Ion Exchange Membrane product photo Primary L

Ion Exchange Membrane

For Biomolecule Capture

           

Pall's family of ion exchange membranes utilizes the principles of sorbent-based ion exchange chromatography on flat stock membrane. This provides ease-of-use and handling benefits over resin-based slurries in a manufacturing environment. Our ion exchange membranes deliver efficient and rapid flow rates with a convective pore structure resulting in processing times that are much shorter and more efficient than conventional resin-based technologies even when purifying large molecules such as plasmids and viruses. Optimization of our ion exchange membranes has resulted in throughputs of up to 100 times that of traditional resin-based media with no associated loss of capacity.

Our ion exchange membranes are available in two chemistries: Q and S. The strong anion exchange membrane (Q chemistry) is ideal for the capture/release of plasmid DNA, virus or target proteins from a complex mixture. The Q chemistry is also well suited for purifying oligos and the protein polishing of negatively-charged proteins. The strong cation exchange membrane (S chemistry) purifies and concentrates positively-charged proteins and viral particles.

Note: The capture of biomolecules is typically done in the presence of low salt. Due to the nature of our ion exchange membranes, they are more salt sensitive than the same chemistries on beads. This should be considered when choosing the buffers for capture and elution.



* Required
Description

For Biomolecule Capture

           

Pall's family of ion exchange membranes utilizes the principles of sorbent-based ion exchange chromatography on flat stock membrane. This provides ease-of-use and handling benefits over resin-based slurries in a manufacturing environment. Our ion exchange membranes deliver efficient and rapid flow rates with a convective pore structure resulting in processing times that are much shorter and more efficient than conventional resin-based technologies even when purifying large molecules such as plasmids and viruses. Optimization of our ion exchange membranes has resulted in throughputs of up to 100 times that of traditional resin-based media with no associated loss of capacity.

Our ion exchange membranes are available in two chemistries: Q and S. The strong anion exchange membrane (Q chemistry) is ideal for the capture/release of plasmid DNA, virus or target proteins from a complex mixture. The Q chemistry is also well suited for purifying oligos and the protein polishing of negatively-charged proteins. The strong cation exchange membrane (S chemistry) purifies and concentrates positively-charged proteins and viral particles.

Note: The capture of biomolecules is typically done in the presence of low salt. Due to the nature of our ion exchange membranes, they are more salt sensitive than the same chemistries on beads. This should be considered when choosing the buffers for capture and elution.

Applications

Applications

  • Biomolecule capture
  • Plasmid preparation

Sealing

  • Mechanical
  • Heat
  • Ultrasonic
  • RF welding
  • Insert molding
Specifications

Typical Membrane Characteristics

Base Material Thickness (mils) Thickness (µm)
Anionic Exchange (Q) 4.7-6.3 119.4-160.0
Cationic exchange (S) 4.5-6.3 114.3-160.0

 

Typical Performance Characteristics

Base Material Dynamic DNA Binding Capacity (mg/mL) Water Bubble Point (psi) Water Bubble Point (bar) Dynamic Protein Binding (mg/mL) Water Flow (mL/min/cm2 @ 0.7 bar, 10 psi)
Anionic Exchange (Q) > 12.0 N/A N/A > 30.0 30.0-140.0
Cationic exchange (S) N/A N/A N/A > 30.0 > 36.0
Performance

Acrodisc® Unit with Anion Exchange (Q) Membrane: Dynamic Binding with BSA

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1 minutes and was calculated as 54 mg/mL using:
Flow Rate (2.3 mL/min) X Initial Protein BSA Concentration (0.524 mg/mL) X time (8.1 min)
---------------------------------------------------------------------------------------------------------------------------
Membrane Bed Volume of Anion Exchange Membrane in 25 mm Acrodisc Unit (0.18 mL)

The Acrodisc name is a registered trademark of Pall Corporation and is not available for use.

Type
Media, Membranes and Materials
Use
Filtration, Separation, Removal
Ordering Information

Custom roll, sheet, and disc sizes available upon request. Please contact your local sales representative for additional information.

Part NumberDescriptionPkg
S80574 Mustang Anionic Exchange (Q) membrane, 8" x 10" sheet 1/pkg
S80570 Mustang Cationic Exchange (S) membrane, 8" x 10" sheet 1/pkg

The Mustang name is a registered trademark of Pall Corporation and is not available for use.
Products in this datasheet may be covered by one or more patents including EP 1,163, 045; EP 1,614,459; US 6,780,327; US 6,851,561; US 7,094,347; US 7,223,341; EP 1,163,044; US 6,783,937,US 6,860,393; and US 7,132,049.

Application
Diagnostics - Sample Preparation
Segment
Advanced Materials
Diagnostics
OEM Manufacturing