Free ground shipping on Laboratory products for registered Credit Card users.
Biodyne® Nylon Transfer Membranes product photo Primary L

Biodyne® Nylon Transfer Membranes

High Sensitivity and Low Background
for Enhanced Detection and Resolution

  • Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
  • Membranes are intrinsically hydrophilic for easy wetting
  • Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."


Need Help?
Ask a Question
* Required

Order Products

Biodyne A Membrane

Biodyne A Membrane - 0.2 µm, 30 cm x 3 m roll (1/pkg)

Product ID: 60113
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis|Chemiluminescence
Filter Media
Amphoteric Nylon 6,6
Minimum Typical Thickness (Metric µm)
5.5 µm
Maximum Typical Thickness (Metric µm)
7 µm
Pore Size (Removal Rating)
0.2 µm
Roll Size Length (Metric cm)
30 cm
See All Attributes

Biodyne A Membrane - 0.45 µm, 30 cm x 3 m roll (1/pkg)

Product ID: 60106
Unit of Measure
1/EA
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis|Chemiluminescence
Filter Media
Amphoteric Nylon 6,6
Minimum Typical Thickness (Metric µm)
5.5 µm
Maximum Typical Thickness (Metric µm)
7 µm
Pore Size (Removal Rating)
0.45 µm
Roll Size Length (Metric cm)
30 cm
See All Attributes

Biodyne B Membrane, 0.45 µm

Biodyne B Membrane, 0.45 µm - 20 cm x 3 m roll (1/pkg)

Product ID: 60208
Unit of Measure
1/ROL
Min Order Qty
1
Detection System
radioactive, chromogenic, chemiluminescence, fluorescence
Filter Media
Positively-charged Nylon 6,6
Max Typical Thickness
6.7 µm
Min Typical Thickness
5.7 µm
Pore Size
0.45 μm
Roll Size Length (Metric)
20 m
See All Attributes

Biodyne B Membrane, 0.45 µm - 30 cm x 3 m roll (1/pkg)

Product ID: 60207
Unit of Measure
1/EA
Min Order Qty
1
Detection System
radioactive, chromogenic, chemiluminescence, fluorescence
Filter Media
Positively-charged Nylon 6,6
Max Typical Thickness
6.7 µm
Min Typical Thickness
5.7 µm
Pore Size
0.45 μm
Roll Size Length (Metric)
30 m
See All Attributes

Biodyne B Membrane, 0.45 µm - 20 x 20 cm sheets (10/pkg)

Product ID: 60200
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
radioactive, chromogenic, chemiluminescence, fluorescence
Filter Media
Positively-charged Nylon 6,6
Max Typical Thickness
6.7 µm
Min Typical Thickness
5.7 µm
Pore Size
0.45 μm
Sheet Size Length (Metric)
20 cm
See All Attributes

Biodyne B Membrane, 0.45 µm - 7 x 8.5 cm sheets (10/pkg)

Product ID: 60201
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
radioactive, chromogenic, chemiluminescence, fluorescence
Filter Media
Positively-charged Nylon 6,6
Max Typical Thickness
6.7 µm
Min Typical Thickness
5.7 µm
Pore Size
0.45 μm
Sheet Size Length (Metric)
7 cm
See All Attributes

Biodyne C Membrane, 0.45 µm

Biodyne C Membrane, 0.45 µm - 20 x 20 cm sheets (10/pkg)

Product ID: 60314
Unit of Measure
1/EA
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis
Filter Media
Negativelycharged Nylon 6,6
Minimum Typical Thickness (Metric µm)
11 µm
Maximum Typical Thickness (Metric µm)
13 µm
Pore Size (Removal Rating)
0.45 µm
Sheet Size Length (Metric cm)
20 cm
See All Attributes

Biodyne C Membrane, 0.45 µm - 7 x 8.5 cm sheets (10/pkg)

Product ID: 60315
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis
Filter Media
Negativelycharged Nylon 6,6
Minimum Typical Thickness (Metric µm)
11 µm
Maximum Typical Thickness (Metric µm)
13 µm
Pore Size (Removal Rating)
0.45 µm
Sheet Size Length (Metric cm)
7 cm
See All Attributes

Biodyne C Membrane, 0.45 µm - 137 mm discs (50/pkg)

Product ID: 60319
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis
Disc Size (Metric mm)
137 mm
Filter Media
Negativelycharged Nylon 6,6
Minimum Typical Thickness (Metric µm)
11 µm
Maximum Typical Thickness (Metric µm)
13 µm
Pore Size (Removal Rating)
0.45 µm
See All Attributes

Biodyne Plus Membrane, 0.45 µm

Biodyne Plus Membrane, 0.45 µm - 30 cm x 3 m roll (1/pkg)

Product ID: 60406
Unit of Measure
1/ROL
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis|Chemiluminescence|Fluorescence
Filter Media
Positively-charged Nylon 6,6
Minimum Typical Thickness (Metric µm)
5.7 µm
Maximum Typical Thickness (Metric µm)
6.7 µm
Pore Size (Removal Rating)
0.45 µm
Roll Size Length (Metric cm)
30 cm
See All Attributes

Biodyne Plus Membrane, 0.45 µm - 132 mm discs (50/pkg)

Product ID: 60404
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis|Chemiluminescence|Fluorescence
Disc Size (Metric mm)
132 mm
Filter Media
Negativelycharged Nylon 6,6
Minimum Typical Thickness (Metric µm)
5.7 µm
Maximum Typical Thickness (Metric µm)
6.7 µm
Pore Size (Removal Rating)
0.45 µm
See All Attributes

Biodyne Plus Membrane, 0.45 µm - 20 x 20 cm sheets (10/pkg)

Product ID: 60400
Unit of Measure
1/PAC
Min Order Qty
1
Detection System
Radioactivity|Chromogenesis|Chemiluminescence|Fluorescence
Filter Media
Negativelycharged Nylon 6,6
Minimum Typical Thickness (Metric µm)
5.7 µm
Maximum Typical Thickness (Metric µm)
6.7 µm
Pore Size (Removal Rating)
0.45 µm
Sheet Size Length (Metric cm)
20 cm
See All Attributes

Documents

Datasheets

Download: English

Description

High Sensitivity and Low Background
for Enhanced Detection and Resolution

  • Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
  • Membranes are intrinsically hydrophilic for easy wetting
  • Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please email us a question or call 1-800-521-1520."

Specifications

Filter Media

  • Biodyne A Membrane: Amphoteric Nylon 6,6
  • Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
  • Biodyne C Membrane: Negativelycharged Nylon 6,6

Pore Size

  • 0.2, 0.45, and 1.2 μm

Typical Thickness

 
Membrane μm mils
Biodyne A 5.5 - 7.0 139.7 - 177.8
Biodyne B 5.7 - 6.7 144.8 - 170.2
Biodyne Plus 5.7 - 6.7 144.8 - 170.2
Biodyne C 11.0 - 13.01 279.4 - 330.2
 

Solvent Compatibility

  • Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatible with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

1Dual layer measurements

Applications

Four Chemistries Provide Versatile Adsorption Properties

Biodyne A Membrane

  • (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

Biodyne B Membrane

  • (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.

Biodyne C Membrane

  • (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

Biodyne Plus Membrane

  • (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

Performance

Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing


Biodyne B Membrane Withstands Stripping and Reprobing
Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 

1A - 4A: blot after (re)probing
1B - 4B: blot after stripping, prior to re(probing)

Fluorescent Detection of DNA Using Biodyne Plus Membrane


Fluorescent Detection of DNA Using Biodyne Plus Membrane
ng total DNA

Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.

Type

Membrane Filters

Use

Binding

Product Summary

Provide high sensitivity and low background for enhanced detection and resolution. Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing.