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AcroPrep Advance 96-Well Filter Plates With Mustang Q/S Ion Exchange Membrane
AcroPrep Advance Filter Plates for Protein purification
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Adapter collar for centrifugation (2/pkg)
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Cap mat for incubation (5/pkg)
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Description
Versatile Format for Multiple
Versatile Format for Multiple
Purification Strategies
- High performance Supor® membrane offers optimal support to retain chromatography sorbents while allowing smooth flow of buffers
- Mustang® membrane is able to withstand high flow rates to render fast purification of biomolecules
- New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume
- New outlet tip geometry minimizes sample leakage and loss during incubation steps
- Intrinsic plate properties prevent target molecules from binding to the plate
Applications
- High throughput protein purification
- Screening of chromatography sorbents
- Screening of chromatography conditions
- Protein fractionation
- Antibody purification
Specifications
Materials of Construction
- Filter Media: Supor (polyethersulfone), Mustang Q (anion exchange), and Mustang S (cation exchange) membranes
- Plate Housing: Polyproylene
- Lid: Polystyrene
Dimensions
- Length: 12.8 cm (5.0 in.)
- Width: 8.6 cm (3.4 in.)
- Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
- Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.)
Well-Bottom Area
- 0.25 cm2
Recommended Working Volume
- 350 μL: < 300 μL
- 1 mL: < 900 μL
Recommended Operating Vacuum
- > 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
- 1,500 x g
Typical Processing Time
- PN 8029, 8039, 8129, 8130
- Vacuum: 2 seconds
- Centrifuge: < 2 minutes
- PN 8071, 8072, 8171, 8172
- Vacuum: 14 seconds
- Centrifuge: < 2 minutes
Typical Hold-Up Volume
- PN 8029, 8039, 8129, 8130
- Vacuum: 5 μL
- Centrifuge: 3 μL
- PN 8071, 8072, 8171, 8172
- Vacuum: 21 μL
- Centrifuge: 12 μL
Performance
Mustang Q and S Membrane Provide a High Protein Binding Capacity
Binding capacity of Mustang Q membrane, 0.38 mg/well (n=5), was determined with BSA in 50 mM Tris, pH 8.5. Mustang S membrane binding capacity, 0.29 mg/well (n=6), was determined using Lysozyme in 10 mM MES, pH 5.5.
Type
Filter Plates
Use
Protein Purification, High throughput filtration
Application
Protein Fractionation
Product Summary
The AcroPrep™ Advance filter plate portfolio includes a variety of membrane types and pore sizes to provide the best possible performance for a wide range of sample preparation and high throughput screening applications.
