Macrosep Advance Centrifugal Devices with Omega Membrane 100K, clear (24/pkg)
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Macrosep Advance Centrifugal Devices with Omega Membrane 100K, clear (24/pkg)

Product ID: MAP100C37Need this product with different specifications

Min Order Qty: 1
$ 238.00
Product Group
Macrosep® Advance Centrifugal Devices
Sample Volume
5 to 20 mL
Sample Resevoir Material
Polypropylene
Filter Media
Omega (modified polyethersulfone)
MWCO or pore size
100 K
Effective Filtration Area
7.2 cm²
Hold Up Volume
80 μL

Description

Macrosep Advance Centrifugal Devices with Omega Membrane 1uickly concentrates up to 20 mL of biological sample. Exchange buffer or remove salt of chromatography eluates and gradient fractions., Pall Macrosep Advance Centrifugal Devices with Omega Membrane, 100K, clear (24/pkg)
“Pall Laboratory products are designed for laboratory applications only. This product is not approved for use in medical, clinical, surgical or other patient applications. If you require further assistance in product selection for your chosen application, please contact Labcustomersupport@pall.com or call 1-800-521-1520."

Applications

Ultrafiltration

  • Concentrate and desalt proteins
  • Exchange buffer or remove salt of chromatography eluates and gradient fractions
  • Recover proteins or other molecules from cell culture supernatants

Microfiltration

  • Remove particulate from aqueous solutions and clinical samples

Specifications

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
  • Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
  • Paddle: Polyethylene

Effective Filtration Area

  • 7.2 cm2

Dimensions

  • Diameter: 29 mm (1.2 in.)
  • Length: 12.0 cm (4.7 in.)

Operating Temperature Range

  • 0 - 40 °C (32 - 104 °F)

Capacities

  • Maximum Sample Volume: 20 mL
  • Final Concentrate Volume:
       450 μL (swinging bucket)
       1.2 - 1.5 mL (45º angle rotor)
       1.5 mL (34º angle rotor)
  • Filtrate Receiver Volume: 22 mL
  • Hold-Up Volume: 80 μL (membrane and paddle)

pH Range

  • 1 - 14

Maximum Centrifugal Force

  • 5,000 x g (ultrafiltration)
  • 14,000 x g (microfiltration)

Centrifuge

  • Fits centrifuges that accept standard 50 mL conical end tubes

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.

Performance

Macrosep Advance Centrifugal Devices: Reduced Spin Time



Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.

Macrosep Advance Centrifugal Devices: Retention Efficiency


Macrosep Advance Centrifugal Devices: Retention Efficiency
Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.

Type

Centrifugal Filters

Use

Sample Concentration, Desalting, Buffer exchange

Application

Plasmid Purification