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MEP HyperCel™ Mixed-Mode Chromatography Sorbent product photo Primary L

MEP HyperCel™ Mixed-Mode Chromatography Sorbent

For the Capture and Purification of
Monoclonal and Polyclonal Antibodies

  • High purity in a single step - Product purities of 70% to 90% or greater are typically achieved
  • Rapid sample processing - Dynamic binding capacities of > 30 mg IgG per mL of sorbent (at 10% breakthrough) are routinely achieved
  • Exhibits high IgG capacity, independent of sub-class or species
  • Allows direct sample loading without adjustment of pH or ionic strength
  • Effective capture, even with feedstocks as dilute as 50 to 100 μg IgG/mL
  • Offers easy cleaning with sodium hydroxide

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."


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MEP HyperCel Hydrophobic Charge Induction Chromatography (HCIC) Resin

List Price
$ 3,680.00

Sorbent

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$ 520.00
SDR HyperD (25 mL)
List Price
$ 120.00
List Price
$ 140.00
Description

For the Capture and Purification of
Monoclonal and Polyclonal Antibodies

  • High purity in a single step - Product purities of 70% to 90% or greater are typically achieved
  • Rapid sample processing - Dynamic binding capacities of > 30 mg IgG per mL of sorbent (at 10% breakthrough) are routinely achieved
  • Exhibits high IgG capacity, independent of sub-class or species
  • Allows direct sample loading without adjustment of pH or ionic strength
  • Effective capture, even with feedstocks as dilute as 50 to 100 μg IgG/mL
  • Offers easy cleaning with sodium hydroxide

“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."
Applications
  • Capture and purification of antibodies from a broad range of sources such as animal sera, ascites fluid, and cell culture supernatant.
  • Independent of subclass or species. Weakly-binding variants (e.g., murine IgG, rat IgG) are well retained.
  • Alternative to traditional HIC sorbents for capture of hydrophobic molecules.
  • Has been studied in a broad range of applications including isolation of antibodies from sweet-whey and colostrum; isolation of antibodies from transgenic plant and animal sources; and, isolation of IgA and selected fusion proteins
Specifications

Particle Size

  • 80 - 100 µm (average)

Bead Composition

  • High porosity cross-linked cellulose

Dynamic Binding Capacity for Human IgG (10% breakthrough)1

  • > 20 mg/mL

Ligand

  • 4-Mercaptoethylpyridine (4-MEP)

Ligand Density

  • 80 - 125 µmol/mL

Working pH

  • 2 - 12

Pressure Resistance

  • < 3 bar (300 kPa, 44 psi)

Storage Temperature

  • 2 - 8 °C (36 - 46 °F)

1Determined using 5 mg/mL human IgG in PBS, flow rate 60 cm/h.
Performance

Monoclonal Antibodies Purification on MEP
HyperCel Resin from Cell Culture Supernatants

Panel A: Serum-free Cell Culture Media 

Monoclonal Antibodies Purification on MEP HyperCel Resin from Cell Culture Supernatants

Panel B: 5% Serum-supplemented Cell Culture Media

Monoclonal Antibodies Purification on MEP HyperCel Resin from Cell Culture Supernatants

Sample A = 300 mL protein-free cell culture supernatant. Sample B = 300 mL cell culture supernatant containing 5% fetal bovine serum; Equilibration: 50 mM Tris-HCI, pH 8; Elution: 50 mM acetate, pH4; Flow rate: 70 cm/h. In curve B, (a) and (b) are respectively water and 25 mM sodium caprylate washings; SDS-PAGE (reduced conditions): (1) = crude sample; (2) = purified IgG; (H) = heavy chain; (L) = light chain.
Type
Chromatography Sorbent
Use
Mixed-mode Chromatography
Application
Chromatography