24-well Filter Plates

Pall’s AcroPrep^TM 24-well filter plates feature a range of Pall’s proprietary membranes to meet the requirements of researcher’s applications and workflows. The filter plates feature a ridged and robust polypropylene construction that offers extensive drug and chemical compatibility. The low protein binding and low extractable properties of both filter plate and membrane materials ensure maximum recovery of valuable samples.

Utilizing Pall’s proprietary high-performance multi-layer filter media and membranes, these plates offer time savings, strong performance claims and streamlined workflow improvements in a 24-well plate format.

• Intrinsic plate and membrane properties minimize sample loss from non-specific binding 
• Automation friendly – compatible with all major automation platforms 
• Designed in accordance with the ANSI/SLAS X-2004 standards 
• Vacuum compatible – compatible with all popular vacuum and positive pressure manifolds 
• Centrifugation - suitable for centrifugation with compatible rotors

AcroPrep Cell Clarification and Sterile Filtration Plate Accelerating Protein Purification Workflows

• Offers workflow that is compatible with 24-well cell culture formats
• Depth filter plus 0.65/0.2 µm Supor EKV membrane 
• Demonstrates retention of 107 cfu/cm² of B. diminuta per modified ASTM F838 
• Depth media allows clarification of large cellular debris prior to filtration through the 0.65/0.2 µm Supor EKV membrane 
• Reliable recovery of > 90% of extra cellular proteins
• Uses up to seven times (7x) less plastic consumables by weight, significantly reducing disposal costs compared with traditional methods

Pall’s sterile filtration AcroPrep 24-well filter plate is well suited for the high volume (up to 7 mL) plate-based sterile filtration of media, reagents, serum or protein. The filter plate features a dual-layer, 0.65 μm membrane integrated with a highly asymmetric 0.2 μm membrane, this offers a fast, efficient, and sterilizing grade filtration.

Unlike alternative sterilizing grade filter plates available on the market, the Pall AcroPrep 0.2 μm Supor EKV Filtration Plates are individually bagged and gamma irradiated.

• Offers workflow that is compatible with 24-well cell culture formats
• 0.65/0.2 µm Supor EKV dual-layer membrane
• Process up to 7 mL of media, reagents, serum or protein in sterile filtrate applications
• Compatible for use with either a vacuum manifold or centrifuge
• Sterilizing grade plate, demonstrates retention of 107 cfu/cm² of B. diminuta per modified ASTM F838 
• Gamma irradiated and individual bagged (both filter and collection plate)

AcropPrep 24-well filter plates with Supor membrane can be used for a variety of applications. The Supor polyethersulfone (PES) membrane enables fast filtration, has low protein binding properties and offers extensive drug and chemical compatibility.

Available with 0.1, 0.45, 0.8, 1.2 and 5 μm pore size ratings, these versatile filter plates can be confidently incorporated into a number of laboratory 24- well format workflows. 

• Protein purification: High performance Supor membrane offers optimal support to retain chromatography sorbents while allowing smooth flow of buffers
• Lysate clearance and general sample preparation: Supor membrane is ideal for particulate removal or lysate clearance prior to downstream analysis or subsequent purifications 
• Multiplexing assays: These high-performance membranes do not trap microspheres in the membrane matrix, ensuring superior bead recovery and reducing false positives
• Aqueous filtration: The broad drug and chemical compatibility of the Supor membrane make it ideal for filtering aqueous solutions that require collections, separation, or removal of contaminants

AcroPrep 24-well filter plates with Omega membrane can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples. 

The Omega ultrafiltration membrane is a polyethersulfone (PES) membrane specifically modified to minimize protein and nucleic acid binding. The Omega membrane works on the premise of size exclusion, where samples are fractionated by molecular weight. Larger molecules can be separated from smaller molecules by selecting the appropriate molecular weight cut-off (MWCO) and filtering the sample through these plates by either centrifugation, vacuum, or positive pressure. Typical results are ≥ 90% recovery of target molecules.

Pall offers 24-well filter plates with a variety of MWCOs, ranging from 1 kDa to 100 Kda, to use with a wide range of biomolecules. Receiver plates are supplied with the filter plates to ensure the easy collection of the filtrate.

• Clone selection and clone candidate analysis 
• Cell expansion studies
• Recombinant protein isolation prior to analysis
• Cell clarification 
• Process optimization 
• Sterile filtration
• Lysate clearance
• Protein purification
• Multiplexing assays
• Aqueous filtration
• Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA 
• Clean-up of labelling and PCR reactions
• Desalting and buffer exchange
• Fractionation based on size exclusion