centramate has stainless steel fittings and a 100% stainless steel cassette holder, so it would not be a good option for these applications. Pall’s Centramate PE has plastic fittings and the fluid path of the holder is polyethylene plastic. The pressure gauge that comes standard with the Centramate PE will have a stainless steel pathway, but the ¼” NPT fittings the system uses makes it easy to use a pressure gauge whose wettable parts will be free from metal.

4 Q. What do I need to attach an LV Centramate™ to the Minimate™ EVO system?
A. Pall recommends ordering the Minimate EVO Fittings kit, PN 97014, to get the additional 3-way stop cocks, hose barb adapters, and tubing needed to properly connect the LV Centramate holder to the Minimate EVO system. Contact your Pall representative or technical support if you have further questions.

5 Q. How many cassettes can I stack in a Centramate™ or Centramate PE?
A. You can stack up to 5 0.1 m2 T12 cassettes on Centramate, giving you up to 0.5m2 of effective filtration area. For the Centramate PE, only 4 0.1 m2 T12 cassettes will fit because of the thicker PE base. Stacking multiple cassettes in the holders increases the surface area of the membrane which gives a higher permeate flow rate and increases the throughput.

6 Q. Guidance on average recirculation flow rates and permeate flux rates for the Minimate™, LV Centramate™, Centramate PE/SS.
A. For the Minimate we recommend a recirculation flow rate of 30-40 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 4 ml/min. For the LV Centramate we recommend a recirculation flow rate of 120-160 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 15 ml/min. For the Centramate and Centramate PE we recommend a recirculation flow rate of 600-800 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 75 ml/min. Note: These are typical values. Actual values may be higher or lower depending on the membr

Product Summary

These disposable tangential flow filtration devices accelerate and simplify scale-up bioprocessing applications. Plug-n-play design. Plug in a Minimate TFF capsule, add sample, and turn on the pump to start processing.

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