Chromatography Resin product photo Primary L

Chromatography Resin

For Biomolecule Isolation

   

Chromatography continues to be an essential technology for the purification of biomolecules. In chromatography, a complex sample is passed through a chromatographic matrix that consists of a solid support with specific characteristics defined by the support's make-up and/or chemistry. The type of support chosen will depend on the nature of the purification desired. Pall offers a competitive portfolio of resins for affinity, ion exchange and hydroxyapatite chromatography. Our resins can be incorporated into devices to cover a variety of research needs or they can be used in conjunction with one of our microporous membranes for diagnostic test development.

When performing chromatography-based purification, the choice of resin is crucial for ensuring effective capture (binding selectivity/capacity) and sufficient separation (resolution). Pall offers a variety of base materials ranging from soft beads to rigid beads to a unique hybrid bead. Our hybrid beads are created using Pall's patented "gel-in-a-shell" technology. This "gel-in-a-shell" bead is constructed of a high-capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. The capture chemistry, or ligand, is attached to the hydrogel within the bead pores.

 Gel-in-a-Shell Technology

 

Using enhanced diffusion, Pall's "gel-in-a-shell" technology binds product throughout the gel-filled pore, enhancing total binding capacity.

The advantages of this structure are many, including higher than usual capacity, tolerance to fast chromatography runs without the usual increase in back pressure, and greater salt tolerance for ion exchangers. By tailoring attributes to specific applications, Pall chromatography resins exhibit the highest performance characteristics possible while ensuring reliable, reproducible biomolecule isolation.



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Description

For Biomolecule Isolation

   

Chromatography continues to be an essential technology for the purification of biomolecules. In chromatography, a complex sample is passed through a chromatographic matrix that consists of a solid support with specific characteristics defined by the support's make-up and/or chemistry. The type of support chosen will depend on the nature of the purification desired. Pall offers a competitive portfolio of resins for affinity, ion exchange and hydroxyapatite chromatography. Our resins can be incorporated into devices to cover a variety of research needs or they can be used in conjunction with one of our microporous membranes for diagnostic test development.

When performing chromatography-based purification, the choice of resin is crucial for ensuring effective capture (binding selectivity/capacity) and sufficient separation (resolution). Pall offers a variety of base materials ranging from soft beads to rigid beads to a unique hybrid bead. Our hybrid beads are created using Pall's patented "gel-in-a-shell" technology. This "gel-in-a-shell" bead is constructed of a high-capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. The capture chemistry, or ligand, is attached to the hydrogel within the bead pores.

 Gel-in-a-Shell Technology

 

Using enhanced diffusion, Pall's "gel-in-a-shell" technology binds product throughout the gel-filled pore, enhancing total binding capacity.

The advantages of this structure are many, including higher than usual capacity, tolerance to fast chromatography runs without the usual increase in back pressure, and greater salt tolerance for ion exchangers. By tailoring attributes to specific applications, Pall chromatography resins exhibit the highest performance characteristics possible while ensuring reliable, reproducible biomolecule isolation.

Applications

Applications

  • Affinity removal
  • Ion exchange
  • Gel filtration 
 
Description Typical Applications
Ceramic HyperD F (Q, DEAE, and CM chemistry) Biomolecule concentration, protein separation, contaminant removal
Blue Trisacryl M Albumin depletion
Heparin HyperD M Purification of coagulation factors, lipoproteins, growth hormones, growth factors, nucleic acid binding enzymes
Lysine HyperD Purification of glycoproteins
 
Specifications

Typical Affinity Resin Characteristics

 
Description Particle Size (µm) Ligand Working pH Cleaning pH Pressure Stability (psi) Pressure Stability (bar)
Blue Trisacryl® 40-80 Cibacron Blue 1-10 --- 45 3
Heparin HyperD M 80 Porcine Heparine 3-13 --- 1,000 70
Lysine HyperD 70 L-lysine 3-13 3-13 1,000 70
HA Ultrogel® 60-180 Hydroxyapatite 5-14 --- --- ---
 

Typical Performance Characteristics

 
Description Dynamic Binding Capacity
Blue Trisacryl HSA: 10-15 mg/mL**
BSA: 5-7 mg/mL**
Heparin HyperD M > 25 mg/mL for human ATIII***
Lysine HyperD ---
HA Ultrogel Cytochrome C: > 7 mg/mL****
BSA: < 7 mg/mL****
 
**Capacity determined in PBS using 5 mg/mL.
***Determined using human ATIII at 72.5 IU/mL in 20 mM Tris HCl, 0.3M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2M NaCl, pH 7.4 at a flow rate of 600 cm/hr, 10 cm bed height. ****Capacity for Cytochrome C determined using 5 mg/mL Cytochrome C diluted 50/50 in 1 mM phosphate buffer, pH 6.8, at 12.5 cm/h. Capacity for BSA determined using 1 mg/mL BSA diluted 50/50 in 1 mM phosphate buffer, pH 6.8, at 12.5 cm/h.

Typical Ion Exchange Resin Characteristics

 
Description Particle Size (µm) Chemistry Working pH Cleaning pH Pressure Stability (psi) Pressure Stability (bar)
Q Ceramic HyperD F 50 Strong anion exchange 2-12 1-14 1,000 70
DEAE Ceramic HyperD F 50 Weak anion exchange 2-12 1-14 1,000 70
CM Ceramic HyperD F 50 Weak cation exchange 2-12 1-14 1,000 70
 

Typical Performance Characteristics

 
Description Dynamic Binding Capacity
Q Ceramic HyperD F > 85 mg/mL*
DEAE Ceramic HyperD F > 85 mg/mL*
CM Ceramic HyperD F > 60 mg/mL**
 
*Dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: mg/mL BSA in 50 mM Tris HCl buffer, pH 8.6.

** Dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: mg/mL hu IgG in 50 mM sodium acetate, 100 mM NaCl, pH 7.4.

Performance

Protein Standards Resolved by Ceramic HyperD Ion Exchange Resin Under Linear Gradient Elution Conditions

Panels A, protein mix (0.5 m/mL Trypsinogen [T, pl 9.3], Ovalbumin [0, pl 5.1/5.3] and Beta Lactoglobulin [Lac, pl 4.6] in 25 mM Tris HCl pH 8.5) loaded (0.5 mL) onto 1 mL volume Ceramic HyperD anion ion exchange resins. Elution with a linear gradient up to 50% B (1 M NaCl in loading buffer) at 1 mL/min. Panels B, protein mix (0.5 m/mL Trypsinogen [T, pl 9.8] and Lysozyme [Lys, pl 11.2] in 10 mM MES-NaOH pH 5.8) loaded (0.5 mL) onto 1 mL volume Ceramic HyperD cation ion exchange resins. Elution with a linear gradient up to 50% B (1 M NaCl in loading buffer) at 1 mL/min.

Type
Media
Use
Filtration, Removal
Ordering Information

Additional resins are available in larger volumes. Please contact your local sales representative for additional information.

 
Part Number Description Pkg
25896-S Blue Trisacryl M resin 1 mL
 
Application
Liquid and Aerosol Barrier Protection, Diagnostics - Sample Preparation, Drug Preparation and Compounding, Drug Preparation and Compounding