Nanosep® and Nanosep MF Centrifugal Devices

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

  • Ensures rapid processing of samples 
  • Typical recoveries are > 90%
  • Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
  • A wide range of MWCOs, color-coded for easy identification
  • Constructed of low-binding polypropylene 
  • Ultrasonically welded seals prevent bypass or seal failure
  • Fits standard centrifuge rotors that accept 1.5 mL tubes


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Nanosep MF Centrifugal Devices with Bio-Inert® Membrane

0.2 µm, aqua (24/pkg)
List Price
$ 68.90
0.2 µm, aqua (100/pkg)
List Price
$ 243.28
0.2 µm, aqua (500/pkg)
List Price
$ 1,043.25
0.45 µm, wildberry (24/pkg)
List Price
$ 68.90
0.45 µm, wildberry (100/pkg)
List Price
$ 243.28
0.45 µm, wildberry (500/pkg)
List Price
$ 1,043.25

Nanosep MF Centrifugal Devices with GHP Membrane

0.45 µm, clear (100/pkg)
List Price
$ 335.80
0.45 µm, clear (500/pkg)
List Price
$ 1,143.56

Nanosep® Centrifugal Devices with Omega™ Membrane

3K, gray (24/pkg)
List Price
$ 93.61
3K, gray (100/pkg)
List Price
$ 317.37
3K, gray (500/pkg)
List Price
$ 1,332.14
10K, blue (24/pkg)
List Price
$ 93.61
10K, blue (100/pkg)
List Price
$ 317.39
10K, blue (500/pkg)
List Price
$ 1,335.21
30K, red (24/pkg)
List Price
$ 93.61
List Price
$ 317.37
30K, red (500/pkg)
List Price
$ 1,332.14
100K, clear (24/pkg)
List Price
$ 94.30
List Price
$ 317.37
100K, clear (500/pkg)
List Price
$ 1,332.14
300K, orange (24/pkg)
List Price
$ 94.30
300K, orange (100/pkg)
List Price
$ 317.37
300K, orange (500/pkg)
List Price
$ 1,332.14
Description

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

  • Ensures rapid processing of samples 
  • Typical recoveries are > 90%
  • Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
  • A wide range of MWCOs, color-coded for easy identification
  • Constructed of low-binding polypropylene 
  • Ultrasonically welded seals prevent bypass or seal failure
  • Fits standard centrifuge rotors that accept 1.5 mL tubes
Applications
  • Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
  • Clean up labeling and PCR reactions
  • Isolate DNA from agarose gel slices
  • Separate oligonucleotides and RNA from acrylamide gels
  • Concentrate PCR products regardless of size with 30K device if primer removal is required
Specifications

Materials of Construction

  • Nanosep Devices
       Filter Media: Omega (modified polyethersulfone) ultrafiltration membrane
       Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene
  • Nanosep MF Devices
       Filter Media: Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
       Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene

Effective Filtration Area

  • 0.3 cm2

Dimensions

  • Overall Length (Fully Assembled with Cap): 4.5 cm (1.8 in.)

Capacities

  • Maximum Sample Volume: 500 µL
  • Final Concentrate Volume: 15 µL
  • Filtrate Receiver Volume: 500 µL
  • Hold-up Volume (Membrane/Support): < 5 µL

Operating Temperature Range

  • 0 - 40 °C (32 - 104 °F)

pH Range

  • Nanosep Devices: 1 - 14
  • Nanosep MF Devices: 3 - 14

Maximum Centrifugal Force

  • 14,000 x g

Centrifuge

  • Fits rotors that accept 1.5 mL tubes

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through the device prior to use.
Performance

DNA Recovery as a Function of Device MWCO


Nanosep Centrifugal Device: DNA Recovery as a Function of Device MWCO
 
A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL. Recovered samples were quantitated using absorbance at 260 nm. The 100K device was able to differentiate between the sizes of the DNA fragments.

Omega™ Membrane Has the Lowest Non-Specific DNA Binding


Omega™ Membrane Has the Lowest Non-Specific DNA Binding

A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device. The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8). The remaining radioactivity in the device was counted in a scintillation counter. A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample. Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries. In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule. Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g. Otherwise, DNA can be forced through many MWCO membranes regardless of size.

Centrifugal Device Spin Times


Nanosep Centrifugal Device Spin Times
Type
Centrifugal Device
Use
Ultrafiltration, Microfiltration
Application
HPLC/UHPLC Sample Preparation
Segment
Analytical QC
DNA & RNA Purification
Protein Sample Prep & Detection