Filter Plates

The AcroPrep^TM line of multi-well filter plates provide a superior platform for a multitude of laboratory applications. 

 

AcroPrep filter plates are designed to support the stringent requirements for high throughput applications and comply with the standards set forth by the Society of Biomolecular Screening (ANSI/SBS x-2004) for multi-well plates. Pall filter plates feature a rigid construction, which facilitates ease of use in automated systems. The rigidity of the plate prevents it from flexing and jamming in robotic processing units.

 

Plates are constructed from chemically resistant and biologically inert polypropylene, which means that plates are themselves low in binding to nucleic acids and proteins. The nature of the polypropylene housing also provides durability when using harsh organic solvents and will not contribute unwanted extractables or leachables. Additionally, the materials of construction have been tested to ensure that they do not contribute to ion suppression/enhancement. 

 

The patented design and unique sealing mechanism used in AcroPrep filter plates individually seals each well to prevent crosstalk (cross contamination from one well to another). Specially engineered fluid directors and outlet tips on the bottom of AcroPrep filter plates are designed to further reduce the potential for crosstalk.

 

The proprietary sealing technology provides flexibility in integrally sealing a variety of membranes and media in filter plates. This flexibility has allowed Pall to develop a wide array of plates that can meet developing customer needs.

• Multi-well filter plates allow for high throughput and rapid sample processing
• Filter plates can be utilized across a number of life science and analytical applications and workflows
• Various filter choices – Specialized membranes and media accommodates a range of applications to guarantee success
• Optimized outlet tips – Minimizes sample leakage during incubation steps and reduces the presence of hanging drops following filtration
• Smooth well design – Provides consistency in filtration times, as well as efficient sample and bead recovery
• Optimized well geometry – Results in fast, uniform filtration rates across filter plates, thereby improving well-to-well, plate-to-plate, and lot-to-lot consistency
• Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run in manual, semi-automated, and automated processes

Pall supplies filter plates in 24, 96 and 384-well configurations with the following well volumes:

 

	Well Volume (max)	Recommended Working Volume
AcroPrepTM 24-well Filter Plates	7 mL	7 mL for vacuum 6 mL for centrifugation
AcroPrepTM Advance 96-well Filter Plates	350 μL	≤ 300 μL
AcroPrepTM Advance 96-well Filter Plates, 1 mL	1 mL	≤ 900 μL
AcroPrepTM Advance 96-well Filter Plates, 2 mL	2 mL	≤ 1.9 mL
AcroPrepTM 384-well Filter Plates	100 µL	80 µL

 

Pall also offers a multi-well plate vacuum manifold that has been designed and optimized for vacuum filtration, and is a perfect fit for SBS-conforming filter plates. 

 

The manifold is constructed from durable anodized aluminum and comes complete with the necessary O-ring and gasket. The control block includes the vacuum pressure gauge, vacuum metering valve, vacuum release valve and the 1/4 inch hose barb for vacuum line attachment. Included with the vacuum manifold unit is a spacer block designed to accommodate standard 350 µL receiver plates. A spacer block that has been optimized to work with our 1 mL plate is available separately. 

Field	Applications	Membrane
Diagnostics	Multiplex Assays	Supor® (polyethersulfone) membrane
Diagnostics	Flow Cytometry Sample Prep	PP/PE non-woven (polypropylene/polyethylene) media
Nucleic Acids	Plasmid DNA purification	Silica-based quartz glass fiber
Nucleic Acids	Genomic Purification	Silica-based quartz glass fiber
Nucleic Acids	Total RNA Purification	Silica-based quartz glass fiber
Nucleic Acids	PCR Clean-up	OmegaTM (modified polyethersulfone) membrane
Nucleic Acids	Labeling Clean-up	Omega (modified polyethersulfone) membrane
Proteins	Lysate Clarification	Glass Fiber/Supor (polyethersulfone) membrane
Proteins	Recombinant protein isolation	Seitz® Depth Media/Supor EKV (hydrophilic polyethersulfone membrane)
Proteins	Cell Clarification	Seitz Depth Media/Supor EKV (hydrophilic polyethersulfone membrane)
Proteins	Chromatography Screening	Supor (polyethersulfone) membrane
Proteins	Size Exclusion	Omega (modified polyethersulfone) membrane
Proteins	Concentration, Buffer Exchange, Desalting	Omega (modified polyethersulfone) membrane
Proteins	Protein Precipitation	PTFE, wwPTFE (water-wettable polytetrafluoroethylene)
Proteins	Sample Fractionation	Mustang® Q, Mustang S
Proteins	Free vs. Bound Assays	Omega (modified polyethersulfone) membrane
Proteins	Solid Phase ELISA	BioTrace™ NT (nitrocellulose)
General Filtration	Aqueous Filtration	Supor (polyethersulfone) membrane, wwPTFE (water-wettable polytetrafluoroethylene)
General Filtration	Sterile Filtration	Supor EKV (Hydrophilic polyethersulfone membrane)
General Filtration	Particulate Removal	Glass Fiber
General Filtration	Cell Separation	PP/PE non-woven (polypropylene/polyethylene) media
General Filtration	Solvent Filtration	PTFE, wwPTFE (water-wettable polytetrafluoroethylene)