Pall’s Acro 50 vent devices are available in two membrane variants – one with PTFE membrane and the other with Emflon II PVDF membrane - are convenient prefabricated devices used for sterilization of air, gases or as a bacterial air vent in various research, pharmaceutical and laboratory processes.
Special Features –
- Lightweight and self-supporting
- High heat stability
- Heat-sealed to ensure ‘no leaching’
- High flow rate
- Available in a variety of inlet/ outlet connections and pore sizes to best match your application requirements
- Industry-standard for venting bioreactors, flasks and carboys
- Broad chemical compatibility
Advantages of Pall’s Acro 50 vent filters –
- Self-contained and compact
- Remove particulate matter and airborne bacteria through its 50 mm vent
- Work in both dry and moist conditions
- The hydrophobic membrane prevents the passage of aerosolized pathogens and water into sensitive equipment and protects the laboratory environment
- Protect equipment and staff
- Rugged polypropylene housing has broad chemical compatibility
- Disposable – Eliminates the cost and labor associated with disc-loaded stainless-steel filter holders
Material of Construction
PTFE on a polypropylene support for Acro 50 vent devices with PTFE membrane
Hydrophobic polyvinylidene for (PVDF) Acro 50 vent devices with Emflon® II Membrane
Effective Filtration Area
PTFE membrane - 19.6 cm2
Emflon II membrane - 20 cm2
Typical Air Flow Rates
0.2 µm: 8 Lpm at 0.2 bar (20 kPa, 3 psi)
0.45 µm: 12 Lpm at 0.2 bar (20 kPa, 3 psi)
1 µm: 15 Lpm at 0.2 bar (20 kPa, 3 psi)
Overall Length: 8.2 cm (3.2 in.)
Diameter: PTFE membrane - 7.3 cm (2.9 in.); Emflon II membrane - 6.8 cm (2.7 in.)
Inlet/ Outlet Connections:
Stepped hose barbs, 6.4 -12.7 mm (1/4-1/2 in.) diameter. Connections have internal taper design to accept male slip, Luer. The 0.2 µm pore size PTFE devices are available with 1/8 in. MNPT or 3/8 in. (9.5 mm) OD straight type connections. The 1 µm pore size device is also offered with a 1/8 in. MNPT connection.
Maximum Operating Temperature
PTFE membrane - 130 OC (266 OF) at 1.0 bar (100 kPa, 15 psi)
Emflon II membrane - 60 °C (140 °F) at 1.0 bar (100 kPa, 15 psi)
Maximum Operating Pressure
4.1 bar (410 kPa, 60 psi) at ambient temperature
Minimum Bubble Point (IPA, 100%)
0.2 µm: 1.03 bar (103 kPa, 15 psi)
0.45 µm: 0.34 bar (34 kPa, 5 psi)
1 µm: 0.21 bar (21 kPa, 3 psi)
Emflon II membrane
[60% IPA/40% Water (v:v)]: 1.1 bar (110 kPa, 16 psi)
Autoclaving - at 121-123 OC (250-253 OF) at approximately 1.0 bar (100 kPa, 15 psi) for a maximum of 20 minutes. Integrity should be verified after each autoclave cycle and before use
Note: It may be necessary to run a drying cycle or air purge to remove residual condensation from the product before using it. Reuse requires individual integrity testing and consideration for other problems including cross-contamination.
- Venting bioreactors, fermentation tanks, and carboys
- Sterile gas purging of culture vessels
- Filtering aggressive solvents
- Sterile air for cell factories
- Venting of sterile collection vessels
What is the recommended method of integrity testing for Pall’s 50 mm vent devices?
Bubble Point Method (BPM) – BPM is a procedure which measures the pressure needed to be applied to the upstream side of a filter causing bulk or open pore flow through the largest pores of a wet filter.
Water Breakthrough Test (WBT) – This simple and reproducible integrity test is adopted by membrane manufacturers and reported in their literature for hydrophobic membranes. The hydrophobic PTFE membrane resists water penetration; however, water can be forced through it under high pressure. WBT kits are available from Pall Laboratory. WBT test cannot be performed on units after an alcohol Bubble Point Test due to residual alcohol “wetting out” the membrane.
Where are Acro 50 mm vent devices used?
Pall’s Acro 50 mm vent devices are used in applications where sterility is a major concern, as they are integrity testable.