Centramate Cassettes with Supor Membrane - Suspended Screen
Centramate Fitting Packages
Holders and Accessories
Suitable for Process Development and
Small-Scale Production of 1 to 125 L
- Filtration area is easily expanded by adding additional membrane cassettes
- Identical fluid path lengths provide precise linear scale-up to larger process systems available from Pall Corporation
- Cassettes can be easily removed without disassembling system plumbing
- Systems are available with Type 316L stainless steel (Centramate system) or economically priced, extremely durable, ultra-high molecular weight polyethylene (Centramate PE system)
- Variety of membrane types provide versatility to the systems
Materials of Construction
- Membrane Cassette
- Filter Media: Supor® [hydrophilic polyethersulfone (PES)], Omega™ (low protein-binding, modified PES) membranes
- Encapsulant: Polyurethane
- Gasket: Silicone
- Cassette Hardware
- Wetted Parts:
- Centramate Systems: Type 316L stainless steel
- Centramate PE Systems Ultra-high molecular weight polyethylene
- Tie Rods: Stainless steel
- Nuts: Bronze
- Centramate Systems: 13.2 x 22.6 x 16.7 cm (5.2 x 8.9 x 6.6 in.)
- Centramate PE Systems: 9.4 x 25.1 x 19.3 cm (3.7 x 9.4 x 7.6 in.)
Typical Hold-Up Volume
- < 20 mL (per cassette)
Piping Connections (Feed, Retentate, and Filtrate)
- Centramate Systems: 12.7 mm (1/2 in.) sanitary connections
- Centramate PE Systems: 6.4 mm (1/4 in.) pipe
Maximum Operating Temperature (Hardware)
- Centramate Systems: 121 °C (250 °F)
- Centramate PE Systems: 70 °C (158 °F) Cassettes: 60 °C (140 °F)
Recommended Operating Pressure
- 0.3 bar (30 kPa, 5 psi) – 5.2 bar (520 kPa, 75 psi)
- Centramate Systems: 7.7 kg (17 lb.)
- Centramate PE Systems: 6.4 kg (14 lb.)
Typical Cross Flow Rate Per 0.09 m2 (1.0 ft2) Cassette (L/min)
- Screen: 0.6 - 0.8
- Suspended Screen: 1.0 - 2.0
Screen Selection Guideline
- Screen for optimized processing of particle-free protein solutions to high concentrations
- Suspended screen for light to moderate particle load solutions
- Perform laboratory trials and pilot scale processing
- Concentration of biomolecules
- Recovery and purification of biomolecules
- Diafiltration and desalting
- Fractionation of mixed molecule solutions
1 Q. How many times can a Minimate™ capsules be reused?
A. There are no specific guidelines for the total number of times a Minimate capsule can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Minimate capsules prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of capsules should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Minimate capsule drops below 50% of the original NWP value, it is suggested that a new capsule is used.
2 Q. How many times can a Centramate™ cassettes be reused?
A. There are no specific guidelines for the total number of times a Centramate cassette can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Centramate cassettes prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of cassettes should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Centramate cassette drops below 50% of the original NWP value, it is suggested that a new cassette is used.
3 Q. When should I order Centramate™ vs Centramate PE?
A. Certain applications require a fluid path that is free from metal. Our standard Centramate has stainless steel fittings and a 100% stainless steel cassette holder, so it would not be a good option for these applications. Pall’s Centramate PE has plastic fittings and the fluid path of the holder is polyethylene plastic. The pressure gauge that comes standard with the Centramate PE will have a stainless steel pathway, but the ¼” NPT fittings the system uses makes it easy to use a pressure gauge whose wettable parts will be free from metal.
4 Q. What do I need to attach an LV Centramate™ to the Minimate™ EVO system?
A. Pall recommends ordering the Minimate EVO Fittings kit, PN 97014, to get the additional 3-way stop cocks, hose barb adapters, and tubing needed to properly connect the LV Centramate holder to the Minimate EVO system. Contact your Pall representative or technical support if you have further questions.
5 Q. How many cassettes can I stack in a Centramate™ or Centramate PE?
A. You can stack up to 5 0.1 m2 T12 cassettes on Centramate, giving you up to 0.5m2 of effective filtration area. For the Centramate PE, only 4 0.1 m2 T12 cassettes will fit because of the thicker PE base. Stacking multiple cassettes in the holders increases the surface area of the membrane which gives a higher permeate flow rate and increases the throughput.
6 Q. Guidance on average recirculation flow rates and permeate flux rates for the Minimate™, LV Centramate™, Centramate PE/SS.
A. For the Minimate we recommend a recirculation flow rate of 30-40 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 4 ml/min. For the LV Centramate we recommend a recirculation flow rate of 120-160 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 15 ml/min. For the Centramate and Centramate PE we recommend a recirculation flow rate of 600-800 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 75 ml/min. Note: These are typical values. Actual values may be higher or lower depending on the membr