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BioTrace™ NT Nitrocellulose Transfer Membrane
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BioTrace NT Nitr°Cellulose Transfer Membrane
Description
Pure, Unsupported Nitrocellulose Membrane for Nucleic Acid and Protein Detection
- High binding capacity for proteins and nucleic acids
- Lower protein burnthrough than competitors in electrophoretic transfers
Applications
- Protein transfers
- Western blots
- Southern blots
- Northern blots
- Nucleic acid and protein dot blots
Specifications
Filter Media
- BioTrace NT (nitrocellulose)
Pore Size
- 0.2 μm
Typical Thickness
- 101.6 - 190.5 μm (4.0 - 7.5 mils)
Performance

Less Burnthrough with Nitrocellulose Membranes

Prestained proteins were separated in a polyacrylamide gel and electrophoretically transferred to the indicated nitrocellulose membranes. A double layer of membrane was used, one directly against the gel, followed by the second layer. Signal intensity on the second layer is indicative of burnthrough, which can lead to loss of signal.
Use
Binding
Application
Northern & Southern Blotting
Product Summary
Membranes have a high binding affinity (209µg/cm2) and are ideal for Western blot confirmatory tests. High binding capacity for proteins and nucleic acids. Lower protein burnthrough than competitors in electrophoretic transfers.