24-well Filter Plates

Pall supplies a comprehensive range of 24-well, 7 mL, filter plates that offer researchers a complete solution for their workflow needs, from cell harvesting to final sample preparation for analysis. There is no longer the need for customers to change formats, now they can stay in 24-well format and reduce the chance of errors or the loss of valuable product.

AcroPrepTM 24-well filter plates are available in the widest variety of membrane options allowing customers the best choice of 24 multi-well filter plate based on their application and pore size requirements.

AcroPrep 24-well Filter Plates

Product images are a representation of product family for illustration purposes only. Actual products may vary.

Order Products

Applications

Filter Plates

Segments

Sterile Filtration and Clarification

AcroPrep 24-well Cell Clarification and Sterile Filtration Plate-Depth filter plus 0.65/0.2 µm Supor EKV (8/pkg)

Product ID: 97026
Unit of Measure
1/PAC
Min Order Qty
1
EFA (Effective Filtration Area)
1.6 cm²
Sample Volume
7 mL
Height (Metric cm)
3.9 cm
Length (Metric cm)
12.8 cm
Width (Metric)
8.6 cm
Plate Housing Material
polypropylene
See All Attributes

AcroPrep 24-well Sterile Filtration Plate-0.65/0.2 μm Supor EKV (2/pkg)

Product ID: 97017
Unit of Measure
1/PAC
Min Order Qty
1
EFA (Effective Filtration Area)
1.6 cm²
Filter Media
Supor EKV (Hydrophilic polyethersulfone)
Sample Volume
7 mL
Height (Metric cm)
3.9 cm
Length (Metric cm)
12.8 cm
Width (Metric)
8.6 cm
See All Attributes

AcroPrep 24-well Cell Clarification and Sterile Filtration Plate-Depth filter plus 0.65/0.2 µm Supor EKV (2/pkg)

Product ID: 97016
Unit of Measure
1/PAC
Min Order Qty
1
EFA (Effective Filtration Area)
1.6 cm²
Sample Volume
7 mL
Height (Metric cm)
3.9 cm
Length (Metric cm)
12.8 cm
Width (Metric)
8.6 cm
Plate Housing Material
polypropylene
See All Attributes

AcroPrep 24-well Sterile Filtration Plate-0.65/0.2 μm Supor EKV (8/pkg)

Product ID: 97027
Unit of Measure
1/PAC
Min Order Qty
1
EFA (Effective Filtration Area)
1.6 cm²
Filter Media
Supor EKV (Hydrophilic polyethersulfone)
Sample Volume
7 mL
Height (Metric cm)
3.9 cm
Length (Metric cm)
12.8 cm
Width (Metric)
8.6 cm
See All Attributes

Description

Pall’s AcroPrepTM 24-well filter plates feature a range of Pall’s proprietary membranes to meet the requirements of researcher’s applications and workflows. The filter plates feature a ridged and robust polypropylene construction that offers extensive drug and chemical compatibility. The low protein binding and low extractable properties of both filter plate and membrane materials ensure maximum recovery of valuable samples.

 

Utilizing Pall’s proprietary high-performance multi-layer filter media and membranes, these plates offer time savings, strong performance claims and streamlined workflow improvements in a 24-well plate format.

 

  • Intrinsic plate and membrane properties minimize sample loss from non-specific binding 
  • Automation friendly – compatible with all major automation platforms 
  • Designed in accordance with the ANSI/SLAS X-2004 standards 
  • Vacuum compatible – compatible with all popular vacuum and positive pressure manifolds 
  • Centrifugation - suitable for centrifugation with compatible rotors

AcroPrep Cell Clarification & Sterile Filtration Plate Accelerating Protein Purification Workflows

 

AcroPrep Cell Clarification and Sterile Filtration Plate Accelerating Protein Purification Workflows

 
Pall’s clarification and sterile filtration AcroPrep 24-well filter plate can both clarify and sterile filter in a single device and in a single workflow step. Each of the 24 wells contains Pall Seitz® depth media layered over a 0.65/0.2 μm Supor® EKV  membrane. With either a vacuum manifold or centrifuge, high density cell cultures (such as CHO or HEK) can be quickly processed resulting in the capture  of cells, cell debris and other biological aggregates in  the filter media. The filtrate collected by the 24-well collection plate only contains proteins and other sub 0.2 μm particles. 
 
By using this multi-layer filtration media and membrane design integrated into one device, it is possible to effortlessly recover proteins from whole cell cultures with varying variabilities of up to 25 M cells/mL.
 
A traditional multistep method for 24-well culture harvesting would involve the use of tubes, tips, filters and syringes with each transfer step, which can increase the chance of contamination and potential product loss, as well as being labor intensive. Combining the clarification and sterilization steps eliminates the need to harvest the cells in a centrifugation step, saving time and simplifying workflows. 
 
  • Offers workflow that is compatible with 24-well cell culture formats
  • Depth filter plus 0.65/0.2 µm Supor EKV membrane 
  • Demonstrates retention of 107 cfu/cm2 of B. diminuta per modified ASTM F838 
  • Depth media allows clarification of large cellular debris prior to filtration through the 0.65/0.2 µm Supor EKV membrane 
  • Reliable recovery of > 90% of extra cellular proteins
  • Uses up to seven times (7x) less plastic consumables by weight, significantly reducing disposal costs compared with traditional methods

Sterile Filtration Workflows with the AcroPrep 0.2 μm Supor EKV Filtration Plate

Pall’s sterile filtration AcroPrep 24-well filter plate is well suited for the high volume (up to 7 mL) plate-based sterile filtration of media, reagents, serum or protein. The filter plate features a dual-layer, 0.65 μm membrane integrated with a highly asymmetric 0.2 μm membrane, this offers a fast, efficient, and sterilizing grade filtration.

 

Unlike alternative sterilizing grade filter plates available on the market, the Pall AcroPrep 0.2 μm Supor EKV Filtration Plates are individually bagged and gamma irradiated.

 

  • Offers workflow that is compatible with 24-well cell culture formats
  • 0.65/0.2 µm Supor EKV dual-layer membrane
  • Process up to 7 mL of media, reagents, serum or protein in sterile filtrate applications
  • Compatible for use with either a vacuum manifold or centrifuge
  • Sterilizing grade plate, demonstrates retention of 107 cfu/cm2 of B. diminuta per modified ASTM F838 
  • Gamma irradiated and individual bagged (both filter and collection plate)

AcroPrep 24-well Filter Plates with Supor Membrane

AcropPrep 24-well filter plates with Supor membrane can be used for a variety of applications. The Supor polyethersulfone (PES) membrane enables fast filtration, has low protein binding properties and offers extensive drug and chemical compatibility.

 

Available with 0.1, 0.45, 0.8, 1.2 and 5 μm pore size ratings, these versatile filter plates can be confidently incorporated into a number of laboratory 24- well format workflows. 

 

  • Protein purification: High performance Supor membrane offers optimal support to retain chromatography sorbents while allowing smooth flow of buffers
  • Lysate clearance and general sample preparation: Supor membrane is ideal for particulate removal or lysate clearance prior to downstream analysis or subsequent purifications 
  • Multiplexing assays: These high-performance membranes do not trap microspheres in the membrane matrix, ensuring superior bead recovery and reducing false positives
  • Aqueous filtration: The broad drug and chemical compatibility of the Supor membrane make it ideal for filtering aqueous solutions that require collections, separation, or removal of contaminants
 
If a larger pore size is required Pall supplies a AcroPrep 24-well filter plate with PP/PE media. This plate offers a pore size of 30-40 μm, making it ideal to use as a cell strainer when rapidly isolating primary cells. 

AcroPrep 24-well Filter Plates with Omega™ Membrane

AcroPrep 24-well filter plates with Omega membrane can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples. 

 

The Omega ultrafiltration membrane is a polyethersulfone (PES) membrane specifically modified to minimize protein and nucleic acid binding. The Omega membrane works on the premise of size exclusion, where samples are fractionated by molecular weight. Larger molecules can be separated from smaller molecules by selecting the appropriate molecular weight cut-off (MWCO) and filtering the sample through these plates by either centrifugation, vacuum, or positive pressure. Typical results are ≥ 90% recovery of target molecules.

 

Pall offers 24-well filter plates with a variety of MWCOs, ranging from 1 kDa to 100 Kda, to use with a wide range of biomolecules. Receiver plates are supplied with the filter plates to ensure the easy collection of the filtrate.

Applications

  • Clone selection and clone candidate analysis 
  • Cell expansion studies
  • Recombinant protein isolation prior to analysis
  • Cell clarification 
  • Process optimization 
  • Sterile filtration
  • Lysate clearance
  • Protein purification
  • Multiplexing assays
  • Aqueous filtration
  • Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA 
  • Clean-up of labelling and PCR reactions
  • Desalting and buffer exchange
  • Fractionation based on size exclusion

Specifications

Product Attributes Values
AcroPrep 24-well Filter Plates Materials of Construction

Filter Media:

Seitz Depth Media

Supor EKV (Hydrophilic polyethersulfone membrane)

 

Supor (Hydrophilic polyethersulfone membrane)

 

Omega (modified polyethersulfone membrane)

 

PP/PE non-woven (polypropylene/polyethylene) media

 

Plate Housing: Polypropylene

Lid: Polystyrene

Dimensions

Length: 12.8 cm (5.0 in)

Width: 8.6 cm (3.4 in)

Height (With receiver plate): 7.5 cm (2.9 in)

Height (Without receiver plate): 3.9 cm (1.5 in) 

Well-Bottom Area

1.56 cm2 (0.24 in2)
Recommended Working Volume (Max)

7 mL for vacuum

6 mL for centrifugation

Recommended Operating Vacuum

With Seitz Depth Media + Supor EKV:

≥ 25.4 cm Hg (10 in Hg) 

With Supor membrane:

≥ 38 cm Hg (15 in. Hg)

With Omega membrane:

≥ 38 cm Hg (15 in. Hg)

Recommended Centrifugal Force

With Seitz Depth Media + Supor EKV:

1,000 x g 

With Supor and Supor EKV membrane:

1,500 x g

With Omega membrane:

1,500 x g

Literature Library

  • Datasheet: AcroPrep 24-well Filter Plates for Cell Clarification and Sterile Filtration

    Select Language :
  • Data Sheet: AcroPrep 24-well Filter Plates With Supor Membrane

    Select Language :
  • Datasheet: AcroPrep 24-well Filter Plates With Omega Membrane

    Select Language :
  • Poster: Use of 24-Well Filter Plates for Medium Throughput High Volume Sample Preparation Applications

    Select Language :