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AcroPrep Advance Filter Plates for Lysate Clearance
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Download:Description
Prefilter Provides Efficient Clarification
of Highly Particulated Samples
- Integrated prefilter yields consistent filtration of samples with high levels of gross particulate
- New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume
- Innovative outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross-contamination
- Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules
Specifications
Materials of Construction
- Filter Media: 3 μm glass fiber/0.2 μm Supor® membrane and 3 μm glass fiber/1.2 μm Supor membrane
- Plate Housing: Polyproylene
- Lid: Polystyrene
Dimensions
- Length: 12.8 cm (5.0 in.)
- Width: 8.6 cm (3.4 in.)
- Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
- Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.); 2 mL: 4.7 cm (1.9 in.)
Well-Bottom Area
- 0.25 cm2
Recommended Working Volume
- 350 μL: < 300 μL
- 1 mL: < 900 μL
- 2 mL: < 1.9 mL
Recommended Operating Vacuum
- > 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
- 1,500 x g
Typical Processing Time
- PN 8040
- Vacuum: 2 seconds
- Centrifuge: < 2 minutes
- PN 8075, 8175, 8275
- Vacuum: 9 seconds
- Centrifuge: < 2 minutes
Typical Hold-Up Volume
- PN 8040 - Vacuum: 17 μL
- PN 8075, 8175, 8275 - Vacuum: 13 μL
Applications
- Removal of bacterial debris prior to plasmid purification
- Removal of bacterial and cellular debris prior to protein purification
- Clearance of gross particulates
Performance
Glass Fiber and Supor Membrane Allow Complete Recovery of Plasmid DNA from E. coli Lysates
Purified pCAT plasmid DNA was spiked in TE buffer at 25 μg/mL, 50 μg/mL, and 100 μg/mL and filtered through Pall and Competitor A’s prefilter plates. Percent recovery was calculated by comparing OD260 post-filtration to a standard curve of unfiltered samples.
Type
Filter Plates
Product Summary
AcroPrep™ Advance Filter Plates for Lysate Clearance are manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules.
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